FastQCFastQC Report
Thu 9 Feb 2017
SRR2727149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5925203
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT193830.327128032575424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT190560.3216092343165289No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT140600.23729144807359345No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99710.16828115424906118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102500.016.460491
CTTATAC53250.015.07793437
TCTTATA84450.013.88869237
GTATCAA137150.012.301862
CTCTTAT126150.011.93737537
AACGCAG147200.011.17289457
CTTACAC10450.010.9760763
TACTTAC10650.010.94366231
TATACCG2259.380037E-710.6888885
TATACAC23950.010.65970837
GTACCGT3851.8189894E-1210.5714286
TATTCCG2301.2443561E-610.4565225
TTATACT10900.010.353214
TCTACAC10900.010.353213
TCTCTTA171950.010.19947637
TATACTC5450.010.1834865
TAGTACT6000.010.1754
ACTATAC6550.010.1679393
CTAGTAC4550.010.1648363
TAGGACT9850.010.1421334
Produced by FastQC (version 0.11.5)