FastQCFastQC Report
Thu 9 Feb 2017
SRR2727154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507782
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT157670.628722911321638No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG117340.46790350995421454No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC113590.45295005706237623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110320.43991064614069325No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA84920.33862592521997525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68060.2713952010182703No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG67350.26856401393741564No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT60950.24304345433534494No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG57140.22785074619723727No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG54530.21744314298451778No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC49300.1965880606847007No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG46530.18554244348192944No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC43110.17190489444457294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39810.15874585589975523No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA37090.14789961806887522No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT33480.1335044274183322No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC32860.13103212320688162No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC31220.12449247980885102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC2550.015.9607843
TCGTTAC1059.3518265E-615.85714423
AGGTTAT3150.015.8571421
GTACTAA957.064997E-515.5789481
ATACCGT6000.015.1083346
GTAGCAC1406.0025013E-714.5357143
TACCGTC5500.014.4636357
CGAACGA3500.014.27142816
ACGAACG3500.014.27142815
TATCTAG6250.014.2081
TAACGAA3650.014.19178213
GTATTAG5550.014.01
CCGATAA3700.014.09
GGTTATC3450.013.9420292
CTCGATT4400.013.87525
ATGCGGG800.00630135913.87511
CGAGACT3750.013.81333420
TAGAGTC7100.013.8098595
TTAGAGT4300.013.7674424
GTATACT1356.5739114E-613.7037034
Produced by FastQC (version 0.11.5)