##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727153.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5330776
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.484323295520202	31.0	30.0	31.0	28.0	34.0
2	30.896733796355353	31.0	31.0	34.0	28.0	34.0
3	31.243030658200606	31.0	31.0	34.0	28.0	34.0
4	35.138233157799164	35.0	35.0	37.0	33.0	37.0
5	34.86510725642946	35.0	35.0	37.0	32.0	37.0
6	34.72176902574785	35.0	35.0	37.0	32.0	37.0
7	34.60242711380107	35.0	35.0	37.0	30.0	37.0
8	34.57821637975409	35.0	35.0	37.0	30.0	37.0
9	35.96933073158579	37.0	35.0	39.0	30.0	39.0
10	35.795284588960406	37.0	35.0	39.0	30.0	39.0
11	35.77747236049686	37.0	35.0	39.0	30.0	39.0
12	35.58949954002944	37.0	35.0	39.0	30.0	39.0
13	35.690413553298804	37.0	35.0	39.0	30.0	39.0
14	36.49129882778792	38.0	35.0	40.0	30.0	41.0
15	36.4500641182447	38.0	35.0	40.0	30.0	41.0
16	36.310769764101885	38.0	35.0	40.0	30.0	41.0
17	36.21232556010607	38.0	34.0	40.0	30.0	41.0
18	36.05995468577183	38.0	34.0	40.0	29.0	41.0
19	35.977215324748215	38.0	34.0	40.0	28.0	41.0
20	36.41234428158302	38.0	35.0	40.0	30.0	41.0
21	36.48796422884773	38.0	35.0	40.0	30.0	41.0
22	36.443802740914265	38.0	35.0	40.0	30.0	41.0
23	36.351600780074044	38.0	35.0	40.0	30.0	41.0
24	36.17439430957144	38.0	34.0	40.0	29.0	41.0
25	35.86644064578966	38.0	34.0	40.0	27.0	41.0
26	35.64891659300635	38.0	34.0	40.0	27.0	41.0
27	35.47563487942468	38.0	34.0	40.0	27.0	41.0
28	35.195149261570926	38.0	34.0	40.0	26.0	41.0
29	35.02156027565218	38.0	33.0	40.0	25.0	41.0
30	34.70659600028213	38.0	33.0	40.0	24.0	41.0
31	34.57833062203326	38.0	33.0	40.0	24.0	41.0
32	34.394649672017735	38.0	33.0	40.0	24.0	41.0
33	34.32431751024616	38.0	33.0	40.0	24.0	41.0
34	34.12490301599617	37.0	32.0	40.0	23.0	41.0
35	33.7085465605758	37.0	31.0	40.0	21.0	41.0
36	33.693460764436544	37.0	31.0	40.0	21.0	41.0
37	33.54862519077898	37.0	31.0	40.0	20.0	41.0
38	33.42772515671265	37.0	31.0	40.0	19.0	41.0
39	33.29732481724987	37.0	31.0	40.0	19.0	41.0
40	33.08967249796277	37.0	31.0	40.0	18.0	41.0
41	32.89417056728701	37.0	30.0	40.0	16.0	41.0
42	32.73563529962617	37.0	30.0	40.0	15.0	41.0
43	32.627179232441954	37.0	30.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	22.0
11	36.0
12	45.0
13	62.0
14	122.0
15	257.0
16	540.0
17	1051.0
18	2272.0
19	4333.0
20	8145.0
21	13978.0
22	23046.0
23	34635.0
24	49657.0
25	68347.0
26	89640.0
27	116288.0
28	145762.0
29	179279.0
30	217773.0
31	260246.0
32	307368.0
33	360436.0
34	419333.0
35	486726.0
36	566865.0
37	655956.0
38	773458.0
39	545094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.9231188855056	22.87899547833186	14.763085149329102	26.43480048683344
2	19.08380693542554	24.14458983082388	32.20699950626325	24.56460372748733
3	19.001792609556283	25.753567585657322	30.25435696416432	24.990282840622076
4	15.317300895779526	17.44237611934923	33.417648762581656	33.82267422228959
5	13.204812957813273	39.77972437783917	33.37000091543895	13.645461748908602
6	33.83773769522486	38.16247390623804	14.37396731732866	13.62582108120844
7	28.25933785250027	32.33825619384495	20.968316807909392	18.434089145745386
8	27.44386558354731	36.1709064496426	18.981870556932048	17.403357409878037
9	27.859865055294016	14.525371165473844	18.26709657280666	39.34766720642548
10	16.757747840089323	28.52278917741057	32.28454919133725	22.434913791162863
11	36.984390265132134	22.681669610578272	20.01344644757161	20.320493676717984
12	21.4171820387876	27.282744576024204	28.77361569872754	22.526457686460656
13	30.391016242288178	20.297345076964405	24.452424937757655	24.859213742989763
14	22.699396860794753	21.58586292127075	24.346061436458783	31.36867878147572
15	25.793074028996905	28.559219145580307	20.999175354582523	24.64853147084027
16	24.7597535518281	26.650528928621277	23.973170135079773	24.616547384470856
17	24.355703559856952	27.544263724455874	24.190005357568957	23.910027358118217
18	25.042095184641035	25.01390041524911	25.43575269341649	24.50825170669336
19	25.624937157366958	26.186900368726807	24.707753617859765	23.480408856046473
20	24.707828653839517	24.98187881088982	25.353044284734533	24.957248250536132
21	25.26988566017405	26.26589449641103	24.798565912354974	23.665653931059943
22	25.017370829312657	25.68340894458893	24.230149606736433	25.069070619361984
23	23.884177463093554	25.28052201030394	24.875271442656754	25.960029083945752
24	24.18507174190024	26.507153930309585	24.711655488806883	24.59611883898329
25	24.887858728260202	25.43413941985182	24.231068797488394	25.446933054399583
26	24.968597442473666	25.83621971735447	24.746190798487874	24.448992041683987
27	25.646660073505245	25.636155036339925	23.925522287937063	24.791662602217762
28	24.512547516534177	25.843010473522053	24.925601826075603	24.718840183868167
29	24.636188052171015	25.783600736553176	25.170200361073135	24.41001085020267
30	24.5165994594408	26.292194607314208	25.211413872952082	23.979792060292908
31	25.350305471473572	25.39041220265117	24.26774263259233	24.99153969328293
32	23.745154551607495	26.117585882430628	24.47172794354893	25.665531622412946
33	24.22666043367795	25.228878497239428	25.174477411919018	25.36998365716361
34	24.584994754985015	25.533655887998297	25.40566326553582	24.475686091480863
35	25.124428413424237	25.627094441784838	25.32798226749727	23.920494877293663
36	24.79637110994722	25.603420590172988	25.014650775046636	24.585557524833156
37	24.69355305869164	25.398140908565658	25.272849581374267	24.63545645136843
38	24.700024911945277	24.709273096449746	25.827140363804446	24.76356162780053
39	24.918473407999137	24.335912820197283	25.52134998731892	25.224263784484663
40	24.791943987141835	24.808770805601284	26.038760585700842	24.36052462155604
41	23.689008879757843	25.266771667014332	26.273604443330576	24.770615009897245
42	24.83325129399547	25.168755918462903	26.250831023475758	23.74716176406587
43	24.12971394783799	24.195032768212357	26.243439979470157	25.4318133044795
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	492.0
1	715.0
2	938.0
3	964.0
4	990.0
5	990.0
6	1285.5
7	1581.0
8	2427.0
9	3273.0
10	5351.5
11	7430.0
12	7430.0
13	15692.0
14	23954.0
15	30416.5
16	36879.0
17	31014.0
18	25149.0
19	25149.0
20	30507.5
21	35866.0
22	36070.0
23	36274.0
24	43041.0
25	49808.0
26	49808.0
27	59131.5
28	68455.0
29	78527.0
30	88599.0
31	100324.0
32	112049.0
33	112049.0
34	133441.0
35	154833.0
36	181422.5
37	208012.0
38	236043.5
39	264075.0
40	264075.0
41	283508.0
42	302941.0
43	321755.5
44	340570.0
45	375156.0
46	409742.0
47	409742.0
48	471046.5
49	532351.0
50	527268.5
51	522186.0
52	472320.5
53	422455.0
54	422455.0
55	399085.5
56	375716.0
57	358606.5
58	341497.0
59	316530.0
60	291563.0
61	291563.0
62	249648.5
63	207734.0
64	181118.5
65	154503.0
66	131278.0
67	108053.0
68	108053.0
69	87807.5
70	67562.0
71	60466.0
72	53370.0
73	42633.5
74	31897.0
75	31897.0
76	24583.0
77	17269.0
78	14805.0
79	12341.0
80	9659.5
81	6978.0
82	6978.0
83	5907.5
84	4837.0
85	3937.0
86	3037.0
87	2965.5
88	2894.0
89	2894.0
90	2073.0
91	1252.0
92	951.5
93	651.0
94	595.0
95	539.0
96	539.0
97	310.0
98	81.0
99	90.5
100	100.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5330776.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.45739171597184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.91084422963172	43.17833180742055
2	8.259746184052858	8.50049989734623
3	2.736870598658253	4.224966674132522
4	1.352265815449411	2.7833628707879368
5	0.8185510147276521	2.1060250102173512
6	0.5210252050122866	1.608635884092706
7	0.3898657650283869	1.4043032771398916
8	0.2865113138875851	1.1794499927816982
9	0.20249506916125473	0.9377881284945145
>10	1.3463952267224475	12.8504561162362
>50	0.09689942162008958	3.4324601716908827
>100	0.06297659524275165	6.403519818149841
>500	0.008400392297798332	3.089863190962625
>1k	0.006712973522323205	6.461317020362885
>5k	3.301462388023465E-4	1.1055526306167063
>10k+	1.1004874626744884E-4	0.7334675095677468
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16291	0.3056027865361441	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG	12121	0.2273777776443805	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	10445	0.19593770212817047	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9678	0.1815495530106686	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8146	0.1528107727655411	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA	7422	0.13922926043037637	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	6969	0.13073143572342938	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG	5729	0.1074702820002191	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	5389	0.10109222372127434	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.8758994938072805E-5	0.0
3	0.0	0.0	0.0	1.8758994938072805E-5	0.0
4	0.0	0.0	0.0	3.751798987614561E-5	0.0
5	0.0	0.0	0.0	3.751798987614561E-5	0.0
6	0.0	0.0	0.0	3.751798987614561E-5	5.6276984814218416E-5
7	0.0	0.0	0.0	3.751798987614561E-5	5.6276984814218416E-5
8	0.0	0.0	0.0	3.751798987614561E-5	5.6276984814218416E-5
9	0.0	0.0	0.0	3.751798987614561E-5	1.1255396962843683E-4
10	0.0	0.0	0.0	3.751798987614561E-5	1.1255396962843683E-4
11	0.0	0.0	0.0	3.751798987614561E-5	1.1255396962843683E-4
12	0.0	0.0	0.0	3.751798987614561E-5	1.1255396962843683E-4
13	0.0	0.0	0.0	7.503597975229122E-5	1.1255396962843683E-4
14	0.0	0.0	0.0	1.3131296456650964E-4	1.3131296456650964E-4
15	0.0	0.0	0.0	2.0634894431880086E-4	1.3131296456650964E-4
16	0.0	0.0	0.0	4.314568835756745E-4	1.6883095444265525E-4
17	0.0	0.0	0.0	6.378058278944754E-4	1.8758994938072805E-4
18	0.0	0.0	0.0	8.441547722132762E-4	1.8758994938072805E-4
19	0.0	0.0	0.0	0.0012380936659128052	1.8758994938072805E-4
20	0.0	0.0	0.0	0.0016507915545504069	1.8758994938072805E-4
21	0.0	0.0	0.0	0.002870126225525139	2.0634894431880086E-4
22	0.0	0.0	0.0	0.004483399790199401	2.0634894431880086E-4
23	0.0	0.0	0.0	0.007972572848680942	3.751798987614561E-4
24	0.0	0.0	0.0	0.014012969218740385	3.939388936995289E-4
25	0.0	0.0	0.0	0.018214984084868695	3.939388936995289E-4
26	0.0	0.0	0.0	0.023354948697900644	3.939388936995289E-4
27	0.0	0.0	0.0	0.03417888877716865	4.126978886376017E-4
28	0.0	0.0	0.0	0.0568397546623606	4.126978886376017E-4
29	0.0	0.0	0.0	0.08572860686699273	4.126978886376017E-4
30	0.0	0.0	0.0	0.1285178743207368	4.126978886376017E-4
31	0.0	0.0	0.0	0.26176301536586793	4.126978886376017E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	1825	0.0	17.232878	5
CAAGACG	1895	0.0	16.889183	4
AGGTTAT	1145	0.0	16.641922	1
GGTTATC	1105	0.0	16.574661	2
TTATCTA	1225	0.0	16.159184	4
TTAGAGT	970	0.0	16.020618	4
GTATACG	95	7.067379E-5	15.578948	1
TAGAGTG	1075	0.0	15.144187	5
CACTCTA	1180	0.0	15.050847	9
CGCAAGA	2200	0.0	14.799999	2
CGAACGT	515	0.0	14.728156	4
GTTATCT	1335	0.0	14.550561	3
TCGTTAC	580	0.0	14.353448	23
GCGCAAG	2180	0.0	14.256881	1
ACTCTAA	1240	0.0	14.173388	10
GGACCGT	210	1.364242E-10	14.095239	6
AGACGGA	2280	0.0	14.037281	6
CGAACGA	925	0.0	13.8	16
ACGAACG	925	0.0	13.8	15
GACGGAC	2200	0.0	13.790909	7
>>END_MODULE