FastQCFastQC Report
Thu 9 Feb 2017
SRR2727147.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727147.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5313198
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT137910.25956119083083296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125250.23573373324314284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91050.17136571985459603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72050.13560571241651448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48150.021.0934561
CTTATAC34800.015.89511437
TCTTATA57650.014.63313137
GTATCAA70700.014.2609622
CTCTTAT85750.012.18950437
GTATTAG5650.012.1150451
CTATACT7500.011.5933344
TATACTG8000.011.331255
CTAGTAC4000.011.13
TAAGACT8100.010.7345684
TCTATAC6050.010.7024793
AACGCAG96900.010.5577927
TATCAAC97800.010.5552153
TCAACGC98150.010.4610295
TCTCTTA120350.010.45284637
CAACGCA99350.010.3719176
ATCAACG99300.010.358514
TAGACAG10700.010.2009345
GTCTTAG6650.010.0150381
GTATAGG7600.09.9802621
Produced by FastQC (version 0.11.5)