FastQCFastQC Report
Thu 9 Feb 2017
SRR2727145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188456
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95120.4346443337220397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT76490.3495158230277419No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60760.27763866397131126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46360.2118388489419024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.025.7594931
GTATCAA34950.017.5207442
CTTATAC16000.017.45937337
ATACCGT1302.5918962E-715.6538466
CTCTTAT39600.013.73484937
TACTTAC8800.013.45454531
GAGTGTC8300.013.3734947
TCTTATA25100.013.266932537
AGTGTCT10000.012.958
CGACCGA2152.903107E-912.90697718
TACGGAT8650.012.83237127
GTTACGG7500.012.82666825
CGTTACG4250.012.62352924
GGAGTGT8650.012.6184986
GGGTAAG12550.012.52988151
TCGTTAC5200.012.45192323
GTACTTA9150.012.33333430
TCGGACG2255.7134457E-912.33333313
AGTGTAC10850.012.27649927
TATCAAC50900.012.2121823
Produced by FastQC (version 0.11.5)