##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727147.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5313198
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.941074659743528	31.0	31.0	33.0	28.0	34.0
2	31.184331357498817	31.0	31.0	34.0	28.0	34.0
3	31.26993215761957	31.0	31.0	34.0	28.0	34.0
4	35.10484438938658	37.0	35.0	37.0	32.0	37.0
5	34.298217194239704	35.0	35.0	37.0	30.0	37.0
6	34.314772383788444	35.0	35.0	37.0	30.0	37.0
7	34.29360340043793	35.0	35.0	37.0	30.0	37.0
8	34.2208265154056	35.0	35.0	37.0	30.0	37.0
9	35.884960244282254	37.0	35.0	39.0	31.0	39.0
10	35.53220734480439	37.0	35.0	39.0	30.0	39.0
11	35.702799331024366	37.0	35.0	39.0	30.0	39.0
12	35.53255139371806	37.0	35.0	39.0	30.0	39.0
13	35.69170431819029	37.0	35.0	39.0	30.0	39.0
14	36.57620909290412	38.0	36.0	40.0	31.0	41.0
15	36.49017710237789	38.0	36.0	40.0	31.0	41.0
16	36.422003659566236	38.0	35.0	40.0	30.0	41.0
17	36.33832543037169	38.0	35.0	40.0	30.0	41.0
18	36.31926835777624	38.0	35.0	40.0	30.0	41.0
19	36.36388818937295	38.0	35.0	40.0	30.0	41.0
20	36.24389887220465	38.0	35.0	40.0	30.0	41.0
21	36.152738331980096	38.0	34.0	40.0	29.0	41.0
22	36.064033186792585	38.0	34.0	40.0	29.0	41.0
23	35.974558072181765	38.0	34.0	40.0	29.0	41.0
24	35.844176708641385	38.0	34.0	40.0	29.0	41.0
25	35.7990989607389	38.0	34.0	40.0	29.0	41.0
26	35.53957315349437	38.0	34.0	40.0	27.0	41.0
27	35.35502046037057	38.0	34.0	40.0	27.0	41.0
28	35.22903513100773	38.0	34.0	40.0	27.0	41.0
29	35.09488334520942	38.0	33.0	40.0	27.0	40.0
30	35.01734529750256	38.0	33.0	40.0	26.0	40.0
31	34.96769102149026	38.0	33.0	40.0	26.0	40.0
32	34.83166108245919	37.0	33.0	40.0	25.0	40.0
33	34.73164598797184	37.0	33.0	40.0	25.0	40.0
34	34.68014837768139	37.0	33.0	40.0	25.0	40.0
35	34.57760956772174	37.0	33.0	39.0	25.0	40.0
36	34.423385501537865	37.0	33.0	39.0	25.0	40.0
37	34.32392374611298	37.0	33.0	39.0	24.0	40.0
38	34.265381037936095	37.0	33.0	39.0	24.0	40.0
39	34.142545035965156	37.0	32.0	39.0	24.0	40.0
40	34.04081120259399	37.0	32.0	39.0	24.0	40.0
41	33.9864211723335	37.0	32.0	39.0	24.0	40.0
42	33.821931349066986	37.0	32.0	39.0	24.0	40.0
43	32.50358089421851	35.0	30.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	5.0
11	8.0
12	7.0
13	17.0
14	37.0
15	65.0
16	191.0
17	386.0
18	850.0
19	1734.0
20	3473.0
21	6458.0
22	11408.0
23	18938.0
24	29690.0
25	44030.0
26	63490.0
27	88013.0
28	117948.0
29	154788.0
30	197754.0
31	247938.0
32	308998.0
33	386287.0
34	485916.0
35	617257.0
36	771629.0
37	874437.0
38	707221.0
39	174219.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.98983324167479	20.003150644865862	13.35180808243924	25.65520803102011
2	19.03955019180539	21.473282192758486	35.08045437041872	24.406713245017407
3	20.397677632190632	23.05908795418503	29.823375676946352	26.71985873667799
4	15.919489542832771	16.47700687984901	33.8721048980294	33.73139867928882
5	15.504202930137367	35.68854388637502	33.71067669603128	15.096576487456332
6	34.975564622285866	34.79757389052695	15.161772627332917	15.065088859854272
7	28.962274697837348	30.26448477922336	21.408142516051537	19.365098006887756
8	25.806962209953404	35.40184273200434	19.63435204183996	19.156843016202295
9	26.138363373621686	15.148259108732631	19.885688430960037	38.827689086685645
10	17.19021199661673	26.778128727745514	33.24476144122617	22.786897834411594
11	35.52536532611809	21.95180755544966	21.613517885085404	20.909309233346846
12	21.22525830959057	25.056566685450083	30.39066114231015	23.327513862649198
13	30.56592282086984	20.11856136360813	24.41802470000177	24.897491115520257
14	22.083611414443805	20.91672472962611	25.63761034314927	31.362053512780815
15	24.812570508383086	27.824955892853982	22.627878727651407	24.734594871111522
16	24.400935180657676	26.45122579659181	24.652685633021772	24.495153389728745
17	23.817538890890194	26.62931063363345	25.657617126258046	23.895533349218308
18	24.01356019482052	25.68867186955954	26.22781985538653	24.06994808023341
19	24.719857983835723	25.593700818226612	26.538028509383615	23.14841268855405
20	24.37646780714741	25.474619993457804	26.41636919986795	23.73254299952684
21	24.146963843621112	25.57839929925442	26.473001759015947	23.80163509810852
22	24.560970624471366	25.416594676125374	26.038141247512325	23.984293451890935
23	24.156092056046095	25.52826000461492	25.958942241565246	24.35670569777373
24	24.1198803432509	25.667931065245455	25.96803281187714	24.24415577962651
25	24.50563671822507	25.352621904924305	25.93692160540601	24.20481977144462
26	24.129667292655007	25.617038928344098	26.134072172729116	24.11922160627178
27	24.34322982128654	25.798624481903364	25.83886390079948	24.019281796010613
28	24.227047439225867	25.62985606785217	26.031120993420537	24.11197549950143
29	24.108851204114735	25.9175923803329	26.14263575345771	23.830920662094655
30	24.207153582456368	25.782137236368758	25.996621996771058	24.014087184403817
31	24.283868208939325	25.269244624423937	26.23276979325822	24.214117373378517
32	23.82981021975842	25.6666512334003	26.202693744897143	24.30084480194414
33	23.988829326518605	25.29469069287461	26.19473243797803	24.521747542628752
34	24.24306415834682	25.12861368990954	26.51344820953407	24.11487394220957
35	24.020204027781386	25.16234102324062	26.512281304028196	24.3051736449498
36	23.880645893490136	24.844415736059524	26.73920678280764	24.535731587642697
37	23.74110658025543	24.49234905230334	27.104015321845715	24.662529045595516
38	23.49249924433458	24.42323813266511	27.571153945326337	24.513108677673973
39	23.29892467775528	24.339484431033814	27.948497308024283	24.413093583186622
40	23.31721874471834	24.280047534460415	28.066862932644334	24.335870788176912
41	22.91890872502775	24.337639967492272	28.167028595584053	24.576422711895923
42	22.591704656969306	24.595883684364857	28.066900574757426	24.74551108390841
43	22.610337502950202	24.343248642343084	28.006823762261448	25.039590092445263
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4486.0
1	3509.5
2	2533.0
3	3672.5
4	4812.0
5	4812.0
6	5794.0
7	6776.0
8	6334.5
9	5893.0
10	8160.0
11	10427.0
12	10427.0
13	16611.0
14	22795.0
15	27666.0
16	32537.0
17	30993.0
18	29449.0
19	29449.0
20	34868.0
21	40287.0
22	38493.5
23	36700.0
24	43932.5
25	51165.0
26	51165.0
27	61756.0
28	72347.0
29	83622.0
30	94897.0
31	110051.0
32	125205.0
33	125205.0
34	144651.5
35	164098.0
36	186937.0
37	209776.0
38	234828.5
39	259881.0
40	259881.0
41	285655.0
42	311429.0
43	337793.5
44	364158.0
45	387739.5
46	411321.0
47	411321.0
48	431659.5
49	451998.0
50	458695.5
51	465393.0
52	458142.5
53	450892.0
54	450892.0
55	430471.0
56	410050.0
57	382680.5
58	355311.0
59	322355.0
60	289399.0
61	289399.0
62	253101.0
63	216803.0
64	184742.5
65	152682.0
66	126496.5
67	100311.0
68	100311.0
69	81642.5
70	62974.0
71	51106.0
72	39238.0
73	31730.5
74	24223.0
75	24223.0
76	19462.0
77	14701.0
78	11768.5
79	8836.0
80	6744.0
81	4652.0
82	4652.0
83	3589.5
84	2527.0
85	1927.0
86	1327.0
87	976.0
88	625.0
89	625.0
90	414.0
91	203.0
92	134.0
93	65.0
94	38.5
95	12.0
96	12.0
97	6.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5313198.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.74170193880121
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.91734206440525	50.365404772337264
2	8.852664562422074	10.754518244296593
3	2.9449348262366137	5.366410603333791
4	1.4495455294440884	3.5219144998485836
5	0.8854698831943946	2.689247386038951
6	0.5584789979933099	2.0353778901113992
7	0.40633261735413645	1.727693431193654
8	0.3025708830508555	1.4702936314907953
9	0.22400876942996759	1.2246006513953462
>10	1.3671372506037291	15.102077758496893
>50	0.07339679246955134	3.010226915017388
>100	0.017683890869005063	1.6043099689639868
>500	9.298600144747388E-5	0.04083589803947128
>1k	2.1696597080997034E-4	0.2845697234499339
>5k	6.199027737427594E-5	0.3070679582843769
>10k+	6.199027737427594E-5	0.4954506677015121
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13791	0.25956119083083296	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12525	0.23573373324314284	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9105	0.17136571985459603	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7205	0.13560571241651448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.882105654635871E-5	0.0	0.0	0.0	1.882105654635871E-5
3	1.882105654635871E-5	0.0	0.0	0.0	1.882105654635871E-5
4	1.882105654635871E-5	0.0	0.0	0.0	1.882105654635871E-5
5	1.882105654635871E-5	0.0	0.0	0.0	1.882105654635871E-5
6	3.764211309271742E-5	0.0	0.0	0.0	5.646316963907613E-5
7	3.764211309271742E-5	0.0	0.0	0.0	5.646316963907613E-5
8	5.646316963907613E-5	0.0	0.0	0.0	7.528422618543484E-5
9	5.646316963907613E-5	0.0	0.0	0.0	1.1292633927815225E-4
10	5.646316963907613E-5	0.0	0.0	0.0	1.1292633927815225E-4
11	5.646316963907613E-5	0.0	0.0	0.0	1.1292633927815225E-4
12	5.646316963907613E-5	0.0	0.0	1.882105654635871E-5	1.1292633927815225E-4
13	5.646316963907613E-5	0.0	0.0	1.882105654635871E-5	1.1292633927815225E-4
14	5.646316963907613E-5	0.0	0.0	1.882105654635871E-5	1.882105654635871E-4
15	5.646316963907613E-5	0.0	0.0	3.764211309271742E-5	2.070316220099458E-4
16	5.646316963907613E-5	0.0	0.0	7.528422618543484E-5	3.0113690474173934E-4
17	5.646316963907613E-5	0.0	0.0	1.3174739582451096E-4	3.0113690474173934E-4
18	5.646316963907613E-5	0.0	0.0	1.3174739582451096E-4	3.387790178344568E-4
19	5.646316963907613E-5	0.0	0.0	1.693895089172284E-4	3.576000743808155E-4
20	5.646316963907613E-5	0.0	0.0	3.0113690474173934E-4	3.576000743808155E-4
21	5.646316963907613E-5	0.0	0.0	5.646316963907613E-4	3.9524218747353287E-4
22	5.646316963907613E-5	0.0	0.0	9.410528273179355E-4	3.9524218747353287E-4
23	5.646316963907613E-5	0.0	0.0	0.0014680424106159793	4.7052641365896773E-4
24	7.528422618543484E-5	0.0	0.0	0.00233381101174848	4.893474702053264E-4
25	7.528422618543484E-5	0.0	0.0	0.0038206744789108177	5.081685267516852E-4
26	7.528422618543484E-5	0.0	0.0	0.006869685639420929	5.081685267516852E-4
27	7.528422618543484E-5	0.0	0.0	0.028099837423713553	5.081685267516852E-4
28	7.528422618543484E-5	0.0	0.0	0.08456300706278969	5.081685267516852E-4
29	7.528422618543484E-5	0.0	0.0	0.15994133853095632	5.269895832980438E-4
30	7.528422618543484E-5	0.0	0.0	0.2548182845811506	5.269895832980438E-4
31	7.528422618543484E-5	0.0	0.0	0.5369459222110676	5.458106398444026E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4815	0.0	21.093456	1
CTTATAC	3480	0.0	15.895114	37
TCTTATA	5765	0.0	14.633131	37
GTATCAA	7070	0.0	14.260962	2
CTCTTAT	8575	0.0	12.189504	37
GTATTAG	565	0.0	12.115045	1
CTATACT	750	0.0	11.593334	4
TATACTG	800	0.0	11.33125	5
CTAGTAC	400	0.0	11.1	3
TAAGACT	810	0.0	10.734568	4
TCTATAC	605	0.0	10.702479	3
AACGCAG	9690	0.0	10.557792	7
TATCAAC	9780	0.0	10.555215	3
TCAACGC	9815	0.0	10.461029	5
TCTCTTA	12035	0.0	10.452846	37
CAACGCA	9935	0.0	10.371917	6
ATCAACG	9930	0.0	10.35851	4
TAGACAG	1070	0.0	10.200934	5
GTCTTAG	665	0.0	10.015038	1
GTATAGG	760	0.0	9.980262	1
>>END_MODULE