##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727157.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5613804
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.485232651514018	31.0	31.0	34.0	30.0	34.0
2	31.696813782597324	31.0	31.0	34.0	30.0	34.0
3	31.8676674497364	31.0	31.0	34.0	30.0	34.0
4	35.578137747595036	37.0	35.0	37.0	33.0	37.0
5	35.380397142472376	37.0	35.0	37.0	33.0	37.0
6	35.40094898218748	37.0	35.0	37.0	33.0	37.0
7	35.292163744940154	37.0	35.0	37.0	32.0	37.0
8	35.26848390146859	37.0	35.0	37.0	32.0	37.0
9	36.775918788757146	39.0	37.0	39.0	32.0	39.0
10	36.574121219764706	39.0	35.0	39.0	32.0	39.0
11	36.64639253525773	39.0	35.0	39.0	32.0	39.0
12	36.48074888257588	38.0	35.0	39.0	32.0	39.0
13	36.48173555756489	38.0	35.0	39.0	32.0	39.0
14	37.429101372260234	39.0	36.0	41.0	32.0	41.0
15	37.36546983115193	39.0	36.0	41.0	32.0	41.0
16	37.273023603959096	39.0	36.0	41.0	31.0	41.0
17	37.6143867865711	39.0	36.0	41.0	32.0	41.0
18	37.656335347653744	39.0	37.0	41.0	32.0	41.0
19	37.74917346597779	40.0	37.0	41.0	32.0	41.0
20	37.72928018149547	40.0	37.0	41.0	32.0	41.0
21	37.689604767106225	40.0	37.0	41.0	32.0	41.0
22	37.632988611643725	40.0	37.0	41.0	32.0	41.0
23	37.50918379052778	39.0	37.0	41.0	32.0	41.0
24	37.396747731128485	39.0	36.0	41.0	31.0	41.0
25	37.31302981008956	39.0	36.0	41.0	31.0	41.0
26	37.02875251790052	39.0	36.0	41.0	31.0	41.0
27	36.84622298890378	39.0	36.0	41.0	30.0	41.0
28	36.63061392952088	39.0	36.0	40.0	30.0	41.0
29	36.45230667119835	39.0	35.0	40.0	30.0	41.0
30	36.31131582078747	39.0	35.0	40.0	29.0	41.0
31	36.14434882300843	39.0	35.0	40.0	29.0	41.0
32	35.97594483170413	38.0	35.0	40.0	27.0	41.0
33	35.809920510228004	38.0	35.0	40.0	27.0	41.0
34	35.69154053116211	38.0	35.0	40.0	27.0	41.0
35	35.52838912794248	38.0	34.0	40.0	26.0	41.0
36	35.41380283315912	38.0	34.0	40.0	25.0	41.0
37	35.33740900109801	38.0	34.0	40.0	25.0	41.0
38	35.22156420138644	38.0	34.0	40.0	25.0	41.0
39	35.07320561957631	38.0	34.0	40.0	24.0	41.0
40	34.97298302541378	38.0	34.0	40.0	24.0	41.0
41	34.74412323622271	38.0	33.0	40.0	23.0	41.0
42	34.56142679723054	38.0	33.0	40.0	23.0	41.0
43	34.27685683361941	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	7.0
11	38.0
12	71.0
13	60.0
14	119.0
15	211.0
16	353.0
17	550.0
18	1089.0
19	2120.0
20	3870.0
21	7081.0
22	12347.0
23	20063.0
24	29533.0
25	41805.0
26	56822.0
27	75391.0
28	97780.0
29	122998.0
30	153279.0
31	188528.0
32	231408.0
33	282120.0
34	347422.0
35	427974.0
36	544248.0
37	716288.0
38	1087180.0
39	1163041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.07377172412859	21.095535219968493	13.147448681856366	25.68324437404655
2	18.83042229475771	23.299798140440956	34.21309329645282	23.65668626834852
3	18.905736644884648	25.7064372037214	30.44158648930387	24.946239662090093
4	14.453425876642648	16.716401213864966	34.72517031232298	34.1050025971694
5	12.814145274754873	39.75137001576827	34.105145103035305	13.32933960644155
6	33.54196192100758	38.01409881784259	14.979860358502005	13.464078902647831
7	28.243112869633496	32.47776374094999	21.13570406091841	18.14341932849811
8	27.154029602743524	36.23338114405134	19.178635378078752	17.433953875126385
9	28.139457665426153	14.10102668351086	18.20337867157457	39.556136979488414
10	16.21392553070966	28.11033302908331	33.12539946175534	22.550341978451687
11	36.93310275884231	22.70155851540239	20.478662952963802	19.8866757727915
12	21.203216214887448	26.352808184966914	29.656486047606933	22.787489552538705
13	30.042819450055614	20.42627423401316	24.81426141703558	24.71664489889565
14	22.375487280995205	21.138055407705718	24.997844598778297	31.48861271252078
15	25.108625096280523	28.73575564804186	21.79596936408895	24.359649891588663
16	24.344615522736454	26.940591442095236	24.405608033340673	24.309185001827636
17	23.688999473440823	27.30526395292746	24.79320617534919	24.21253039828252
18	23.997595926042305	26.07294447757706	26.06264842876595	23.86681116761469
19	24.907727451831235	26.15278338894625	25.767536593725037	23.171952565497477
20	24.169030482717243	25.306654810178625	25.84110524699473	24.683209460109403
21	24.586412350698385	26.035697006878046	26.08251018382544	23.295380458598128
22	24.846877447092915	25.25016904758342	25.293686776381936	24.60926672894173
23	23.68379800933556	25.538654359860086	26.06995185439321	24.707595776411146
24	23.85400345291713	25.874807884279537	25.86878701144536	24.402401651357973
25	24.448003528445238	25.1746231254244	25.412126964176164	24.9652463819542
26	24.12081006034411	25.851971319269428	25.909383369992966	24.117835250393494
27	25.002137587988464	25.822294472696232	25.02126900048523	24.154298938830067
28	23.9498208345001	26.314955064337838	25.466938282847067	24.268285818314997
29	24.138623293581322	26.19826057340085	26.001442159362888	23.66167397365494
30	24.66717755019591	26.00938686138668	25.570878498786204	23.752557089631203
31	24.763101811178302	25.520662994290504	25.647885106070678	24.068350088460516
32	23.955645761768668	26.033595045356055	25.329402309022548	24.681356883852732
33	23.86551080158837	25.369606776438935	25.739445124909956	25.02543729706274
34	24.466760863044026	25.728151535037565	26.19578453398088	23.609303067937535
35	24.623624194930922	25.666214923071774	25.940609967857803	23.769550914139504
36	24.05791153378351	25.84992279744715	26.0215532996877	24.070612369081644
37	24.188963490709686	25.75750774341249	26.30980703993228	23.743721725945544
38	24.445545302258502	24.99130714218024	26.206632793022344	24.356514762538914
39	24.121647282306256	25.263546785744566	26.27068917974336	24.344116752205814
40	24.57844983544135	25.116908249735832	26.501156791366427	23.803485123456394
41	23.35199091382599	25.69918721779385	26.721809311475788	24.227012556904373
42	24.170170529644427	25.647742600204783	26.63350911431892	23.54857775583187
43	23.526346840751835	24.906658657837003	26.465868776323504	25.10112572508766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2117.0
1	1975.0
2	1833.0
3	1853.0
4	1873.0
5	1873.0
6	2458.5
7	3044.0
8	3683.5
9	4323.0
10	5784.5
11	7246.0
12	7246.0
13	14111.5
14	20977.0
15	31092.5
16	41208.0
17	36787.0
18	32366.0
19	32366.0
20	38921.0
21	45476.0
22	42705.5
23	39935.0
24	48478.0
25	57021.0
26	57021.0
27	69054.5
28	81088.0
29	92595.5
30	104103.0
31	118840.5
32	133578.0
33	133578.0
34	154877.5
35	176177.0
36	204490.0
37	232803.0
38	261756.5
39	290710.0
40	290710.0
41	312017.0
42	333324.0
43	350810.5
44	368297.0
45	402393.0
46	436489.0
47	436489.0
48	478096.0
49	519703.0
50	519874.0
51	520045.0
52	489997.0
53	459949.0
54	459949.0
55	436359.0
56	412769.0
57	388754.0
58	364739.0
59	333198.0
60	301657.0
61	301657.0
62	256190.0
63	210723.0
64	180171.0
65	149619.0
66	124801.5
67	99984.0
68	99984.0
69	80270.0
70	60556.0
71	51300.0
72	42044.0
73	32953.5
74	23863.0
75	23863.0
76	18330.0
77	12797.0
78	10635.0
79	8473.0
80	6431.0
81	4389.0
82	4389.0
83	3633.0
84	2877.0
85	2381.0
86	1885.0
87	1794.5
88	1704.0
89	1704.0
90	1227.0
91	750.0
92	611.5
93	473.0
94	518.5
95	564.0
96	564.0
97	328.0
98	92.0
99	126.5
100	161.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5613804.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.70485013372057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.89291335318586	41.82555961806518
2	9.817826358159792	10.152584809750872
3	3.4499525008515817	5.351378310749564
4	1.62677447711866	3.3644852216312784
5	0.9451976448601123	2.443565128711887
6	0.6202993561261728	1.9243491147928278
7	0.4461777512261417	1.6148687632103678
8	0.34332437008002203	1.4201228081793245
9	0.2441297941380464	1.1360424977175367
>10	1.4411525876912894	13.813937659884912
>50	0.10221621188748382	3.6154563616572615
>100	0.059041206941967836	5.867801551072694
>500	0.006638128915297705	2.4369026026564717
>1k	0.004114244438713628	3.719905303732755
>5k	1.3829393070982878E-4	0.4434540848006305
>10k+	1.0372044803237161E-4	0.8695861633864097
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21050	0.3749685596433363	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15456	0.27532133291436606	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12139	0.21623483826653014	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7857	0.13995857354478353	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6286	0.11197398412912171	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	5642	0.10050226192435646	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.7813233237213126E-5	0.0
5	0.0	0.0	0.0	1.7813233237213126E-5	0.0
6	3.562646647442625E-5	0.0	0.0	1.7813233237213126E-5	1.7813233237213126E-5
7	3.562646647442625E-5	0.0	0.0	3.562646647442625E-5	3.562646647442625E-5
8	3.562646647442625E-5	0.0	0.0	3.562646647442625E-5	5.343969971163938E-5
9	3.562646647442625E-5	0.0	0.0	5.343969971163938E-5	7.12529329488525E-5
10	3.562646647442625E-5	0.0	0.0	1.42505865897705E-4	7.12529329488525E-5
11	3.562646647442625E-5	0.0	0.0	1.42505865897705E-4	8.906616618606563E-5
12	3.562646647442625E-5	0.0	0.0	1.42505865897705E-4	1.0687939942327876E-4
13	3.562646647442625E-5	0.0	0.0	1.6031909913491815E-4	1.0687939942327876E-4
14	3.562646647442625E-5	0.0	0.0	1.6031909913491815E-4	2.1375879884655752E-4
15	3.562646647442625E-5	0.0	0.0	1.7813233237213126E-4	2.493852653209838E-4
16	5.343969971163938E-5	0.0	0.0	2.3157203208377066E-4	3.562646647442625E-4
17	5.343969971163938E-5	0.0	0.0	2.8501173179541E-4	3.7407789798147564E-4
18	5.343969971163938E-5	0.0	0.0	3.206381982698363E-4	3.918911312186888E-4
19	5.343969971163938E-5	0.0	0.0	4.4533083093032815E-4	3.918911312186888E-4
20	5.343969971163938E-5	0.0	0.0	5.343969971163938E-4	3.918911312186888E-4
21	5.343969971163938E-5	0.0	0.0	7.12529329488525E-4	4.097043644559019E-4
22	5.343969971163938E-5	0.0	0.0	9.79727828046722E-4	4.097043644559019E-4
23	5.343969971163938E-5	0.0	0.0	0.0014428718922142633	6.056499300652463E-4
24	5.343969971163938E-5	0.0	0.0	0.002262280621126067	6.234631633024594E-4
25	5.343969971163938E-5	0.0	0.0	0.0028857437844285266	6.412763965396726E-4
26	5.343969971163938E-5	0.0	0.0	0.0040614171780845925	6.590896297768857E-4
27	5.343969971163938E-5	0.0	0.0	0.01173892070332345	6.590896297768857E-4
28	5.343969971163938E-5	0.0	0.0	0.026452651357261492	6.590896297768857E-4
29	5.343969971163938E-5	0.0	0.0	0.047828531241917244	6.769028630140988E-4
30	5.343969971163938E-5	0.0	0.0	0.07898387617380301	6.769028630140988E-4
31	5.343969971163938E-5	0.0	0.0	0.19197321459744587	6.769028630140988E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1560	0.0	16.246796	5
CAAGACG	1730	0.0	16.040462	4
CGAACGA	770	0.0	15.376625	16
TATACTG	855	0.0	15.362573	5
ACGAACG	785	0.0	15.082803	15
CGCAAGA	1855	0.0	14.959569	2
ATACCGT	1345	0.0	14.855019	6
GTATTAG	1295	0.0	14.428571	1
TCGTTAC	990	0.0	14.388889	23
TACCGTC	1280	0.0	14.1640625	7
CGAACGT	420	0.0	14.095238	4
ACGGATG	2075	0.0	14.086747	28
TTAGAGT	975	0.0	14.041025	4
ACGGACC	1780	0.0	13.926966	8
TACGGAT	2060	0.0	13.919902	27
TATGCGA	120	3.304851E-5	13.874999	12
GTTACGG	1565	0.0	13.7124605	25
CGTCGTA	1255	0.0	13.709164	10
GCGCAAG	1960	0.0	13.591836	1
ACCGTCG	1280	0.0	13.5859375	8
>>END_MODULE