##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727150.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4138279
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62414641448776	31.0	31.0	34.0	30.0	34.0
2	31.86838804246886	31.0	31.0	34.0	30.0	34.0
3	31.981459200793374	33.0	31.0	34.0	30.0	34.0
4	35.71113402455465	37.0	35.0	37.0	33.0	37.0
5	35.31519117004919	37.0	35.0	37.0	33.0	37.0
6	35.324223185531956	37.0	35.0	37.0	33.0	37.0
7	35.278723353355346	37.0	35.0	37.0	32.0	37.0
8	35.228837639994794	37.0	35.0	37.0	32.0	37.0
9	37.06601222392207	39.0	37.0	39.0	33.0	39.0
10	36.826129171087786	39.0	37.0	39.0	33.0	39.0
11	36.94681436413543	39.0	37.0	39.0	33.0	39.0
12	36.82523507960676	39.0	37.0	39.0	33.0	39.0
13	36.957096416167204	39.0	37.0	39.0	33.0	39.0
14	37.998768570219646	40.0	37.0	41.0	33.0	41.0
15	38.00221807181198	40.0	37.0	41.0	33.0	41.0
16	37.95108715482934	40.0	37.0	41.0	33.0	41.0
17	37.86039051499428	39.0	37.0	41.0	33.0	41.0
18	37.88017265148145	39.0	37.0	41.0	33.0	41.0
19	37.923507574042254	40.0	37.0	41.0	33.0	41.0
20	37.79897851256525	39.0	37.0	41.0	33.0	41.0
21	37.78650303664881	39.0	37.0	41.0	33.0	41.0
22	37.69347160981654	39.0	37.0	41.0	32.0	41.0
23	37.63621229984735	39.0	37.0	40.0	32.0	41.0
24	37.53394708283323	39.0	37.0	41.0	32.0	41.0
25	37.50662316388044	39.0	36.0	40.0	32.0	41.0
26	37.369780287892624	39.0	36.0	40.0	32.0	41.0
27	37.18608460183569	39.0	36.0	40.0	31.0	41.0
28	37.080202180664955	39.0	36.0	40.0	31.0	41.0
29	36.99566196479261	39.0	36.0	40.0	31.0	41.0
30	36.93727054169136	39.0	36.0	40.0	31.0	41.0
31	36.9012611281163	39.0	36.0	40.0	31.0	41.0
32	36.795380640116335	39.0	35.0	40.0	30.0	41.0
33	36.764831467380525	39.0	35.0	40.0	30.0	41.0
34	36.75186061645433	39.0	35.0	40.0	30.0	41.0
35	36.65491645198402	39.0	35.0	40.0	30.0	41.0
36	36.574781690649665	39.0	35.0	40.0	30.0	41.0
37	36.44481147839476	38.0	35.0	40.0	30.0	41.0
38	36.390648866352414	38.0	35.0	40.0	30.0	41.0
39	36.288198548237084	38.0	35.0	40.0	30.0	41.0
40	36.21910485010798	38.0	35.0	40.0	30.0	41.0
41	36.16064334956633	38.0	35.0	40.0	30.0	41.0
42	36.100497573991504	38.0	35.0	40.0	29.0	41.0
43	34.74702793117622	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	4.0
12	5.0
13	8.0
14	4.0
15	9.0
16	19.0
17	45.0
18	82.0
19	215.0
20	491.0
21	1095.0
22	2269.0
23	4032.0
24	7181.0
25	11973.0
26	18755.0
27	29157.0
28	42486.0
29	61233.0
30	83639.0
31	112328.0
32	148325.0
33	194053.0
34	257262.0
35	345378.0
36	475597.0
37	675449.0
38	1004930.0
39	662249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.434403286970266	18.61215737266627	12.74135939118653	26.212079949176943
2	18.384719831601494	20.75476303071881	35.75807721035725	25.102439927322447
3	19.88152562937395	22.58624901800966	29.963107852322185	27.569117500294205
4	15.023515814182659	15.676492570945555	33.96805773607821	35.33193387879358
5	15.525801909441098	35.40585349610309	33.8351039163865	15.233240678069313
6	35.146977765394745	34.07636362845521	15.306048722186203	15.47060988396384
7	29.092431902247284	30.177883124844897	21.46824803257586	19.261436940331958
8	26.236800370395517	34.85910447313968	19.746517815739345	19.157577340725457
9	26.61011981067492	14.904504988667993	19.813888817066225	38.67148638359086
10	17.309949377506932	26.433307179143796	33.03721184579387	23.21953159755541
11	35.445241850537386	21.83463222271867	21.54030697301946	21.17981895372448
12	21.406628214289082	24.812923439913064	30.38217094594154	23.39827739985632
13	30.754499636201427	19.83058657959021	24.439603999633665	24.975309784574698
14	22.210223138652566	20.646384644437944	25.869449594867817	31.273942622041673
15	25.061311719195345	27.440585808738366	22.847589541449477	24.65051293061681
16	24.232803056536305	26.19427061346033	24.95525313783822	24.61767319216515
17	23.910036031886687	26.244484724205403	25.703003591589646	24.14247565231827
18	23.965711350056388	25.363297158069813	26.477649283675653	24.193342208198143
19	24.93758878992934	25.179766758113697	26.76044800266004	23.122196449296915
20	24.3875533766573	25.061070072849123	26.654534409110646	23.896842141382926
21	23.975183886828315	25.4460851962857	26.954079219888268	23.624651696997713
22	24.574805130345247	24.97692881509439	26.231725797124845	24.21654025743552
23	23.991736661544568	25.26833014400431	26.41716520321612	24.322767991235004
24	23.88587139726442	25.286453619971006	26.496811838931112	24.330863143833465
25	24.282485545319684	25.01537474877842	26.323599737958702	24.378539967943198
26	23.871420945760303	25.332173108676336	26.578439974685132	24.217965970878232
27	24.249307501983314	25.688311493739306	26.21282905285023	23.849551951427152
28	24.00048425927783	25.58942980886499	26.148937758908957	24.261148172948225
29	23.896068873075016	25.828828844067786	26.56316792560386	23.71193435725334
30	24.206028641374832	25.584766034382895	26.23694535820325	23.972259966039022
31	24.086220382917634	25.02252748062661	26.611110560694435	24.280141575761323
32	23.561437012825863	25.62971225478031	26.518028388129462	24.290822344264367
33	23.65635569762213	25.03074345639818	26.635420183124438	24.67748066285526
34	24.03564380265323	24.985241449404448	27.003737544037026	23.975377203905296
35	23.847546286753506	24.97895864440266	26.8505820898011	24.322912979042737
36	23.536837414780397	24.637681509632387	27.22796118869704	24.59751988689018
37	23.34799079520738	24.300729844459497	27.59668934839821	24.754590011934912
38	23.295867678327152	24.174300476115796	27.942871903996807	24.586959941560245
39	22.902274109599666	24.149241750012504	28.527438580144064	24.421045560243762
40	23.198846670318748	23.958751935285175	28.559819190537905	24.282582203858176
41	22.54359843790136	24.11804520671516	28.790228981661215	24.548127373722263
42	22.156070192464064	24.399853175680036	28.692023906556326	24.752052725299574
43	21.92834267578382	24.102990639345485	28.592465611912587	25.376201072958104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2919.0
1	2523.0
2	2127.0
3	3858.0
4	5589.0
5	5589.0
6	6410.5
7	7232.0
8	6629.5
9	6027.0
10	8394.0
11	10761.0
12	10761.0
13	16774.0
14	22787.0
15	27924.5
16	33062.0
17	30832.0
18	28602.0
19	28602.0
20	32413.0
21	36224.0
22	32398.5
23	28573.0
24	33398.5
25	38224.0
26	38224.0
27	46010.0
28	53796.0
29	61758.0
30	69720.0
31	80651.5
32	91583.0
33	91583.0
34	105626.5
35	119670.0
36	135971.0
37	152272.0
38	170847.0
39	189422.0
40	189422.0
41	210034.0
42	230646.0
43	252168.5
44	273691.0
45	294245.0
46	314799.0
47	314799.0
48	334688.0
49	354577.0
50	362322.5
51	370068.0
52	364859.0
53	359650.0
54	359650.0
55	341724.5
56	323799.0
57	302087.0
58	280375.0
59	255378.0
60	230381.0
61	230381.0
62	201148.5
63	171916.0
64	146030.5
65	120145.0
66	99361.5
67	78578.0
68	78578.0
69	64430.0
70	50282.0
71	40999.5
72	31717.0
73	25848.5
74	19980.0
75	19980.0
76	16225.0
77	12470.0
78	10045.0
79	7620.0
80	5994.0
81	4368.0
82	4368.0
83	3434.0
84	2500.0
85	1931.0
86	1362.0
87	963.5
88	565.0
89	565.0
90	352.0
91	139.0
92	87.0
93	35.0
94	27.0
95	19.0
96	19.0
97	13.0
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4138279.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.11367552548072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.95174726473661	48.44468996553842
2	9.334496302926372	11.03592771289978
3	3.1786308874829436	5.637016646938126
4	1.5035559272683272	3.5552286887581275
5	0.89835286197203	2.6552469795000775
6	0.6052296273869041	2.146640868705423
7	0.43510664297693546	1.8004527038343827
8	0.32102518930477414	1.5181583100854754
9	0.2527713516169758	1.3448019295460791
>10	1.4223764864011144	15.19205878125794
>50	0.07314972716408488	2.905747803856259
>100	0.02277978947727022	2.180214340037963
>500	3.684985459575771E-4	0.14611755239633364
>1k	2.456656438454275E-4	0.3288551999337459
>5k	4.094427397423792E-5	0.17678357108383602
>10k+	1.2283282192271375E-4	0.9320589456280723
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14440	0.3489373239455339	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13613	0.32895317111291916	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10456	0.2526654196104226	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7304	0.17649849128103737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.832926924453378E-5
2	0.0	0.0	0.0	0.0	4.832926924453378E-5
3	0.0	0.0	0.0	0.0	4.832926924453378E-5
4	0.0	0.0	0.0	0.0	4.832926924453378E-5
5	0.0	2.416463462226689E-5	0.0	0.0	4.832926924453378E-5
6	0.0	2.416463462226689E-5	0.0	0.0	9.665853848906755E-5
7	0.0	2.416463462226689E-5	0.0	0.0	9.665853848906755E-5
8	0.0	2.416463462226689E-5	0.0	0.0	1.2082317311133445E-4
9	0.0	2.416463462226689E-5	0.0	0.0	1.691524423558682E-4
10	2.416463462226689E-5	2.416463462226689E-5	0.0	2.416463462226689E-5	1.691524423558682E-4
11	2.416463462226689E-5	2.416463462226689E-5	0.0	7.249390386680067E-5	1.691524423558682E-4
12	2.416463462226689E-5	2.416463462226689E-5	0.0	7.249390386680067E-5	1.691524423558682E-4
13	2.416463462226689E-5	2.416463462226689E-5	0.0	9.665853848906755E-5	1.691524423558682E-4
14	4.832926924453378E-5	2.416463462226689E-5	0.0	9.665853848906755E-5	1.933170769781351E-4
15	4.832926924453378E-5	2.416463462226689E-5	0.0	1.691524423558682E-4	2.416463462226689E-4
16	1.4498780773360134E-4	2.416463462226689E-5	0.0	2.17481711600402E-4	2.6581098084493577E-4
17	1.4498780773360134E-4	4.832926924453378E-5	0.0	3.866341539562702E-4	2.6581098084493577E-4
18	1.4498780773360134E-4	4.832926924453378E-5	0.0	5.316219616898715E-4	3.6246951933400335E-4
19	1.4498780773360134E-4	4.832926924453378E-5	0.0	6.282805001789391E-4	3.6246951933400335E-4
20	1.4498780773360134E-4	4.832926924453378E-5	0.0	8.940914810238749E-4	3.6246951933400335E-4
21	1.4498780773360134E-4	4.832926924453378E-5	0.0	0.0013532195388469457	3.6246951933400335E-4
22	1.691524423558682E-4	4.832926924453378E-5	0.0	0.0018365122312922835	3.6246951933400335E-4
23	1.933170769781351E-4	4.832926924453378E-5	0.0	0.002609780539204824	5.316219616898715E-4
24	1.933170769781351E-4	4.832926924453378E-5	0.0	0.0041804817896521716	5.316219616898715E-4
25	2.17481711600402E-4	4.832926924453378E-5	0.0	0.006282805001789391	5.316219616898715E-4
26	2.17481711600402E-4	4.832926924453378E-5	0.0	0.010269969714463428	5.316219616898715E-4
27	2.17481711600402E-4	4.832926924453378E-5	0.0	0.03689939706820154	5.316219616898715E-4
28	2.17481711600402E-4	4.832926924453378E-5	0.0	0.10881334970406781	5.316219616898715E-4
29	2.17481711600402E-4	4.832926924453378E-5	0.0	0.2083716443478074	5.316219616898715E-4
30	2.17481711600402E-4	4.832926924453378E-5	0.0	0.33252953703701466	5.316219616898715E-4
31	2.17481711600402E-4	4.832926924453378E-5	0.0	0.6924376051010577	5.557865963121384E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6040	0.0	17.581127	1
CTTATAC	3550	0.0	15.58169	37
TCTTATA	5550	0.0	13.766666	37
TTAGTAC	220	2.8558134E-10	13.454545	3
CTCTTAT	8380	0.0	12.428997	37
GTATTAC	345	0.0	12.333333	1
GTATCAA	8615	0.0	12.283227	2
GGACCGT	220	5.5351848E-8	11.772727	6
GTACCGA	190	1.4362686E-6	11.684211	6
ACCGTGC	400	0.0	11.562499	8
CATTACG	515	0.0	11.495146	24
ACGGATG	870	0.0	11.482759	28
GTAAGAG	1725	0.0	11.260869	3
GGGTAAG	1715	0.0	11.110787	1
TACGGAT	880	0.0	10.931818	27
TAAGAGT	615	0.0	10.829268	4
ATTACGG	550	0.0	10.763637	25
TAAGGCG	275	9.57516E-9	10.763637	5
TATACCG	225	9.377454E-7	10.688889	5
TATACAC	1580	0.0	10.655063	37
>>END_MODULE