FastQCFastQC Report
Thu 9 Feb 2017
SRR2727152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433398
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91780.3771680588214505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT91630.3765516368469112No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66400.27286946072939977No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44820.18418688599234487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC18800.016.13829837
GGTATCA46550.015.8968861
GTACGAT700.00259360915.8571436
GCGTTAA750.00410615914.81
TCTTATA29150.014.02572937
TCGTTAC3500.013.21428523
CTCGTTA4200.013.21428522
TACTTAC6950.013.04316631
TATACGG1000.001833503112.9500012
CTAGCGT1000.001833503112.9500014
GTATAGG3350.012.7014931
GGACCGT2052.0683729E-812.6341466
GTCTTAT2358.094503E-1012.5957451
GTAATAC2102.8951035E-812.3333333
GGCGTCA4700.012.20212812
CTCTTAT44350.012.13867137
GTGCTAC2901.2732926E-1112.1206891
GGAGTGT9800.012.0816336
TATACAC7450.011.91946337
TACACCG1401.3995358E-411.8928585
Produced by FastQC (version 0.11.5)