##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727156.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3101296
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.008331033219662	31.0	31.0	33.0	30.0	34.0
2	31.256417317147413	31.0	31.0	34.0	30.0	34.0
3	31.390666031233394	31.0	31.0	34.0	30.0	34.0
4	35.14887679215399	35.0	35.0	37.0	32.0	37.0
5	34.90143733458528	35.0	35.0	37.0	32.0	37.0
6	35.036841372123135	36.0	35.0	37.0	32.0	37.0
7	34.9514938270968	36.0	35.0	37.0	32.0	37.0
8	34.92874204848554	36.0	35.0	37.0	32.0	37.0
9	36.396356233007104	38.0	35.0	39.0	32.0	39.0
10	36.076321641017174	38.0	35.0	39.0	31.0	39.0
11	36.228004679334056	38.0	35.0	39.0	32.0	39.0
12	36.015375829975596	37.0	35.0	39.0	30.0	39.0
13	36.05332351378262	37.0	35.0	39.0	31.0	39.0
14	36.913496809075944	38.0	36.0	40.0	31.0	41.0
15	36.84867165210931	38.0	36.0	40.0	31.0	41.0
16	36.748853705031706	38.0	36.0	40.0	31.0	41.0
17	37.03736405683301	39.0	36.0	40.0	31.0	41.0
18	37.060236430189185	39.0	36.0	40.0	31.0	41.0
19	37.1217952107764	39.0	36.0	40.0	31.0	41.0
20	37.081437244300446	39.0	36.0	40.0	31.0	41.0
21	37.016050709122894	39.0	36.0	40.0	31.0	41.0
22	36.94273039400302	39.0	36.0	40.0	31.0	41.0
23	36.780909335967934	39.0	36.0	40.0	30.0	41.0
24	36.643505166872174	39.0	36.0	40.0	30.0	41.0
25	36.56457719611414	39.0	35.0	40.0	30.0	41.0
26	36.226971240410464	38.0	35.0	40.0	29.0	41.0
27	36.001540001341375	38.0	35.0	40.0	28.0	41.0
28	35.76199659755147	38.0	34.0	40.0	27.0	41.0
29	35.549364523734596	38.0	34.0	40.0	27.0	41.0
30	35.382482033317686	38.0	34.0	40.0	26.0	41.0
31	35.2068760930914	38.0	34.0	40.0	25.0	41.0
32	35.02649376260763	38.0	34.0	40.0	25.0	41.0
33	34.83199217359452	38.0	33.0	40.0	25.0	41.0
34	34.69068834448566	38.0	33.0	40.0	24.0	41.0
35	34.50619450707059	38.0	33.0	40.0	24.0	41.0
36	34.37845855410125	38.0	33.0	40.0	24.0	41.0
37	34.28163483911242	38.0	33.0	40.0	23.0	41.0
38	34.145327630771135	38.0	33.0	40.0	23.0	41.0
39	33.984126958536045	38.0	32.0	40.0	22.0	41.0
40	33.85573321604903	37.0	32.0	40.0	21.0	41.0
41	33.59959384721742	37.0	32.0	40.0	19.0	41.0
42	33.38572583848817	37.0	31.0	40.0	18.0	41.0
43	33.09359506477292	37.0	31.0	40.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	13.0
11	40.0
12	48.0
13	59.0
14	101.0
15	202.0
16	358.0
17	544.0
18	1050.0
19	2050.0
20	3772.0
21	6407.0
22	10478.0
23	16375.0
24	23301.0
25	32049.0
26	42638.0
27	54810.0
28	68537.0
29	84559.0
30	102912.0
31	125070.0
32	150117.0
33	180241.0
34	218753.0
35	267275.0
36	339770.0
37	451240.0
38	634997.0
39	283526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.09317008115317	24.4033784585541	14.823867183267897	23.67958427702483
2	21.44403500987974	25.329184960094103	31.004747692577556	22.222032337448603
3	21.326310032966862	26.354079068879592	29.499828458812054	22.819782439341488
4	16.79414025620257	18.525932384396718	33.21488822737333	31.465039132027385
5	13.207575155676851	40.05306168775892	33.80090130061755	12.938461855946676
6	33.661443473954115	39.166303377684685	14.122450743173175	13.049802405188023
7	28.197405213820286	32.39274806403517	21.227286914889774	18.182559807254773
8	27.010159623589626	36.40242659842853	18.861114837151952	17.7262989408299
9	27.55967182751985	13.828638091946077	18.099562247524904	40.512127833009174
10	16.069088535889513	28.311551041887007	33.421382544587814	22.197977877635672
11	37.65716010338904	22.448840742708857	20.207648673328826	19.686350480573285
12	20.917738906573252	26.798570662071597	29.527365333718546	22.7563250976366
13	30.23136134054924	20.400116596416463	24.739850694677322	24.628671368356972
14	22.43307314103523	21.212905830336737	24.669170566111717	31.684850462516316
15	25.066520577203853	29.110700816690827	21.363552527717445	24.459226078387875
16	24.78541229215141	27.001453585855717	23.87627624064262	24.336857881350248
17	23.81423766064252	27.681266154536683	24.561538144053323	23.942958040767472
18	24.42691700501984	25.74688130381621	25.76026280625906	24.06593888490489
19	24.90739355417864	26.295716371478246	25.430561932817763	23.36632814152535
20	24.588268904354823	25.28326867219382	25.444749549865602	24.683712873585755
21	25.066488332619652	26.16402948960693	25.173250150904654	23.596232026868766
22	25.027988299085287	25.742979709128054	25.049624415083244	24.179407576703415
23	23.96294968297125	25.466353421279365	25.641667225572796	24.929029670176597
24	24.20858892540409	26.16986575934706	25.34440440383633	24.27714091141252
25	24.61561231175612	25.482346735042384	25.102892468181043	24.799148485020456
26	24.54064365349196	26.007385299565083	25.303292558981795	24.148678487961163
27	25.19933601952216	25.781479742662423	24.8132393683157	24.205944869499717
28	24.536580835882805	25.99445522130103	25.44842543246436	24.020538510351802
29	24.59778105669372	25.881728154939097	25.614259329003104	23.906231459364086
30	24.54509340611151	26.197983036769145	25.355690008306205	23.901233548813142
31	25.135685210312076	25.60968059804675	25.167768571590717	24.086865620050457
32	24.256955801703544	25.955761720261467	25.023312834376338	24.76396964365865
33	24.20230123148516	25.57414706625875	25.440847955177446	24.782703747078642
34	24.885467236922885	25.64608473360814	25.69603159453338	23.77241643493559
35	24.951955569542537	25.735918145188336	25.596847253535298	23.71527903173383
36	24.394962622077994	25.921034303078454	25.48669975390933	24.197303320934218
37	24.670847284490097	25.678845231154973	25.62767307603015	24.022634408324777
38	24.421822360716295	25.02328058979214	26.09412323106211	24.460773818429455
39	24.80862839277515	25.11237237593574	25.56424797890946	24.51475125237965
40	24.630122374645953	25.334376338150243	25.98671652109312	24.048784766110685
41	23.824168992576006	25.593558305946935	26.303487316270356	24.2787853852067
42	24.810208377400933	25.515203966341815	26.057170937569325	23.617416718687927
43	24.2520868694894	24.927933354313808	26.124787830635967	24.695191945560822
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	651.0
1	569.0
2	487.0
3	480.0
4	473.0
5	473.0
6	721.5
7	970.0
8	1258.5
9	1547.0
10	2230.0
11	2913.0
12	2913.0
13	6363.5
14	9814.0
15	14746.5
16	19679.0
17	16958.0
18	14237.0
19	14237.0
20	18392.0
21	22547.0
22	22191.5
23	21836.0
24	26390.0
25	30944.0
26	30944.0
27	37735.5
28	44527.0
29	51102.5
30	57678.0
31	66655.5
32	75633.0
33	75633.0
34	87658.5
35	99684.0
36	116326.5
37	132969.0
38	148815.0
39	164661.0
40	164661.0
41	177064.5
42	189468.0
43	199634.0
44	209800.0
45	226951.5
46	244103.0
47	244103.0
48	264745.0
49	285387.0
50	282624.0
51	279861.0
52	264059.5
53	248258.0
54	248258.0
55	235306.5
56	222355.0
57	210873.5
58	199392.0
59	182771.5
60	166151.0
61	166151.0
62	141335.0
63	116519.0
64	100351.0
65	84183.0
66	71154.0
67	58125.0
68	58125.0
69	46395.5
70	34666.0
71	30086.5
72	25507.0
73	20101.5
74	14696.0
75	14696.0
76	11263.0
77	7830.0
78	6570.0
79	5310.0
80	4002.5
81	2695.0
82	2695.0
83	2272.5
84	1850.0
85	1599.5
86	1349.0
87	1288.5
88	1228.0
89	1228.0
90	865.0
91	502.0
92	425.0
93	348.0
94	345.5
95	343.0
96	343.0
97	200.0
98	57.0
99	60.0
100	63.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3101296.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.25636822807951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.37211695088715	46.90212511143616
2	8.71685225099665	9.807569000436626
3	2.9225776429460155	4.9324081217017115
4	1.3892773591199594	3.126227947423452
5	0.8223762631269795	2.3131950940251684
6	0.5218009811395987	1.7612776882057462
7	0.3775946415192854	1.4869472235981018
8	0.2831606880275996	1.2743673546717589
9	0.2077721318546285	1.0519655001432362
>10	1.250887299310192	12.742656419266552
>50	0.07632862714859096	2.9512699470191053
>100	0.04929832108297731	5.469245958238452
>500	0.006809564931457127	2.6349883360690876
>1k	0.003032831439575402	3.005257425918967
>5k	1.144464694179397E-4	0.5404988718459585
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9711	0.3131271571626829	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7079	0.2282594115492362	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4825	0.15558011876325253	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3914	0.12620530255738246	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3744	0.12072372324344403	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGG	3201	0.1032149140230407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.224458419963783E-5
2	0.0	0.0	0.0	0.0	3.224458419963783E-5
3	0.0	0.0	0.0	0.0	3.224458419963783E-5
4	0.0	0.0	0.0	0.0	3.224458419963783E-5
5	0.0	0.0	0.0	0.0	3.224458419963783E-5
6	0.0	0.0	0.0	0.0	3.224458419963783E-5
7	0.0	0.0	0.0	0.0	3.224458419963783E-5
8	0.0	0.0	0.0	0.0	3.224458419963783E-5
9	0.0	0.0	0.0	0.0	1.6122292099818915E-4
10	0.0	0.0	0.0	0.0	1.6122292099818915E-4
11	0.0	0.0	0.0	3.224458419963783E-5	1.6122292099818915E-4
12	0.0	0.0	0.0	3.224458419963783E-5	1.6122292099818915E-4
13	0.0	0.0	0.0	3.224458419963783E-5	1.9346750519782697E-4
14	0.0	0.0	0.0	3.224458419963783E-5	2.579566735971026E-4
15	0.0	0.0	0.0	3.224458419963783E-5	2.579566735971026E-4
16	3.224458419963783E-5	0.0	0.0	6.448916839927566E-5	3.8693501039565394E-4
17	3.224458419963783E-5	0.0	0.0	9.673375259891348E-5	4.191795945952918E-4
18	3.224458419963783E-5	0.0	0.0	9.673375259891348E-5	4.836687629945674E-4
19	3.224458419963783E-5	0.0	0.0	9.673375259891348E-5	4.836687629945674E-4
20	3.224458419963783E-5	0.0	0.0	1.6122292099818915E-4	4.836687629945674E-4
21	3.224458419963783E-5	0.0	0.0	3.546904261960161E-4	4.836687629945674E-4
22	3.224458419963783E-5	0.0	0.0	6.448916839927566E-4	4.836687629945674E-4
23	3.224458419963783E-5	0.0	0.0	0.0010640712785880484	7.093808523920322E-4
24	3.224458419963783E-5	0.0	0.0	0.00148325087318334	7.4162543659167E-4
25	3.224458419963783E-5	0.0	0.0	0.0019991642203775455	7.4162543659167E-4
26	3.224458419963783E-5	0.0	0.0	0.0029020125779674047	7.4162543659167E-4
27	3.224458419963783E-5	0.0	0.0	0.007222786860718874	7.4162543659167E-4
28	3.224458419963783E-5	0.0	0.0	0.015638623336824347	7.4162543659167E-4
29	3.224458419963783E-5	0.0	0.0	0.027891565332686722	7.4162543659167E-4
30	3.224458419963783E-5	0.0	0.0	0.04852809922045493	7.4162543659167E-4
31	3.224458419963783E-5	0.0	0.0	0.12175554993783244	7.738700207913079E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAG	50	0.007037512	18.499998	28
AAGACGG	1235	0.0	17.376518	5
CAAGACG	1260	0.0	17.178572	4
CCGATAA	475	0.0	15.968421	9
CGCAAGA	1335	0.0	15.93633	2
TATCTAG	875	0.0	15.857143	1
TAACGAA	470	0.0	15.744679	13
GCGCAAG	1360	0.0	15.507353	1
ACGGACC	1390	0.0	15.172662	8
GACGGAC	1420	0.0	15.112676	7
CGATAAC	490	0.0	15.102041	10
ACGAACG	485	0.0	14.876288	15
GTTATCT	610	0.0	14.860655	3
CGGACCA	1420	0.0	14.852113	9
GGTTATC	600	0.0	14.8	2
GCAAGAC	1650	0.0	14.68788	3
TAGAGTC	970	0.0	14.685567	5
ACCGTCG	895	0.0	14.4692745	8
AGACGGA	1465	0.0	14.395905	6
AACGAAC	515	0.0	14.368932	14
>>END_MODULE