##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727152.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2433398
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38719395676334	31.0	31.0	34.0	30.0	34.0
2	31.639294928326564	31.0	31.0	34.0	30.0	34.0
3	31.747701773404927	31.0	31.0	34.0	30.0	34.0
4	35.51445879383479	37.0	35.0	37.0	33.0	37.0
5	35.06710698373222	37.0	35.0	37.0	32.0	37.0
6	35.07965158186207	36.0	35.0	37.0	32.0	37.0
7	35.03160724221849	36.0	35.0	37.0	32.0	37.0
8	34.97868988139219	36.0	35.0	37.0	32.0	37.0
9	36.79316453781913	39.0	37.0	39.0	33.0	39.0
10	36.528055829749185	38.0	35.0	39.0	32.0	39.0
11	36.66316360907669	38.0	35.0	39.0	32.0	39.0
12	36.523434719680054	38.0	35.0	39.0	32.0	39.0
13	36.67283650270116	38.0	35.0	39.0	32.0	39.0
14	37.65148528929505	39.0	37.0	40.0	32.0	41.0
15	37.657362667348295	39.0	37.0	40.0	32.0	41.0
16	37.590434035040715	39.0	37.0	40.0	32.0	41.0
17	37.50429933779842	39.0	36.0	40.0	32.0	41.0
18	37.51788157958542	39.0	36.0	40.0	32.0	41.0
19	37.56488293324808	39.0	37.0	40.0	32.0	41.0
20	37.435373909241314	39.0	36.0	40.0	32.0	41.0
21	37.42902024247575	39.0	36.0	40.0	32.0	41.0
22	37.32254526386559	39.0	36.0	40.0	32.0	41.0
23	37.25809094936381	39.0	36.0	40.0	32.0	41.0
24	37.14118159051664	39.0	36.0	40.0	31.0	41.0
25	37.11634841485034	39.0	36.0	40.0	31.0	41.0
26	36.956231985067795	39.0	36.0	40.0	31.0	41.0
27	36.75321217490932	39.0	36.0	40.0	30.0	41.0
28	36.62975600374456	39.0	35.0	40.0	30.0	41.0
29	36.5354348117324	38.0	35.0	40.0	30.0	41.0
30	36.4778979846289	38.0	35.0	40.0	30.0	41.0
31	36.42782520574111	38.0	35.0	40.0	30.0	41.0
32	36.315246416739065	38.0	35.0	40.0	30.0	41.0
33	36.28229907314792	38.0	35.0	40.0	30.0	41.0
34	36.26952804267941	38.0	35.0	40.0	30.0	41.0
35	36.163517435290075	38.0	35.0	40.0	30.0	41.0
36	36.070368266925506	38.0	35.0	40.0	29.0	41.0
37	35.920684984536024	38.0	35.0	40.0	29.0	41.0
38	35.87138560975229	38.0	35.0	40.0	29.0	41.0
39	35.75487404855268	38.0	34.0	40.0	28.0	41.0
40	35.67984768623957	38.0	34.0	40.0	28.0	41.0
41	35.610358437049754	38.0	34.0	40.0	27.0	41.0
42	35.54399157063497	38.0	34.0	40.0	27.0	41.0
43	34.19639820530797	37.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	2.0
12	3.0
13	2.0
14	5.0
15	7.0
16	15.0
17	42.0
18	99.0
19	225.0
20	493.0
21	946.0
22	1915.0
23	3551.0
24	5997.0
25	9712.0
26	15116.0
27	22572.0
28	32271.0
29	44643.0
30	60083.0
31	79359.0
32	101337.0
33	129405.0
34	167289.0
35	217786.0
36	293299.0
37	403998.0
38	557690.0
39	285527.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.1400034026493	18.863868549246774	13.018256775093922	25.977871273010006
2	18.534082792868244	21.093836684340168	35.52000124928187	24.852079273509716
3	20.042590648960836	22.814763552859006	30.050406879597997	27.09223891858216
4	15.32996246401123	15.810196276975653	33.88672958554252	34.9731116734706
5	15.538313091405517	35.647148555230174	33.6762420286365	15.13829632472781
6	35.25752877252303	34.10502515412604	15.141214055407296	15.496232017943631
7	28.993571951649503	30.411054829501794	21.366007533498426	19.22936568535028
8	26.259041882996538	35.03795926519213	19.693531432178375	19.00946741963296
9	26.75189179903986	14.862714607310437	19.67306622262367	38.71232737102603
10	17.27929422149603	26.55254093247385	33.0019174832888	23.16624736274132
11	35.5001935565	21.87595288563564	21.400034026492996	21.22381953137136
12	21.30617350716981	24.882859277438378	30.3186737229175	23.49229349247431
13	30.802112930149526	19.918730926876737	24.41392653400718	24.86522960896656
14	22.159137140739	20.769023398556257	25.90328421409075	31.168555246613995
15	25.21124781067462	27.532816251184556	22.820064781840045	24.435871156300777
16	24.17508356627235	26.217659421105793	25.01851320663533	24.588743805986528
17	23.91852874046909	26.24137111972641	25.69209804561358	24.14800209419092
18	23.840325339299202	25.504541386160422	26.567992576635636	24.08714069790474
19	24.93192646661171	25.253739832119532	26.84053327897861	22.973800422290147
20	24.298573435171722	25.05973128933286	26.72801572122604	23.91367955426938
21	23.908419420086645	25.573375173317313	27.05841789957911	23.459787507016937
22	24.549785937195644	25.022992539650318	26.22427568363252	24.202945839521526
23	23.873201177941297	25.387133547409835	26.57945802536207	24.1602072492868
24	23.76405339364954	25.360134264925016	26.57752656984184	24.298285771583604
25	24.29097089748574	25.081141679248525	26.359641949241347	24.26824547402439
26	23.809339861379026	25.373325695180153	26.65601763459985	24.161316808840972
27	24.218520768078218	25.75600045697416	26.297095666224763	23.728383108722863
28	23.90439212985299	25.776630045722072	26.172619522166123	24.146358302258815
29	23.83962672772806	26.01748665857373	26.58089634330266	23.56199027039555
30	24.28649156447075	25.714165952302086	26.182358989363845	23.816983493863315
31	24.083442166057505	25.161235441140335	26.669907676426135	24.08541471637603
32	23.508525937803846	25.737631082132882	26.516582983959058	24.237259996104214
33	23.61652306774313	25.134195063857206	26.610566787677147	24.638715080722513
34	24.012060501405852	25.18913880918781	26.998172925267465	23.80062776413887
35	23.76787520989168	25.228261057171906	26.800630229826766	24.203233503109644
36	23.568318869334156	24.8658871257394	27.243180112747687	24.322613892178758
37	23.25768328896465	24.530224813203596	27.62223853229106	24.589853365540694
38	23.376899298840552	24.38931074982391	27.818836047370798	24.414953903964744
39	22.840283422604934	24.44310384080204	28.444997489107827	24.271615247485204
40	23.262779043954176	24.194439216272883	28.453750681146282	24.089031058626663
41	22.615125022704877	24.38663958793424	28.564213498983726	24.43402189037716
42	22.22731341112305	24.741205507689248	28.520159875203316	24.511321205984387
43	22.07008471281722	24.37768092190427	28.28279632020738	25.26943804507113
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1492.0
1	1319.0
2	1146.0
3	1993.0
4	2840.0
5	2840.0
6	3571.5
7	4303.0
8	4018.0
9	3733.0
10	5222.0
11	6711.0
12	6711.0
13	10534.0
14	14357.0
15	18245.5
16	22134.0
17	20258.5
18	18383.0
19	18383.0
20	20710.5
21	23038.0
22	20039.0
23	17040.0
24	19867.0
25	22694.0
26	22694.0
27	27119.5
28	31545.0
29	35951.0
30	40357.0
31	46724.5
32	53092.0
33	53092.0
34	61263.5
35	69435.0
36	78869.5
37	88304.0
38	99024.5
39	109745.0
40	109745.0
41	122155.0
42	134565.0
43	147556.5
44	160548.0
45	173510.0
46	186472.0
47	186472.0
48	198910.0
49	211348.0
50	215853.5
51	220359.0
52	216724.0
53	213089.0
54	213089.0
55	201977.5
56	190866.0
57	177244.5
58	163623.0
59	148884.0
60	134145.0
61	134145.0
62	117016.5
63	99888.0
64	84474.5
65	69061.0
66	57260.0
67	45459.0
68	45459.0
69	37057.5
70	28656.0
71	23307.0
72	17958.0
73	14606.0
74	11254.0
75	11254.0
76	9155.0
77	7056.0
78	5579.0
79	4102.0
80	3170.5
81	2239.0
82	2239.0
83	1776.0
84	1313.0
85	1001.0
86	689.0
87	475.0
88	261.0
89	261.0
90	168.5
91	76.0
92	46.5
93	17.0
94	9.5
95	2.0
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2433398.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.58808637744988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.71747899374022	54.071517645500386
2	8.765047691484003	11.322353148000731
3	2.840954913230866	5.504755239905875
4	1.3383776877576883	3.4577301481018083
5	0.7897059039566627	2.5502796568767536
6	0.5446635624131849	2.110726632947541
7	0.36329394310065893	1.6425122404172807
8	0.29176405708156883	1.507558569649567
9	0.2036705208116892	1.1839220271653028
>10	1.0934945332006747	12.16662734508115
>50	0.038645654677679095	1.6700657571293698
>100	0.01213815907129077	1.2452418905165106
>500	1.9109486843563948E-4	0.0785391353917452
>1k	3.8218973686806885E-4	0.46041459620891945
>5k	1.9109486843403443E-4	1.0277559671070517
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9178	0.3771680588214505	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9163	0.3765516368469112	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6640	0.27286946072939977	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4482	0.18418688599234487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.109479830262045E-5	0.0	0.0	0.0
6	0.0	4.109479830262045E-5	0.0	0.0	8.21895966052409E-5
7	0.0	4.109479830262045E-5	0.0	0.0	8.21895966052409E-5
8	0.0	4.109479830262045E-5	0.0	0.0	8.21895966052409E-5
9	0.0	4.109479830262045E-5	0.0	4.109479830262045E-5	1.2328439490786136E-4
10	4.109479830262045E-5	4.109479830262045E-5	0.0	4.109479830262045E-5	1.2328439490786136E-4
11	8.21895966052409E-5	4.109479830262045E-5	0.0	8.21895966052409E-5	1.2328439490786136E-4
12	8.21895966052409E-5	4.109479830262045E-5	0.0	1.2328439490786136E-4	1.2328439490786136E-4
13	8.21895966052409E-5	4.109479830262045E-5	0.0	1.2328439490786136E-4	1.2328439490786136E-4
14	1.2328439490786136E-4	4.109479830262045E-5	0.0	1.2328439490786136E-4	2.8766358811834316E-4
15	1.2328439490786136E-4	4.109479830262045E-5	0.0	1.2328439490786136E-4	3.287583864209636E-4
16	1.2328439490786136E-4	4.109479830262045E-5	0.0	2.465687898157227E-4	3.6985318472358404E-4
17	1.2328439490786136E-4	4.109479830262045E-5	0.0	5.342323779340658E-4	3.6985318472358404E-4
18	1.2328439490786136E-4	4.109479830262045E-5	0.0	5.342323779340658E-4	3.6985318472358404E-4
19	1.2328439490786136E-4	4.109479830262045E-5	0.0	7.397063694471681E-4	3.6985318472358404E-4
20	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.0010273699575655112	3.6985318472358404E-4
21	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.0018492659236179203	3.6985318472358404E-4
22	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.002630067091367709	3.6985318472358404E-4
23	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.003451963057420118	5.753271762366863E-4
24	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.005342323779340658	5.753271762366863E-4
25	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.006821736518234995	5.753271762366863E-4
26	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.009862751592628909	5.753271762366863E-4
27	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.0323416062641623	5.753271762366863E-4
28	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.09357285573506677	5.753271762366863E-4
29	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.19220037166135584	5.753271762366863E-4
30	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.30907397803400843	5.753271762366863E-4
31	1.2328439490786136E-4	4.109479830262045E-5	0.0	0.651147079105021	5.753271762366863E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1880	0.0	16.138298	37
GGTATCA	4655	0.0	15.896886	1
GTACGAT	70	0.002593609	15.857143	6
GCGTTAA	75	0.004106159	14.8	1
TCTTATA	2915	0.0	14.025729	37
TCGTTAC	350	0.0	13.214285	23
CTCGTTA	420	0.0	13.214285	22
TACTTAC	695	0.0	13.043166	31
TATACGG	100	0.0018335031	12.950001	2
CTAGCGT	100	0.0018335031	12.950001	4
GTATAGG	335	0.0	12.701493	1
GGACCGT	205	2.0683729E-8	12.634146	6
GTCTTAT	235	8.094503E-10	12.595745	1
GTAATAC	210	2.8951035E-8	12.333333	3
GGCGTCA	470	0.0	12.202128	12
CTCTTAT	4435	0.0	12.138671	37
GTGCTAC	290	1.2732926E-11	12.120689	1
GGAGTGT	980	0.0	12.081633	6
TATACAC	745	0.0	11.919463	37
TACACCG	140	1.3995358E-4	11.892858	5
>>END_MODULE