##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727146.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5811233
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.459632577114014	31.0	31.0	34.0	30.0	34.0
2	31.69637459038383	31.0	31.0	34.0	30.0	34.0
3	31.80009285464892	31.0	31.0	34.0	30.0	34.0
4	35.525586738649096	37.0	35.0	37.0	33.0	37.0
5	35.055434190988386	37.0	35.0	37.0	32.0	37.0
6	35.06672129649594	37.0	35.0	37.0	32.0	37.0
7	35.02590362492779	37.0	35.0	37.0	32.0	37.0
8	34.97831905896735	36.0	35.0	37.0	32.0	37.0
9	36.729592842001	38.0	37.0	39.0	32.0	39.0
10	36.46968104703425	38.0	35.0	39.0	32.0	39.0
11	36.59030605036831	38.0	35.0	39.0	32.0	39.0
12	36.458948729125126	38.0	35.0	39.0	32.0	39.0
13	36.56631183089716	38.0	35.0	39.0	32.0	39.0
14	37.616281432873194	39.0	37.0	40.0	32.0	41.0
15	37.550301803421064	39.0	37.0	40.0	32.0	41.0
16	37.49551652807588	39.0	36.0	40.0	32.0	41.0
17	37.42682026344495	39.0	36.0	40.0	32.0	41.0
18	37.41188057680702	39.0	36.0	40.0	32.0	41.0
19	37.45373554975338	39.0	36.0	40.0	32.0	41.0
20	37.347087098383426	39.0	36.0	40.0	32.0	41.0
21	37.28887002121581	39.0	36.0	40.0	32.0	41.0
22	37.2137702962521	39.0	36.0	40.0	31.0	41.0
23	37.14823342997949	39.0	36.0	40.0	31.0	41.0
24	37.0300208234638	39.0	36.0	40.0	31.0	41.0
25	36.992316260593924	39.0	36.0	40.0	31.0	41.0
26	36.7895326172604	39.0	36.0	40.0	31.0	41.0
27	36.62192653435166	39.0	36.0	40.0	30.0	41.0
28	36.52304338855455	39.0	35.0	40.0	30.0	41.0
29	36.39789559289741	38.0	35.0	40.0	30.0	41.0
30	36.35398184860252	38.0	35.0	40.0	30.0	41.0
31	36.31218555511369	38.0	35.0	40.0	30.0	41.0
32	36.19400340685015	38.0	35.0	40.0	30.0	41.0
33	36.11838812864671	38.0	35.0	40.0	29.0	41.0
34	36.08163844058567	38.0	35.0	40.0	29.0	41.0
35	35.9937023691874	38.0	35.0	40.0	29.0	41.0
36	35.8706852745364	38.0	35.0	40.0	29.0	41.0
37	35.78676762745531	38.0	34.0	40.0	28.0	41.0
38	35.74624645062416	38.0	34.0	40.0	28.0	41.0
39	35.64638537122845	38.0	34.0	40.0	27.0	41.0
40	35.56201721734441	38.0	34.0	40.0	27.0	41.0
41	35.50873540950776	38.0	34.0	40.0	27.0	41.0
42	35.38786002901622	38.0	34.0	40.0	27.0	41.0
43	34.04065264634889	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	6.0
11	7.0
12	9.0
13	13.0
14	16.0
15	33.0
16	97.0
17	168.0
18	379.0
19	814.0
20	1764.0
21	3561.0
22	6515.0
23	11392.0
24	18428.0
25	28817.0
26	42385.0
27	60853.0
28	84306.0
29	113884.0
30	148147.0
31	189808.0
32	238863.0
33	301397.0
34	387104.0
35	508621.0
36	695287.0
37	998346.0
38	1368276.0
39	601934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.65546279077091	19.28807535337165	12.947802987765247	26.108658868092192
2	18.66440392254105	20.976994038958686	35.51038135968735	24.84822067881291
3	20.094393048772954	22.689401715608373	29.812399537241063	27.40380569837761
4	15.424970914090006	15.936549093798167	34.10734004298227	34.53113994912956
5	15.499481779512198	35.57637423933957	33.81103459455162	15.113109386596612
6	35.22904691654938	34.50212717335546	15.193557029979695	15.075268880115459
7	29.2364804508785	30.1480598007342	21.363658968759296	19.251800779628006
8	26.09305116487327	35.14047707259372	19.662694646729875	19.103777115803137
9	26.51633482945874	14.935539497383774	19.597218008639476	38.95090766451801
10	17.075360771113463	26.530290559679848	33.291213757906455	23.103134911300234
11	35.80436372108983	21.720192599401884	21.55478880299585	20.920654876512437
12	21.41667697715786	24.757190083412592	30.389505979195807	23.43662696023374
13	30.661857819158172	19.955231531759267	24.31757597742166	25.065334671660906
14	22.215939371214336	20.547102482381966	25.595463131490337	31.641495014913357
15	24.95306934001786	27.59333518377253	22.546420011037245	24.907175465172365
16	24.535928950018008	26.252156814225142	24.50891230828294	24.70300192747391
17	24.061055545355003	26.291546045391744	25.458710741765127	24.18868766748812
18	24.2060161759131	25.456938312402894	26.098798654261497	24.238246857422514
19	24.953275836642586	25.317449842400052	26.326289102502	23.40298521845536
20	24.542898211102532	25.175156459911346	26.21161464357048	24.07033068541564
21	24.303207253951097	25.341868068273982	26.411675456826462	23.943249220948463
22	24.725871428662387	25.07223510053718	25.8672471057347	24.334646365065726
23	24.253303902975496	25.258150894999392	25.928094777820814	24.560450424204294
24	24.239313756650265	25.396469217462116	25.924808039877252	24.43940898601037
25	24.63633793379133	25.079651770975282	25.861396368034118	24.422613927199272
26	24.27527858545682	25.372412360681462	25.965074881698946	24.387234172162774
27	24.505814858912043	25.63369942316889	25.67482666759361	24.185659050325466
28	24.324080621100546	25.475092807326778	25.783788053241025	24.41703851833165
29	24.29332983206834	25.694116893953485	25.984984597933003	24.02756867604517
30	24.396543728327533	25.555488826553674	25.83656514891074	24.21140229620805
31	24.41593720299978	25.033585815609182	26.076015193333323	24.474461788057717
32	23.917471558961758	25.503021475821054	26.048964135494135	24.530542829723057
33	24.078814255081497	25.020456071886983	26.140011938946518	24.760717734085002
34	24.353041772718456	24.929494308694903	26.46553665977599	24.251927258810653
35	24.165714917987284	24.90913718310727	26.431774461633182	24.49337343727226
36	24.003529027316578	24.60395237981337	26.66490915094955	24.7276094419205
37	23.876275482328793	24.249827876459264	26.96181688120232	24.91207976000962
38	23.65651144946348	24.17868978924094	27.40798725502832	24.75681150626726
39	23.44411246288008	24.114744667783928	27.805854626720354	24.635288242615637
40	23.508487785638607	24.031165158925823	27.957337109009394	24.50300994642617
41	23.029398408220768	24.10412729966257	28.13110401871685	24.73537027339981
42	22.719137229569007	24.355691812735785	28.024207599316703	24.900963358378505
43	22.603516327774155	24.078263597415557	27.99910105136036	25.319119023449925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3610.0
1	3023.0
2	2436.0
3	3875.0
4	5314.0
5	5314.0
6	6432.0
7	7550.0
8	6790.5
9	6031.0
10	8245.5
11	10460.0
12	10460.0
13	16594.5
14	22729.0
15	27174.5
16	31620.0
17	30442.5
18	29265.0
19	29265.0
20	34702.0
21	40139.0
22	38809.0
23	37479.0
24	45244.5
25	53010.0
26	53010.0
27	63607.5
28	74205.0
29	86199.0
30	98193.0
31	114359.0
32	130525.0
33	130525.0
34	150373.5
35	170222.0
36	194646.5
37	219071.0
38	247344.5
39	275618.0
40	275618.0
41	305146.5
42	334675.0
43	365163.0
44	395651.0
45	423395.0
46	451139.0
47	451139.0
48	475090.5
49	499042.0
50	509859.5
51	520677.0
52	513219.0
53	505761.0
54	505761.0
55	482745.5
56	459730.0
57	428520.0
58	397310.0
59	360717.5
60	324125.0
61	324125.0
62	283404.0
63	242683.0
64	206353.5
65	170024.0
66	141220.0
67	112416.0
68	112416.0
69	91362.0
70	70308.0
71	57436.5
72	44565.0
73	35972.5
74	27380.0
75	27380.0
76	22005.0
77	16630.0
78	13371.5
79	10113.0
80	7823.0
81	5533.0
82	5533.0
83	4311.5
84	3090.0
85	2404.5
86	1719.0
87	1258.0
88	797.0
89	797.0
90	528.5
91	260.0
92	174.0
93	88.0
94	58.0
95	28.0
96	28.0
97	18.5
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5811233.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.33800115917644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.72097425678615	47.67438290921419
2	9.34457341867421	10.902874698612507
3	3.2217404754650234	5.638496987767326
4	1.5783679852031134	3.683153334015449
5	0.949044237388521	2.7682671910440626
6	0.6010179187734509	2.103731042525484
7	0.4288984848418538	1.7514756214111162
8	0.3133430353815068	1.4623845089044967
9	0.23414959684453363	1.2293837506923389
>10	1.4945584947909099	15.930022894231014
>50	0.0884121064652692	3.474500673522512
>100	0.02429990488633931	2.273759454787652
>500	2.952818362784248E-4	0.10754255074764285
>1k	2.0669260367454218E-4	0.2593682973070976
>5k	5.9055029573847016E-5	0.2944227227139321
>10k+	5.9055029573847016E-5	0.4462333625031834
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13457	0.2315687565788534	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12448	0.21420583204975605	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9588	0.16499080315657624	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7504	0.12912922266238505	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.720805206055238E-5
3	0.0	0.0	0.0	0.0	1.720805206055238E-5
4	0.0	0.0	0.0	0.0	1.720805206055238E-5
5	0.0	0.0	0.0	0.0	1.720805206055238E-5
6	0.0	0.0	0.0	0.0	1.0324831236331429E-4
7	0.0	0.0	0.0	0.0	1.0324831236331429E-4
8	0.0	0.0	0.0	0.0	1.2045636442386668E-4
9	0.0	0.0	0.0	0.0	1.5487246854497145E-4
10	0.0	0.0	0.0	0.0	1.5487246854497145E-4
11	0.0	0.0	0.0	0.0	1.5487246854497145E-4
12	0.0	0.0	0.0	0.0	1.5487246854497145E-4
13	0.0	0.0	0.0	0.0	1.5487246854497145E-4
14	0.0	0.0	0.0	0.0	2.4091272884773336E-4
15	0.0	1.720805206055238E-5	0.0	3.441610412110476E-5	2.581207809082857E-4
16	1.720805206055238E-5	1.720805206055238E-5	0.0	1.2045636442386668E-4	2.753288329688381E-4
17	1.720805206055238E-5	1.720805206055238E-5	0.0	2.753288329688381E-4	2.753288329688381E-4
18	1.720805206055238E-5	1.720805206055238E-5	0.0	3.2695298915049527E-4	3.2695298915049527E-4
19	1.720805206055238E-5	1.720805206055238E-5	0.0	3.613690932716E-4	3.2695298915049527E-4
20	1.720805206055238E-5	3.441610412110476E-5	0.0	4.646174056349143E-4	3.2695298915049527E-4
21	1.720805206055238E-5	3.441610412110476E-5	0.0	6.022818221193334E-4	3.4416104121104765E-4
22	1.720805206055238E-5	3.441610412110476E-5	0.0	8.948187071487238E-4	3.4416104121104765E-4
23	1.720805206055238E-5	3.441610412110476E-5	0.0	0.0013766441648441906	4.3020130151380954E-4
24	1.720805206055238E-5	3.441610412110476E-5	0.0	0.0023919192364167813	4.3020130151380954E-4
25	1.720805206055238E-5	3.441610412110476E-5	0.0	0.003648107036837105	4.474093535743619E-4
26	1.720805206055238E-5	3.441610412110476E-5	0.0	0.006676724199494324	4.818254576954667E-4
27	1.720805206055238E-5	3.441610412110476E-5	0.0	0.029029983826151868	4.818254576954667E-4
28	1.720805206055238E-5	3.441610412110476E-5	0.0	0.08831172317475483	4.818254576954667E-4
29	1.720805206055238E-5	3.441610412110476E-5	0.0	0.1654726286142717	4.818254576954667E-4
30	1.720805206055238E-5	3.441610412110476E-5	0.0	0.26364456561972305	4.818254576954667E-4
31	1.720805206055238E-5	3.441610412110476E-5	0.0	0.5654565907097513	4.818254576954667E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4485	0.0	22.397995	1
CTTATAC	3995	0.0	15.883606	37
GTATCAA	6655	0.0	14.927874	2
TCTTATA	6300	0.0	13.361111	37
CTCTTAT	9510	0.0	12.605679	37
TCGTTAC	490	0.0	12.081634	23
TAGACAG	1270	0.0	11.653543	5
TATACAG	1060	0.0	11.344339	5
GTACCGT	370	0.0	11.000001	6
TATCAAC	9370	0.0	10.957844	3
TCAACGC	9280	0.0	10.864763	5
ATCAACG	9275	0.0	10.830728	4
CAACGCA	9395	0.0	10.810537	6
CGCGAAA	120	0.007168264	10.791667	14
AACGCAG	9400	0.0	10.785107	7
CTATACT	745	0.0	10.677852	4
TATACTG	890	0.0	10.601123	5
TCTCTTA	13215	0.0	10.4994335	37
GTACTAC	640	0.0	10.40625	1
TTATACT	1125	0.0	10.360001	4
>>END_MODULE