##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727151.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3274592
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.594545213571646	31.0	31.0	34.0	30.0	34.0
2	31.844793183395062	31.0	31.0	34.0	30.0	34.0
3	31.96047141140026	33.0	31.0	34.0	30.0	34.0
4	35.690059708201815	37.0	35.0	37.0	33.0	37.0
5	35.29153555618532	37.0	35.0	37.0	33.0	37.0
6	35.30200403592264	37.0	35.0	37.0	32.0	37.0
7	35.25561871524758	37.0	35.0	37.0	32.0	37.0
8	35.20193569153042	37.0	35.0	37.0	32.0	37.0
9	37.03024193548387	39.0	37.0	39.0	33.0	39.0
10	36.797281310160166	39.0	37.0	39.0	32.0	39.0
11	36.91298060949273	39.0	37.0	39.0	33.0	39.0
12	36.79117154137065	39.0	37.0	39.0	32.0	39.0
13	36.92621981608701	39.0	37.0	39.0	33.0	39.0
14	37.96726767792751	40.0	37.0	41.0	33.0	41.0
15	37.96757733482522	40.0	37.0	41.0	33.0	41.0
16	37.91232495529214	39.0	37.0	41.0	33.0	41.0
17	37.825954500591216	39.0	37.0	41.0	33.0	41.0
18	37.83821587544342	39.0	37.0	41.0	33.0	41.0
19	37.88169182603512	40.0	37.0	41.0	33.0	41.0
20	37.75931535898213	39.0	37.0	41.0	32.0	41.0
21	37.75085109839638	39.0	37.0	41.0	32.0	41.0
22	37.65552380265999	39.0	37.0	40.0	32.0	41.0
23	37.594174480362746	39.0	37.0	40.0	32.0	41.0
24	37.489975239663444	39.0	36.0	40.0	32.0	41.0
25	37.46408071600981	39.0	36.0	40.0	32.0	41.0
26	37.330618593094954	39.0	36.0	40.0	32.0	41.0
27	37.13488703325483	39.0	36.0	40.0	31.0	41.0
28	37.02726538145821	39.0	36.0	40.0	31.0	41.0
29	36.93906630200037	39.0	36.0	40.0	31.0	41.0
30	36.89621058134876	39.0	36.0	40.0	31.0	41.0
31	36.85259049066266	39.0	36.0	40.0	30.0	41.0
32	36.747913633209876	39.0	35.0	40.0	30.0	41.0
33	36.724552554944246	39.0	35.0	40.0	30.0	41.0
34	36.71147336828527	39.0	35.0	40.0	30.0	41.0
35	36.61416506239556	39.0	35.0	40.0	30.0	41.0
36	36.545812119494585	39.0	35.0	40.0	30.0	41.0
37	36.41900700911747	38.0	35.0	40.0	30.0	41.0
38	36.37063884599974	38.0	35.0	40.0	30.0	41.0
39	36.26864537627894	38.0	35.0	40.0	30.0	41.0
40	36.20427247119641	38.0	35.0	40.0	30.0	41.0
41	36.154617430201995	38.0	35.0	40.0	30.0	41.0
42	36.10184413813996	38.0	35.0	40.0	29.0	41.0
43	34.747441818705965	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	3.0
12	0.0
13	2.0
14	4.0
15	4.0
16	7.0
17	31.0
18	69.0
19	194.0
20	391.0
21	863.0
22	1755.0
23	3353.0
24	5898.0
25	9669.0
26	15595.0
27	23498.0
28	34607.0
29	49633.0
30	68261.0
31	90884.0
32	119807.0
33	155171.0
34	205061.0
35	272768.0
36	375993.0
37	533342.0
38	792516.0
39	515211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2914366125612	18.55654078431756	12.853967761479904	26.29805484164134
2	18.3906880612913	20.667979400181764	35.81820269517546	25.123129843351478
3	20.00640079741232	22.4226407442515	29.88882279074767	27.682135667588515
4	15.173004759066167	15.60270714641702	33.77507793337307	35.44921016114374
5	15.617304384790534	35.32925628597395	33.74295179368911	15.310487535546413
6	35.40737288798116	33.81126564775093	15.231729632271746	15.54963183199617
7	29.10274012762506	30.08133532360673	21.383793767284594	19.432130781483618
8	26.155288964243482	34.96933358415338	19.750338362763973	19.12503908883916
9	26.610368558892223	14.985347792946419	19.70856216591258	38.69572148224878
10	17.3806996413599	26.392326127957315	33.01098274227751	23.215991488405272
11	35.622025583645225	21.717667422384224	21.438273836862727	21.222033157107816
12	21.37454070613988	24.607981696651063	30.388427016251185	23.629050580957873
13	30.798646060333624	19.764446990647997	24.418767284596065	25.018139664422318
14	22.266193773147922	20.600917610499263	25.770141745903004	31.362746870449815
15	25.298846390634317	27.295186698068036	22.77340199939412	24.63256491190353
16	24.400261162306634	26.09946521581925	24.885084920503072	24.61518870137104
17	24.043636581290126	26.150280706726214	25.600319062649636	24.205763649334024
18	24.088863589723545	25.37476424543882	26.437644750857515	24.09872741398012
19	25.039638525959873	25.202009899248516	26.58034955194418	23.178002022847426
20	24.503357975589022	24.898094174785747	26.508248966588816	24.09029888303642
21	24.08587085047542	25.452514389578916	26.822578202108843	23.639036557836825
22	24.766505262334974	24.90695023990775	26.025318574039147	24.301225923718132
23	24.125051304101397	25.211995876127467	26.281411546843085	24.381541272928047
24	24.005372272331943	25.13198590847348	26.408847270133194	24.45379454906138
25	24.47446888039792	24.859096950093324	26.13519485786321	24.531239311645543
26	24.060585257644313	25.23300612717554	26.394891333027136	24.311517282153012
27	24.45254248468206	25.598517311469642	26.022936597902884	23.92600360594541
28	24.13366306397866	25.569353372878208	25.902310883310044	24.39467267983309
29	23.992729475916388	25.750963784190517	26.399075060343396	23.857231679549695
30	24.45486338450714	25.4188308039597	26.064773871065466	24.0615319404677
31	24.28204796200565	24.934129198385634	26.45089831038493	24.332924529223792
32	23.750928359930032	25.501497591150287	26.316835807331113	24.43073824158857
33	23.85961365568596	24.894643363203723	26.38591311528276	24.85982986582756
34	24.225918832025485	24.876228855381065	26.843221995289795	24.05463031730365
35	24.010685911405147	24.77166621063021	26.681888919291318	24.535758958673327
36	23.715504099442004	24.50903807252934	27.120294680986213	24.65516314704244
37	23.44734855517878	24.1832264905063	27.549050385513674	24.820374568801242
38	23.490285201942715	23.929271188593876	27.87446497151401	24.7059786379494
39	22.931833950611253	23.980086679500836	28.49341230907545	24.594667060812462
40	23.353932337219415	23.78522270866111	28.47933421934702	24.381510734772455
41	22.67641281723036	23.997982038678405	28.57061276641487	24.754992377676363
42	22.288211783330564	24.356286218252535	28.596967194691736	24.758534803725166
43	22.05722117442417	24.04778977045079	28.433710214890894	25.46127884023414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2655.0
1	2302.0
2	1949.0
3	3248.0
4	4547.0
5	4547.0
6	5199.5
7	5852.0
8	5227.5
9	4603.0
10	6404.0
11	8205.0
12	8205.0
13	12713.5
14	17222.0
15	20403.5
16	23585.0
17	22058.5
18	20532.0
19	20532.0
20	23504.5
21	26477.0
22	23728.0
23	20979.0
24	24776.5
25	28574.0
26	28574.0
27	34140.5
28	39707.0
29	45938.5
30	52170.0
31	60240.0
32	68310.0
33	68310.0
34	79384.0
35	90458.0
36	103506.5
37	116555.0
38	132256.0
39	147957.0
40	147957.0
41	164272.5
42	180588.0
43	198067.0
44	215546.0
45	234595.0
46	253644.0
47	253644.0
48	269877.5
49	286111.0
50	293770.0
51	301429.0
52	296800.0
53	292171.0
54	292171.0
55	276847.5
56	261524.0
57	243004.5
58	224485.0
59	204006.5
60	183528.0
61	183528.0
62	160138.0
63	136748.0
64	115748.0
65	94748.0
66	78557.0
67	62366.0
68	62366.0
69	50865.5
70	39365.0
71	32127.5
72	24890.0
73	20225.0
74	15560.0
75	15560.0
76	12538.5
77	9517.0
78	7662.5
79	5808.0
80	4468.0
81	3128.0
82	3128.0
83	2432.5
84	1737.0
85	1316.5
86	896.0
87	623.5
88	351.0
89	351.0
90	217.0
91	83.0
92	53.5
93	24.0
94	15.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3274592.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.22256828927485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.98587670058491	52.465802567480715
2	9.076892156293347	11.477288684112787
3	2.9725720424457713	5.638009168449509
4	1.4409085840929077	3.6439176542566476
5	0.847055657822866	2.6776517085761387
6	0.557683153050963	2.1154896736525575
7	0.39408018119271176	1.7440332816834214
8	0.28303168888683233	1.431519222294124
9	0.22118570490458414	1.2585535499647318
>10	1.1611364129358088	12.819428751346107
>50	0.04570979737422162	1.942671074356426
>100	0.012995737120243211	1.2972691466214832
>500	3.8763702104857274E-4	0.1491888463290999
>1k	2.90727764284218E-4	0.32763340947091585
>5k	1.45363882142109E-4	0.6878310371996703
>10k+	4.8454627380703006E-5	0.3237122242056258
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10567	0.3226966901525442	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9506	0.2902957070682393	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7458	0.22775356441352082	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5489	0.16762393605065914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.053815559312427E-5
2	0.0	0.0	0.0	0.0	3.053815559312427E-5
3	0.0	0.0	0.0	0.0	3.053815559312427E-5
4	0.0	0.0	0.0	6.107631118624855E-5	6.107631118624855E-5
5	0.0	0.0	0.0	6.107631118624855E-5	6.107631118624855E-5
6	0.0	0.0	0.0	6.107631118624855E-5	9.161446677937282E-5
7	0.0	0.0	0.0	6.107631118624855E-5	9.161446677937282E-5
8	0.0	0.0	0.0	6.107631118624855E-5	1.221526223724971E-4
9	0.0	0.0	0.0	6.107631118624855E-5	1.221526223724971E-4
10	0.0	0.0	0.0	6.107631118624855E-5	1.221526223724971E-4
11	0.0	0.0	0.0	6.107631118624855E-5	1.221526223724971E-4
12	0.0	0.0	0.0	6.107631118624855E-5	1.221526223724971E-4
13	0.0	3.053815559312427E-5	0.0	6.107631118624855E-5	1.221526223724971E-4
14	0.0	3.053815559312427E-5	0.0	6.107631118624855E-5	1.8322893355874565E-4
15	0.0	3.053815559312427E-5	0.0	9.161446677937282E-5	2.137670891518699E-4
16	0.0	3.053815559312427E-5	0.0	1.5269077796562137E-4	3.35919711524367E-4
17	0.0	3.053815559312427E-5	0.0	2.443052447449942E-4	3.664578671174913E-4
18	0.0	3.053815559312427E-5	0.0	3.0538155593124274E-4	4.275341783037398E-4
19	0.0	3.053815559312427E-5	0.0	3.9699602271061555E-4	4.275341783037398E-4
20	3.053815559312427E-5	3.053815559312427E-5	0.0	6.413012674556098E-4	4.275341783037398E-4
21	3.053815559312427E-5	3.053815559312427E-5	0.0	0.0010688354457593495	4.580723338968641E-4
22	6.107631118624855E-5	3.053815559312427E-5	0.0	0.0019544419579599535	4.580723338968641E-4
23	6.107631118624855E-5	3.053815559312427E-5	0.0	0.002595743225415563	6.71839423048734E-4
24	6.107631118624855E-5	3.053815559312427E-5	0.0	0.004183727316258026	6.71839423048734E-4
25	6.107631118624855E-5	3.053815559312427E-5	0.0	0.005924402185066109	6.71839423048734E-4
26	6.107631118624855E-5	3.053815559312427E-5	0.0	0.01007759134573101	7.023775786418583E-4
27	6.107631118624855E-5	3.053815559312427E-5	0.0	0.03881399575886095	7.023775786418583E-4
28	6.107631118624855E-5	3.053815559312427E-5	0.0	0.1176329753447147	7.023775786418583E-4
29	6.107631118624855E-5	3.053815559312427E-5	0.0	0.22405844758675278	7.329157342349826E-4
30	6.107631118624855E-5	3.053815559312427E-5	0.0	0.35399829963549656	7.329157342349826E-4
31	6.107631118624855E-5	3.053815559312427E-5	0.0	0.7284266253627932	7.329157342349826E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4350	0.0	16.75632	1
CTTATAC	2865	0.0	15.820245	37
TCTTATA	4585	0.0	13.758996	37
GTATACG	100	0.0018337389	12.949999	1
CTCTTAT	6900	0.0	12.628261	37
GGACCGT	245	1.5643309E-9	12.081633	6
TCTATAC	325	0.0	11.953846	3
GGGTAAG	1365	0.0	11.92674	1
GTATTAG	295	1.6370905E-11	11.915255	1
CATTACG	485	0.0	11.824741	24
GTTACGG	550	0.0	11.772728	25
TATACCG	110	0.003747582	11.772727	5
TACGGAT	835	0.0	11.742515	27
ATTACGG	555	0.0	11.666667	25
GTATCAA	6220	0.0	11.659164	2
GTAAGAG	1485	0.0	11.585858	3
ACCCGAC	1360	0.0	11.2904415	11
TCGTTAC	495	0.0	11.212121	23
GACCGTG	430	0.0	11.186048	7
CGTTACG	400	0.0	11.099999	24
>>END_MODULE