##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727148.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7881033
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89862382253697	31.0	31.0	34.0	30.0	34.0
2	32.12857451554891	33.0	31.0	34.0	30.0	34.0
3	32.23915684657075	34.0	31.0	34.0	30.0	34.0
4	35.87392274591414	37.0	35.0	37.0	35.0	37.0
5	35.564581571984284	37.0	35.0	37.0	33.0	37.0
6	35.581848851540144	37.0	35.0	37.0	33.0	37.0
7	35.539823776908435	37.0	35.0	37.0	33.0	37.0
8	35.50683457358953	37.0	35.0	37.0	33.0	37.0
9	37.32012313614218	39.0	37.0	39.0	34.0	39.0
10	37.13604371406642	39.0	37.0	39.0	33.0	39.0
11	37.225180379272615	39.0	37.0	39.0	34.0	39.0
12	37.123658662512895	39.0	37.0	39.0	33.0	39.0
13	37.20474232248488	39.0	37.0	39.0	34.0	39.0
14	38.36932366607271	40.0	38.0	41.0	34.0	41.0
15	38.320952342161235	40.0	38.0	41.0	34.0	41.0
16	38.278674001238166	40.0	38.0	41.0	33.0	41.0
17	38.23412463822953	40.0	38.0	41.0	33.0	41.0
18	38.237900919841344	40.0	38.0	41.0	33.0	41.0
19	38.27779873526732	40.0	38.0	41.0	34.0	41.0
20	38.191956181378764	40.0	38.0	41.0	34.0	41.0
21	38.14263688529156	40.0	38.0	41.0	33.0	41.0
22	38.073670291699074	40.0	37.0	41.0	33.0	41.0
23	38.01600538914125	40.0	37.0	41.0	33.0	41.0
24	37.927899426382304	40.0	37.0	41.0	33.0	41.0
25	37.896706053635356	40.0	37.0	41.0	33.0	41.0
26	37.73379111596157	40.0	37.0	41.0	33.0	41.0
27	37.58946612709273	39.0	37.0	41.0	32.0	41.0
28	37.5039981433906	39.0	37.0	41.0	32.0	41.0
29	37.404764705337485	39.0	37.0	41.0	32.0	41.0
30	37.35845034527834	39.0	36.0	41.0	32.0	41.0
31	37.29918222649239	39.0	36.0	41.0	31.0	41.0
32	37.18982739952999	39.0	36.0	41.0	31.0	41.0
33	37.11760590267799	39.0	36.0	40.0	31.0	41.0
34	37.07486455138559	39.0	36.0	40.0	31.0	41.0
35	36.99532129861657	39.0	36.0	40.0	31.0	41.0
36	36.89005146914116	39.0	36.0	40.0	30.0	41.0
37	36.803013767357655	39.0	35.0	40.0	30.0	41.0
38	36.7552809384252	39.0	35.0	40.0	30.0	41.0
39	36.653812133510925	39.0	35.0	40.0	30.0	41.0
40	36.56474830139653	39.0	35.0	40.0	30.0	41.0
41	36.50794864074291	39.0	35.0	40.0	30.0	41.0
42	36.38188420223592	39.0	35.0	40.0	30.0	41.0
43	35.07994523053006	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	7.0
10	15.0
11	15.0
12	17.0
13	19.0
14	13.0
15	25.0
16	49.0
17	91.0
18	201.0
19	519.0
20	1143.0
21	2236.0
22	4348.0
23	8068.0
24	13699.0
25	22071.0
26	34166.0
27	50773.0
28	73315.0
29	102740.0
30	138917.0
31	183714.0
32	239987.0
33	311429.0
34	411722.0
35	557497.0
36	783725.0
37	1178788.0
38	1994629.0
39	1767094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.63859826497364	18.5867766319466	12.808841176023497	25.965783927056265
2	18.438940682014653	20.731901515956093	35.805331610716514	25.023826191312736
3	20.022857409682207	22.41500574861189	29.87537293651733	27.68676390518857
4	15.075384153321018	15.675064423661214	33.974112276905835	35.275439146111935
5	15.587334807505565	35.359438286833715	33.717420038718274	15.335806866942441
6	35.15016369047053	34.09851221280256	15.20922447602998	15.54209962069693
7	29.150810559986233	30.028944175211546	21.40369162265911	19.416553642143107
8	26.213441816574047	34.75760093886169	19.646764580226982	19.38219266433728
9	26.3015140274124	15.057708805431979	19.894016431602303	38.74676073555332
10	17.544146814256457	26.3580548387502	32.84903133891204	23.248767008081302
11	35.282976229131386	21.87741124799249	21.567312305379257	21.27230021749687
12	21.453076011736023	24.993068802021256	30.229501640203765	23.32435354603895
13	30.81819350331359	19.799168459261622	24.431771824835653	24.950866212589137
14	22.230816696237664	20.729871325243785	25.815308729198318	31.224003249320237
15	25.0449528634127	27.482615540373956	22.761736944890345	24.710694651322992
16	24.32082190240797	26.10134991187069	24.814678481869066	24.763149703852275
17	23.84774940036414	26.351532851086905	25.777509115873514	24.023208632675438
18	24.05358536120836	25.267093793415153	26.447548183087164	24.231772662289323
19	24.843837095974603	25.174301389170683	26.801905283228734	23.17995623162598
20	24.431708381375895	25.13773257896522	26.652381737267184	23.778177302391704
21	24.12442632837599	25.403712939661588	26.747762634669847	23.724098097292575
22	24.51790266580536	25.198752498562055	26.240240333976523	24.043104501656064
23	23.997666295776202	25.327098617655828	26.345734118864872	24.32950096770309
24	24.03703930690304	25.398383689041783	26.33166235949018	24.232914644564996
25	24.358507317505204	25.111936468226943	26.26895484386374	24.260601370404107
26	23.980232033034248	25.485098209841272	26.459856214280542	24.07481354284394
27	24.22848629107377	25.631398828047036	26.231688155600924	23.90842672527827
28	24.09644776262198	25.54743775340111	26.301234876189454	24.054879607787456
29	23.95160634399069	25.798394702826393	26.489750772519287	23.760248180663627
30	24.02215547124343	25.709941831229482	26.382467882065715	23.885434815461373
31	24.079965151776424	25.141412299631277	26.54989517237144	24.228727376220856
32	23.56578128780834	25.66194050957533	26.51194329474321	24.26033490787312
33	23.756187799239008	25.196849194769268	26.543512760319615	24.50345024567211
34	23.993237942284978	25.094654469788413	26.828691111939264	24.08341647598735
35	23.76027355804753	25.139547061914346	26.849817784039224	24.2503615959989
36	23.578102007693662	24.738749349228712	27.13068198039521	24.552466662682416
37	23.435582619689576	24.33085865774195	27.479037329243518	24.75452139332496
38	23.121562871263198	24.365257701623634	27.93215051884696	24.581028908266216
39	22.968232717716067	24.247443196849954	28.382510262296833	24.401813823137143
40	22.982139524095384	24.17634845584329	28.527440501771785	24.31407151828954
41	22.49519828174809	24.2342596459119	28.726995560099798	24.543546512240212
42	22.150776427404885	24.52549050359261	28.57964178046203	24.744091288540474
43	21.998575567441474	24.257809858174685	28.57314517018264	25.1704694042012
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6204.0
1	5013.0
2	3822.0
3	6731.5
4	9641.0
5	9641.0
6	11588.0
7	13535.0
8	12396.5
9	11258.0
10	16116.5
11	20975.0
12	20975.0
13	33429.0
14	45883.0
15	56611.0
16	67339.0
17	61627.5
18	55916.0
19	55916.0
20	62826.5
21	69737.0
22	61155.0
23	52573.0
24	62240.0
25	71907.0
26	71907.0
27	86229.5
28	100552.0
29	115617.5
30	130683.0
31	151463.5
32	172244.0
33	172244.0
34	198746.0
35	225248.0
36	258339.5
37	291431.0
38	328876.0
39	366321.0
40	366321.0
41	406515.5
42	446710.0
43	487937.0
44	529164.0
45	565762.0
46	602360.0
47	602360.0
48	635918.5
49	669477.0
50	681222.5
51	692968.0
52	681946.5
53	670925.0
54	670925.0
55	640161.5
56	609398.0
57	570065.0
58	530732.0
59	484225.0
60	437718.0
61	437718.0
62	384325.0
63	330932.0
64	281938.0
65	232944.0
66	194313.0
67	155682.0
68	155682.0
69	127007.0
70	98332.0
71	80505.0
72	62678.0
73	51137.5
74	39597.0
75	39597.0
76	32039.5
77	24482.0
78	19649.5
79	14817.0
80	11562.0
81	8307.0
82	8307.0
83	6433.5
84	4560.0
85	3487.5
86	2415.0
87	1736.0
88	1057.0
89	1057.0
90	700.0
91	343.0
92	223.5
93	104.0
94	69.0
95	34.0
96	34.0
97	26.0
98	18.0
99	14.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7881033.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.746031671192114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87699717663229	40.02694596853913
2	10.428702048833237	10.584304889190351
3	3.765528269018551	5.732568503951538
4	1.7999337836146818	3.6535798715743724
5	1.0989749465343386	2.7884308721339104
6	0.7356357915513246	2.2398358305915593
7	0.46859900799629034	1.6645678070608265
8	0.37623992969513254	1.527414671062103
9	0.2943535911843235	1.3443548994691934
>10	1.9446023401146473	18.74406874362549
>50	0.15528999495924453	5.392581991883724
>100	0.053793830713498546	4.377709295560157
>500	9.496058850013174E-4	0.2903633013844783
>1k	2.4980205104360243E-4	0.3226920187956193
>5k	4.996040549379927E-5	0.13576490370890038
>10k+	9.992081098759853E-5	1.1748164314686984
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28390	0.36023196451531164	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28103	0.35659030992510754	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20862	0.26471149150117756	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15322	0.19441613808748168	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.8066075855792E-5
2	0.0	0.0	0.0	0.0	3.8066075855792E-5
3	0.0	0.0	0.0	0.0	3.8066075855792E-5
4	0.0	0.0	0.0	1.2688691951930668E-5	5.075476780772267E-5
5	0.0	1.2688691951930668E-5	0.0	1.2688691951930668E-5	5.075476780772267E-5
6	2.5377383903861335E-5	1.2688691951930668E-5	0.0	1.2688691951930668E-5	1.14198227567376E-4
7	2.5377383903861335E-5	1.2688691951930668E-5	0.0	1.2688691951930668E-5	1.14198227567376E-4
8	2.5377383903861335E-5	1.2688691951930668E-5	0.0	1.2688691951930668E-5	1.14198227567376E-4
9	2.5377383903861335E-5	1.2688691951930668E-5	0.0	2.5377383903861335E-5	1.2688691951930666E-4
10	2.5377383903861335E-5	1.2688691951930668E-5	0.0	3.8066075855792E-5	1.2688691951930666E-4
11	2.5377383903861335E-5	1.2688691951930668E-5	0.0	6.344345975965333E-5	1.3957561147123732E-4
12	2.5377383903861335E-5	1.2688691951930668E-5	0.0	7.6132151711584E-5	1.52264303423168E-4
13	2.5377383903861335E-5	1.2688691951930668E-5	0.0	8.882084366351467E-5	1.52264303423168E-4
14	2.5377383903861335E-5	1.2688691951930668E-5	0.0	1.0150953561544534E-4	2.537738390386133E-4
15	2.5377383903861335E-5	1.2688691951930668E-5	0.0	1.52264303423168E-4	2.66462530990544E-4
16	3.8066075855792E-5	1.2688691951930668E-5	0.0	2.7915122294247465E-4	3.2990599075019734E-4
17	3.8066075855792E-5	1.2688691951930668E-5	0.0	3.679720666059893E-4	3.2990599075019734E-4
18	3.8066075855792E-5	1.2688691951930668E-5	0.0	4.314155263656427E-4	3.5528337465405867E-4
19	3.8066075855792E-5	1.2688691951930668E-5	0.0	6.978780573561867E-4	3.5528337465405867E-4
20	3.8066075855792E-5	1.2688691951930668E-5	0.0	9.643405883467307E-4	3.5528337465405867E-4
21	3.8066075855792E-5	1.2688691951930668E-5	0.0	0.0017256621054625707	3.933494505098507E-4
22	3.8066075855792E-5	1.2688691951930668E-5	0.0	0.0027661348455208855	3.933494505098507E-4
23	3.8066075855792E-5	1.2688691951930668E-5	0.0	0.003502078978732864	5.202363700291574E-4
24	5.075476780772267E-5	1.2688691951930668E-5	0.0	0.005354628003714741	5.202363700291574E-4
25	5.075476780772267E-5	2.5377383903861335E-5	0.0	0.0071437335689369654	5.202363700291574E-4
26	5.075476780772267E-5	2.5377383903861335E-5	0.0	0.011597464444064629	5.32925061981088E-4
27	5.075476780772267E-5	2.5377383903861335E-5	0.0	0.037368197798435816	5.583024458849493E-4
28	5.075476780772267E-5	2.5377383903861335E-5	0.0	0.108526382264863	5.583024458849493E-4
29	6.344345975965333E-5	2.5377383903861335E-5	0.0	0.21032775779520274	5.7099113783688E-4
30	6.344345975965333E-5	2.5377383903861335E-5	0.0	0.3423662862469933	5.7099113783688E-4
31	6.344345975965333E-5	2.5377383903861335E-5	0.0	0.6942998462257423	5.963685217407413E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	6745	0.0	16.484062	37
GGTATCA	14025	0.0	16.026737	1
TCTTATA	10545	0.0	13.526316	37
TATACTG	1255	0.0	12.235061	5
TAGATCG	230	7.930794E-9	12.065218	5
GTATCAA	18715	0.0	11.980765	2
TATACAC	2835	0.0	11.811287	37
CTCTTAT	15745	0.0	11.808511	37
GTCTTAG	1160	0.0	11.801724	1
TACACCG	580	0.0	11.163793	5
TAGTACT	915	0.0	10.918033	4
TCGTTAC	460	0.0	10.858696	23
GTATTAG	880	0.0	10.721591	1
GGACCGT	440	0.0	10.511364	6
AACGCAG	20955	0.0	10.488189	7
TCTCTTA	22050	0.0	10.453969	37
CTATACT	1080	0.0	10.449075	4
GTACTAG	640	0.0	10.40625	1
GTCTTAC	805	0.0	10.111801	1
TCTATAC	970	0.0	10.108248	3
>>END_MODULE