FastQCFastQC Report
Thu 9 Feb 2017
SRR2727146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5811233
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT134570.2315687565788534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT124480.21420583204975605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95880.16499080315657624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75040.12912922266238505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44850.022.3979951
CTTATAC39950.015.88360637
GTATCAA66550.014.9278742
TCTTATA63000.013.36111137
CTCTTAT95100.012.60567937
TCGTTAC4900.012.08163423
TAGACAG12700.011.6535435
TATACAG10600.011.3443395
GTACCGT3700.011.0000016
TATCAAC93700.010.9578443
TCAACGC92800.010.8647635
ATCAACG92750.010.8307284
CAACGCA93950.010.8105376
CGCGAAA1200.00716826410.79166714
AACGCAG94000.010.7851077
CTATACT7450.010.6778524
TATACTG8900.010.6011235
TCTCTTA132150.010.499433537
GTACTAC6400.010.406251
TTATACT11250.010.3600014
Produced by FastQC (version 0.11.5)