##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727149.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5925203
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.988739288763608	33.0	31.0	34.0	30.0	34.0
2	32.213226618564796	33.0	31.0	34.0	30.0	34.0
3	32.3216173353048	34.0	31.0	34.0	30.0	34.0
4	35.94040187315101	37.0	35.0	37.0	35.0	37.0
5	35.644090506266195	37.0	35.0	37.0	33.0	37.0
6	35.655182109372454	37.0	35.0	37.0	33.0	37.0
7	35.620163224787404	37.0	35.0	37.0	33.0	37.0
8	35.58787032275519	37.0	35.0	37.0	33.0	37.0
9	37.410697321256336	39.0	37.0	39.0	34.0	39.0
10	37.22914438543287	39.0	37.0	39.0	34.0	39.0
11	37.318932026463905	39.0	37.0	39.0	34.0	39.0
12	37.22127343147568	39.0	37.0	39.0	34.0	39.0
13	37.29864191994772	39.0	37.0	39.0	34.0	39.0
14	38.48353600712077	40.0	38.0	41.0	34.0	41.0
15	38.43572161156335	40.0	38.0	41.0	34.0	41.0
16	38.394691287370236	40.0	38.0	41.0	34.0	41.0
17	38.34911799646358	40.0	38.0	41.0	34.0	41.0
18	38.3508355747474	40.0	38.0	41.0	34.0	41.0
19	38.40029109551183	40.0	38.0	41.0	34.0	41.0
20	38.31519561439498	40.0	38.0	41.0	34.0	41.0
21	38.25584338629411	40.0	38.0	41.0	34.0	41.0
22	38.19608138320324	40.0	38.0	41.0	34.0	41.0
23	38.136774048079026	40.0	38.0	41.0	33.0	41.0
24	38.057357528509996	40.0	37.0	41.0	33.0	41.0
25	38.0244055773279	40.0	37.0	41.0	33.0	41.0
26	37.86533355903587	40.0	37.0	41.0	33.0	41.0
27	37.731347938627586	40.0	37.0	41.0	33.0	41.0
28	37.652845143027164	40.0	37.0	41.0	32.0	41.0
29	37.560879855086824	39.0	37.0	41.0	32.0	41.0
30	37.50837785642112	39.0	37.0	41.0	32.0	41.0
31	37.46303308089191	39.0	37.0	41.0	32.0	41.0
32	37.355978858445866	39.0	36.0	41.0	32.0	41.0
33	37.281856334711236	39.0	36.0	41.0	31.0	41.0
34	37.23934319212354	39.0	36.0	41.0	31.0	41.0
35	37.16950153437781	39.0	36.0	41.0	31.0	41.0
36	37.05764140064062	39.0	36.0	40.0	31.0	41.0
37	36.97673699955934	39.0	36.0	40.0	31.0	41.0
38	36.93663001925841	39.0	36.0	40.0	31.0	41.0
39	36.83817533340208	39.0	36.0	40.0	30.0	41.0
40	36.754018723071596	39.0	35.0	40.0	30.0	41.0
41	36.699019932312865	39.0	35.0	40.0	30.0	41.0
42	36.562341745928364	39.0	35.0	40.0	30.0	41.0
43	35.266328934215416	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	7.0
11	10.0
12	11.0
13	2.0
14	9.0
15	17.0
16	27.0
17	67.0
18	135.0
19	319.0
20	666.0
21	1552.0
22	2846.0
23	5288.0
24	8789.0
25	14483.0
26	22382.0
27	34285.0
28	49330.0
29	69772.0
30	95432.0
31	127521.0
32	167310.0
33	220756.0
34	296678.0
35	405833.0
36	577840.0
37	884502.0
38	1531600.0
39	1407725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.72113546151921	18.44866749713048	12.706349470220681	26.123847571129634
2	18.535044284558687	20.574349942103247	35.67317440431999	25.217431369018072
3	20.057253734597786	22.227829156233128	29.911278989091176	27.803638120077913
4	15.111330362858455	15.551990370625276	33.84955755946252	35.487121707053745
5	15.672374431728331	35.23430336479611	33.71980673067235	15.37351547280321
6	35.422870743837805	33.892003362585214	15.148223613604461	15.536902279972518
7	29.26750357751456	29.920831404426146	21.291878101054092	19.519786917005206
8	26.346439100905066	34.62053536393606	19.62714188864078	19.405883646518102
9	26.464207217879288	14.935893335637616	19.72612583906408	38.87377360741902
10	17.520564274337943	26.331148485545558	32.82101895918165	23.32726828093485
11	35.52004884895927	21.796704686742377	21.375183263763283	21.30806320053507
12	21.53637605327615	24.787252014825484	30.14079011301385	23.53558181888452
13	30.91554837868002	19.758732316850576	24.21884954827708	25.106869756192317
14	22.292923972393858	20.67672618136459	25.588524140016805	31.441825706224748
15	25.173989819420534	27.398436813050964	22.618803102611	24.808770264917506
16	24.441559217464786	26.067849489713684	24.696537823261078	24.794053469560453
17	23.985625471397352	26.231810116885445	25.574769337016807	24.207795074700396
18	24.124203001989976	25.313630604723585	26.258560255235135	24.303606138051304
19	24.992966485705214	25.18175326651256	26.521943636361488	23.30333661142074
20	24.508291108338398	25.13149338512115	26.392547225808126	23.967668280732322
21	24.186884398728616	25.40760206865486	26.568608704208106	23.83690482840841
22	24.70696784565862	25.091950436128517	25.966266472220447	24.234815245992415
23	24.18938220344518	25.261244213911322	26.088861428038832	24.46051215460466
24	24.122667189630466	25.3383048648291	26.131729157633927	24.407298787906505
25	24.520830763097905	25.04160616944263	25.9436512133002	24.493911854159258
26	24.09498881304151	25.423888430489217	26.224299825676862	24.256822930792413
27	24.38262452780099	25.59839721947079	25.959701296310016	24.059276956418202
28	24.20263069467831	25.552390356921105	25.96581079163026	24.279168156770325
29	24.021455467432933	25.734780732406975	26.304803396609366	23.93896040355073
30	24.24850254075683	25.640471727297783	26.070431679724727	24.04059405222066
31	24.22399705124027	25.05222183948803	26.309444587805686	24.414336521466016
32	23.703002243129898	25.621721314864654	26.23575934866704	24.439517093338406
33	23.880953952126198	25.123594921557963	26.26448410290753	24.73096702340831
34	24.131780801434143	24.977574607992334	26.6000506649308	24.290593925642717
35	23.90734967223908	24.976460722105216	26.62772904151976	24.48846056413595
36	23.700841979591246	24.585031095137165	26.985961493639966	24.728165431631624
37	23.509827427009675	24.192470705223094	27.387854897123354	24.909846970643876
38	23.324281041510307	24.117131514312675	27.813696847179752	24.74489059699727
39	22.958217634062493	24.037640566913907	28.397794978501157	24.606346820522436
40	23.109216680002355	23.959044103636618	28.421034688600542	24.51070452776048
41	22.543902715231866	24.06273675349182	28.627626766542853	24.76573376473346
42	22.202277289065034	24.37499609718013	28.522583951975992	24.900142661778844
43	22.019515618283457	24.099140569529855	28.510584363101145	25.37075944908554
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3773.0
1	3082.5
2	2392.0
3	4372.0
4	6352.0
5	6352.0
6	7636.5
7	8921.0
8	8342.0
9	7763.0
10	11072.0
11	14381.0
12	14381.0
13	22998.0
14	31615.0
15	39329.5
16	47044.0
17	43319.0
18	39594.0
19	39594.0
20	44456.0
21	49318.0
22	43887.0
23	38456.0
24	45495.0
25	52534.0
26	52534.0
27	62981.5
28	73429.0
29	84457.5
30	95486.0
31	110659.0
32	125832.0
33	125832.0
34	145388.0
35	164944.0
36	189055.5
37	213167.0
38	240347.5
39	267528.0
40	267528.0
41	298287.5
42	329047.0
43	360057.0
44	391067.0
45	422234.5
46	453402.0
47	453402.0
48	480842.0
49	508282.0
50	518526.0
51	528770.0
52	522531.0
53	516292.0
54	516292.0
55	491637.0
56	466982.0
57	437474.5
58	407967.0
59	372630.0
60	337293.0
61	337293.0
62	295761.0
63	254229.0
64	215839.5
65	177450.0
66	147825.0
67	118200.0
68	118200.0
69	96405.5
70	74611.0
71	60838.0
72	47065.0
73	38439.5
74	29814.0
75	29814.0
76	24032.0
77	18250.0
78	14730.0
79	11210.0
80	8696.5
81	6183.0
82	6183.0
83	4842.0
84	3501.0
85	2679.0
86	1857.0
87	1340.5
88	824.0
89	824.0
90	548.5
91	273.0
92	172.0
93	71.0
94	48.5
95	26.0
96	26.0
97	15.0
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5925203.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.84706811318362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87219487487084	43.80755712651517
2	10.380321314292177	11.386603803234294
3	3.4789811057350435	5.724357410121864
4	1.696562021461795	3.7220581099742223
5	1.0324662879604403	2.8313874410166058
6	0.6389164513071143	2.102561647408492
7	0.47970687820713737	1.841736107637268
8	0.35186578422500514	1.5439045307270103
9	0.2548482816635898	1.2579912956637642
>10	1.6667803593619812	17.065158610454542
>50	0.1130105595319667	4.224983339816738
>100	0.033638264125853204	2.976685921142369
>500	3.0774990301117E-4	0.11158786560929346
>1k	2.7696970667865327E-4	0.3490042425413614
>5k	3.077441184170405E-5	0.1682991392264074
>10k+	9.232323552511217E-5	0.8861234089105567
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19383	0.327128032575424	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19056	0.3216092343165289	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14060	0.23729144807359345	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9971	0.16828115424906118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6877058895703657E-5
2	0.0	0.0	0.0	0.0	3.3754117791407314E-5
3	0.0	0.0	0.0	0.0	3.3754117791407314E-5
4	0.0	0.0	0.0	0.0	3.3754117791407314E-5
5	0.0	1.6877058895703657E-5	0.0	0.0	5.063117668711097E-5
6	1.6877058895703657E-5	1.6877058895703657E-5	0.0	0.0	6.750823558281463E-5
7	1.6877058895703657E-5	1.6877058895703657E-5	0.0	0.0	8.438529447851828E-5
8	1.6877058895703657E-5	1.6877058895703657E-5	0.0	0.0	8.438529447851828E-5
9	3.3754117791407314E-5	1.6877058895703657E-5	0.0	1.6877058895703657E-5	1.3501647116562925E-4
10	3.3754117791407314E-5	1.6877058895703657E-5	0.0	1.6877058895703657E-5	1.3501647116562925E-4
11	3.3754117791407314E-5	1.6877058895703657E-5	0.0	1.6877058895703657E-5	1.6877058895703657E-4
12	3.3754117791407314E-5	1.6877058895703657E-5	0.0	5.063117668711097E-5	1.6877058895703657E-4
13	3.3754117791407314E-5	3.3754117791407314E-5	0.0	6.750823558281463E-5	1.6877058895703657E-4
14	3.3754117791407314E-5	3.3754117791407314E-5	0.0	8.438529447851828E-5	2.531558834355549E-4
15	3.3754117791407314E-5	3.3754117791407314E-5	0.0	1.518935300613329E-4	2.869100012269622E-4
16	5.063117668711097E-5	3.3754117791407314E-5	0.0	2.531558834355549E-4	3.206641190183695E-4
17	5.063117668711097E-5	3.3754117791407314E-5	0.0	4.2192647239259145E-4	3.206641190183695E-4
18	5.063117668711097E-5	3.3754117791407314E-5	0.0	4.894347079754061E-4	4.0504941349688776E-4
19	5.063117668711097E-5	3.3754117791407314E-5	0.0	7.088364736195536E-4	4.2192647239259145E-4
20	6.750823558281463E-5	3.3754117791407314E-5	0.0	9.282382392637011E-4	4.2192647239259145E-4
21	6.750823558281463E-5	3.3754117791407314E-5	0.0	0.0015020582417176256	4.2192647239259145E-4
22	6.750823558281463E-5	3.3754117791407314E-5	0.0	0.002362788245398512	4.388035312882951E-4
23	6.750823558281463E-5	3.3754117791407314E-5	0.0	0.0033247806024536207	6.075741202453316E-4
24	8.438529447851828E-5	3.3754117791407314E-5	0.0	0.0051137488453982085	6.41328238036739E-4
25	8.438529447851828E-5	3.3754117791407314E-5	0.0	0.007324643560735387	6.582052969324426E-4
26	8.438529447851828E-5	3.3754117791407314E-5	0.0	0.012117728287115227	6.582052969324426E-4
27	8.438529447851828E-5	3.3754117791407314E-5	0.0	0.04160195017790952	6.750823558281463E-4
28	8.438529447851828E-5	3.3754117791407314E-5	0.0	0.12006339698403583	6.750823558281463E-4
29	8.438529447851828E-5	3.3754117791407314E-5	0.0	0.22944361568709123	6.750823558281463E-4
30	8.438529447851828E-5	3.3754117791407314E-5	0.0	0.3651014150907572	6.750823558281463E-4
31	8.438529447851828E-5	3.3754117791407314E-5	0.0	0.7316711343054407	7.088364736195536E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10250	0.0	16.46049	1
CTTATAC	5325	0.0	15.077934	37
TCTTATA	8445	0.0	13.888692	37
GTATCAA	13715	0.0	12.30186	2
CTCTTAT	12615	0.0	11.937375	37
AACGCAG	14720	0.0	11.1728945	7
CTTACAC	1045	0.0	10.976076	3
TACTTAC	1065	0.0	10.943662	31
TATACCG	225	9.380037E-7	10.688888	5
TATACAC	2395	0.0	10.659708	37
GTACCGT	385	1.8189894E-12	10.571428	6
TATTCCG	230	1.2443561E-6	10.456522	5
TTATACT	1090	0.0	10.35321	4
TCTACAC	1090	0.0	10.35321	3
TCTCTTA	17195	0.0	10.199476	37
TATACTC	545	0.0	10.183486	5
TAGTACT	600	0.0	10.175	4
ACTATAC	655	0.0	10.167939	3
CTAGTAC	455	0.0	10.164836	3
TAGGACT	985	0.0	10.142133	4
>>END_MODULE