FastQCFastQC Report
Thu 9 Feb 2017
SRR2727150.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727150.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4138279
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144400.3489373239455339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT136130.32895317111291916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104560.2526654196104226No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73040.17649849128103737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60400.017.5811271
CTTATAC35500.015.5816937
TCTTATA55500.013.76666637
TTAGTAC2202.8558134E-1013.4545453
CTCTTAT83800.012.42899737
GTATTAC3450.012.3333331
GTATCAA86150.012.2832272
GGACCGT2205.5351848E-811.7727276
GTACCGA1901.4362686E-611.6842116
ACCGTGC4000.011.5624998
CATTACG5150.011.49514624
ACGGATG8700.011.48275928
GTAAGAG17250.011.2608693
GGGTAAG17150.011.1107871
TACGGAT8800.010.93181827
TAAGAGT6150.010.8292684
ATTACGG5500.010.76363725
TAAGGCG2759.57516E-910.7636375
TATACCG2259.377454E-710.6888895
TATACAC15800.010.65506337
Produced by FastQC (version 0.11.5)