FastQCFastQC Report
Thu 9 Feb 2017
SRR2727155.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727155.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4034226
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT264950.6567554718054962No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG177760.44062975153102474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169320.4197087619781341No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC166890.4136853017158682No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA119620.29651288747829196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112280.278318567179925No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG100020.24792859894314298No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT91670.22723070051107697No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG88040.21823269197114883No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG85870.21285371716904308No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC78090.1935687291688666No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG67470.16724397691155626No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64700.1603777279706194No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC63540.15750233130221264No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA57320.14208425606299696No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT52870.13105363953333302No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC48430.12004781090598296No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC45120.11184301524009811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG10500.016.2095245
ATTAGAG6900.015.5507253
CAAGACG11100.015.54
TACCGTC9600.015.2239597
TTAGAGT7800.015.1794874
CGTATAC1101.4535861E-515.1363643
ACCGTCG9450.014.8783078
TATCTAG10800.014.7314821
ATACCGT9700.014.6855676
CGTCGTA9400.014.56382810
TATAACG1406.0051207E-714.5357142
AGGTTAT6900.014.4782611
GTATTAG8200.014.4390241
AATTAAC7100.014.3309862
TAGGGTG3750.014.3066665
GGTTATC6250.014.2079992
ACTCTAA8600.014.19767410
CCGTCGT9800.013.9693899
AATTAGA7700.013.9350642
TAATACG1203.3043958E-513.8750014
Produced by FastQC (version 0.11.5)