FastQCFastQC Report
Thu 9 Feb 2017
SRR2727151.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727151.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3274592
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105670.3226966901525442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT95060.2902957070682393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74580.22775356441352082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54890.16762393605065914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43500.016.756321
CTTATAC28650.015.82024537
TCTTATA45850.013.75899637
GTATACG1000.001833738912.9499991
CTCTTAT69000.012.62826137
GGACCGT2451.5643309E-912.0816336
TCTATAC3250.011.9538463
GGGTAAG13650.011.926741
GTATTAG2951.6370905E-1111.9152551
CATTACG4850.011.82474124
GTTACGG5500.011.77272825
TATACCG1100.00374758211.7727275
TACGGAT8350.011.74251527
ATTACGG5550.011.66666725
GTATCAA62200.011.6591642
GTAAGAG14850.011.5858583
ACCCGAC13600.011.290441511
TCGTTAC4950.011.21212123
GACCGTG4300.011.1860487
CGTTACG4000.011.09999924
Produced by FastQC (version 0.11.5)