FastQCFastQC Report
Thu 9 Feb 2017
SRR2727153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5330776
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT162910.3056027865361441No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG121210.2273777776443805No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC104450.19593770212817047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96780.1815495530106686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81460.1528107727655411No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA74220.13922926043037637No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT69690.13073143572342938No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG57290.1074702820002191No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG53890.10109222372127434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18250.017.2328785
CAAGACG18950.016.8891834
AGGTTAT11450.016.6419221
GGTTATC11050.016.5746612
TTATCTA12250.016.1591844
TTAGAGT9700.016.0206184
GTATACG957.067379E-515.5789481
TAGAGTG10750.015.1441875
CACTCTA11800.015.0508479
CGCAAGA22000.014.7999992
CGAACGT5150.014.7281564
GTTATCT13350.014.5505613
TCGTTAC5800.014.35344823
GCGCAAG21800.014.2568811
ACTCTAA12400.014.17338810
GGACCGT2101.364242E-1014.0952396
AGACGGA22800.014.0372816
CGAACGA9250.013.816
ACGAACG9250.013.815
GACGGAC22000.013.7909097
Produced by FastQC (version 0.11.5)