##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727144.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3980307
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44091900448885	31.0	31.0	34.0	30.0	34.0
2	31.69190592584944	31.0	31.0	34.0	30.0	34.0
3	31.80194643277516	31.0	31.0	34.0	30.0	34.0
4	35.55424016288191	37.0	35.0	37.0	33.0	37.0
5	35.12471701303442	37.0	35.0	37.0	32.0	37.0
6	35.138296116354844	37.0	35.0	37.0	32.0	37.0
7	35.08649860425339	36.0	35.0	37.0	32.0	37.0
8	35.034128774489005	36.0	35.0	37.0	32.0	37.0
9	36.848087346026325	39.0	37.0	39.0	33.0	39.0
10	36.590600926008975	38.0	35.0	39.0	32.0	39.0
11	36.70607970691708	39.0	35.0	39.0	32.0	39.0
12	36.579522132338035	38.0	35.0	39.0	32.0	39.0
13	36.72968391634112	39.0	35.0	39.0	32.0	39.0
14	37.73994870244933	39.0	37.0	40.0	33.0	41.0
15	37.73885732934671	39.0	37.0	40.0	33.0	41.0
16	37.667473890833044	39.0	37.0	40.0	32.0	41.0
17	37.603515256486496	39.0	37.0	40.0	32.0	41.0
18	37.602022155577444	39.0	37.0	40.0	32.0	41.0
19	37.63655843632162	39.0	37.0	40.0	32.0	41.0
20	37.512438362166534	39.0	37.0	40.0	32.0	41.0
21	37.522920719431944	39.0	37.0	40.0	32.0	41.0
22	37.43167373772928	39.0	36.0	40.0	32.0	41.0
23	37.36387343991305	39.0	36.0	40.0	32.0	41.0
24	37.24521173869252	39.0	36.0	40.0	31.0	41.0
25	37.2174279521655	39.0	36.0	40.0	31.0	41.0
26	37.07641521118848	39.0	36.0	40.0	31.0	41.0
27	36.87059616255731	39.0	36.0	40.0	31.0	41.0
28	36.756455971863474	39.0	36.0	40.0	30.0	41.0
29	36.645463779552685	39.0	35.0	40.0	30.0	41.0
30	36.62981473539604	39.0	35.0	40.0	30.0	41.0
31	36.568228531115814	38.0	35.0	40.0	30.0	41.0
32	36.450894868159665	38.0	35.0	40.0	30.0	41.0
33	36.435310643123756	38.0	35.0	40.0	30.0	41.0
34	36.434399658116824	38.0	35.0	40.0	30.0	41.0
35	36.32126592245272	38.0	35.0	40.0	30.0	41.0
36	36.257939651388696	38.0	35.0	40.0	30.0	41.0
37	36.12051231224124	38.0	35.0	40.0	30.0	41.0
38	36.08034807365362	38.0	35.0	40.0	29.0	41.0
39	35.97938299734166	38.0	35.0	40.0	29.0	41.0
40	35.923000663014186	38.0	35.0	40.0	29.0	41.0
41	35.860037429273675	38.0	35.0	40.0	29.0	41.0
42	35.82562877687575	38.0	35.0	40.0	28.0	41.0
43	34.51855899557496	37.0	33.0	40.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	5.0
11	7.0
12	2.0
13	2.0
14	6.0
15	9.0
16	18.0
17	50.0
18	98.0
19	282.0
20	622.0
21	1318.0
22	2639.0
23	4780.0
24	8593.0
25	14404.0
26	22145.0
27	33941.0
28	48837.0
29	68887.0
30	92999.0
31	122041.0
32	158889.0
33	206037.0
34	268516.0
35	353506.0
36	473471.0
37	654347.0
38	927334.0
39	516520.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.05715790264419	18.866182935135406	13.02376927206871	26.052889890151686
2	18.325043771749264	20.977401994368776	35.71689319441943	24.980661039462536
3	19.956098863730862	22.693023427589882	30.239275513170217	27.11160219550904
4	15.192471334497565	15.856565837760755	34.08282325961289	34.86813956812879
5	15.36941748463121	35.65689782220316	33.917534501735666	15.056150191429957
6	35.05425586518829	34.207763371016355	15.353614683490493	15.384366080304861
7	28.784588726447485	30.46609218836637	21.529369468234485	19.219949616951656
8	26.06728576464077	35.15407228638394	19.817943691278085	18.96069825769721
9	26.457532044638764	14.958318541760724	19.917182267598957	38.666967146001554
10	17.12076480532783	26.678419528945884	33.32489177342351	22.87592389230278
11	35.314235811458765	21.96036637374956	21.64498869057085	21.080409124220818
12	21.098849912833355	24.884311687515563	30.67389023007522	23.342948169575862
13	30.615000300228097	20.005140307016518	24.522781785425092	24.85707760733029
14	22.006418097900486	20.876153522831277	26.06680841452682	31.05061996474141
15	24.98244482146729	27.667112109694052	22.995487534001775	24.354955534836886
16	24.011188081723343	26.388994617751848	25.15667761305849	24.44313968746632
17	23.654758288745064	26.35746438654104	25.960283968045683	24.027493356668217
18	23.645336904917134	25.696409849792996	26.736028150592407	23.92222509469747
19	24.643903095916972	25.437258985299376	27.03293992146837	22.885897997315283
20	24.197831976277207	25.187001907139322	26.89272460641855	23.72244151016492
21	23.78502462247259	25.550968807179945	27.278046643135816	23.385959927211644
22	24.382465975614444	25.0583434895851	26.50602076673985	24.053169768060602
23	23.79638053044652	25.456880587351677	26.70077961323084	24.04595926897096
24	23.72324546825157	25.3273930880206	26.80207833214875	24.147283111579082
25	24.153111807707294	25.09932022831405	26.57106600068789	24.17650196329077
26	23.681916997859712	25.499842097607043	26.87209805675793	23.94614284777531
27	24.010936844821266	25.802783554132887	26.45999416627913	23.726285434766716
28	23.729626885564358	25.928954726356533	26.293775831864224	24.047642556214885
29	23.733621552307397	26.029148002905306	26.78268284330832	23.454547601478982
30	24.200369468988196	25.66563332928842	26.434945847141943	23.699051354581442
31	23.903708935014308	25.304430035170654	26.871771448785232	23.920089581029806
32	23.51064880171303	25.7208552003652	26.6816856086729	24.086810389248868
33	23.511176399207397	25.185720598938726	26.737887303667783	24.565215698186094
34	23.829694543662082	25.165948254745174	27.186747152920614	23.817610048672126
35	23.652522280316568	25.09148163696921	27.061229196642365	24.194766886071854
36	23.448970142252847	24.845545833524902	27.434491862059886	24.270992162162365
37	23.156605759304497	24.562527463333858	27.85483632292685	24.426030454434798
38	23.180322522860674	24.35256878426714	28.06291072522798	24.404197967644205
39	22.68108967474117	24.41977465557305	28.734818696145798	24.164316973539982
40	23.100278445858574	24.163688881284788	28.666004908666594	24.07002776419005
41	22.430380370157376	24.415654370378967	28.774639745125185	24.37932551433847
42	22.102340347113927	24.690733654464342	28.75064662097672	24.456279377445007
43	21.895723118844852	24.470449138722213	28.565711137356992	25.06811660507594
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3458.0
1	2988.0
2	2518.0
3	3977.0
4	5436.0
5	5436.0
6	6311.5
7	7187.0
8	6627.0
9	6067.0
10	8495.5
11	10924.0
12	10924.0
13	16866.0
14	22808.0
15	27820.5
16	32833.0
17	30754.0
18	28675.0
19	28675.0
20	32879.0
21	37083.0
22	33090.0
23	29097.0
24	33998.0
25	38899.0
26	38899.0
27	46138.0
28	53377.0
29	61390.0
30	69403.0
31	80006.5
32	90610.0
33	90610.0
34	104301.0
35	117992.0
36	134198.5
37	150405.0
38	168057.0
39	185709.0
40	185709.0
41	204839.5
42	223970.0
43	245138.5
44	266307.0
45	286980.5
46	307654.0
47	307654.0
48	326091.0
49	344528.0
50	351687.0
51	358846.0
52	352949.5
53	347053.0
54	347053.0
55	328829.5
56	310606.0
57	287409.5
58	264213.0
59	239552.5
60	214892.0
61	214892.0
62	187561.5
63	160231.0
64	135420.0
65	110609.0
66	90706.5
67	70804.0
68	70804.0
69	57500.5
70	44197.0
71	35536.0
72	26875.0
73	21726.0
74	16577.0
75	16577.0
76	13168.5
77	9760.0
78	7681.0
79	5602.0
80	4202.5
81	2803.0
82	2803.0
83	2089.5
84	1376.0
85	985.5
86	595.0
87	412.0
88	229.0
89	229.0
90	150.5
91	72.0
92	44.5
93	17.0
94	12.0
95	7.0
96	7.0
97	5.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3980307.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.728838072043956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.64143334603189	49.57989385640741
2	9.555674273370427	11.60609991233418
3	3.3158774326044784	6.041081510141468
4	1.6214582707561584	3.938771070613086
5	0.9249621192548164	2.808593738150018
6	0.6257577707748124	2.280092540022407
7	0.41153694143849107	1.7494512194097656
8	0.31457302804149617	1.5282923585411652
9	0.22958262017774206	1.2548057188436703
>10	1.2892462229117323	13.847102597309682
>50	0.053390127597801706	2.1843779151291094
>100	0.015680240175038416	1.4982434633171389
>500	3.724230941749666E-4	0.15668431485999634
>1k	2.896623997021479E-4	0.3654878312929599
>5k	4.138034281458796E-5	0.18347267988653448
>10k+	1.2414102844376387E-4	0.9775492737414314
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15051	0.378136661317833	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13601	0.34170731051650033	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10248	0.25746757724969455	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7301	0.1834280622072619	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.0247380415631256E-5
2	0.0	0.0	0.0	0.0	5.0247380415631256E-5
3	0.0	0.0	0.0	0.0	5.0247380415631256E-5
4	0.0	0.0	0.0	2.5123690207815628E-5	5.0247380415631256E-5
5	0.0	0.0	0.0	2.5123690207815628E-5	5.0247380415631256E-5
6	0.0	0.0	0.0	2.5123690207815628E-5	1.758658314547094E-4
7	0.0	0.0	0.0	2.5123690207815628E-5	1.758658314547094E-4
8	0.0	0.0	0.0	2.5123690207815628E-5	2.0098952166252502E-4
9	0.0	0.0	0.0	2.5123690207815628E-5	2.2611321187034064E-4
10	0.0	0.0	0.0	2.5123690207815628E-5	2.5123690207815626E-4
11	5.0247380415631256E-5	0.0	0.0	2.5123690207815628E-5	2.5123690207815626E-4
12	5.0247380415631256E-5	0.0	0.0	2.5123690207815628E-5	2.5123690207815626E-4
13	5.0247380415631256E-5	0.0	0.0	2.5123690207815628E-5	2.5123690207815626E-4
14	7.537107062344689E-5	0.0	0.0	2.5123690207815628E-5	2.763605922859719E-4
15	7.537107062344689E-5	0.0	0.0	7.537107062344689E-5	3.7685535311723445E-4
16	7.537107062344689E-5	0.0	0.0	7.537107062344689E-5	4.271027335328657E-4
17	7.537107062344689E-5	0.0	0.0	1.5074214124689378E-4	4.271027335328657E-4
18	7.537107062344689E-5	0.0	0.0	2.0098952166252502E-4	4.522264237406813E-4
19	1.0049476083126251E-4	0.0	0.0	3.0148428249378756E-4	4.522264237406813E-4
20	1.0049476083126251E-4	0.0	0.0	5.275974943641282E-4	4.522264237406813E-4
21	1.2561845103907813E-4	0.0	0.0	8.290817768579157E-4	4.7735011394849693E-4
22	1.2561845103907813E-4	0.0	0.0	0.0011808134397673345	5.024738041563125E-4
23	1.2561845103907813E-4	0.0	0.0	0.001482297722261122	6.029685649875751E-4
24	1.2561845103907813E-4	0.0	0.0	0.0024621216403659316	6.280922551953907E-4
25	1.2561845103907813E-4	0.0	0.0	0.003969543052834869	6.280922551953907E-4
26	1.2561845103907813E-4	0.0	0.0	0.006934138497357113	6.280922551953907E-4
27	1.2561845103907813E-4	0.0	0.0	0.028414893625039475	6.280922551953907E-4
28	1.2561845103907813E-4	0.0	0.0	0.0906462742697988	6.280922551953907E-4
29	1.2561845103907813E-4	0.0	0.0	0.17493625491702022	6.280922551953907E-4
30	1.2561845103907813E-4	0.0	0.0	0.2827922569791727	6.280922551953907E-4
31	1.2561845103907813E-4	0.0	0.0	0.6070888501816568	6.78339635611022E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5180	0.0	19.5	1
CTTATAC	2820	0.0	16.531914	37
TCTTATA	4780	0.0	14.861925	37
GTATCAA	7235	0.0	14.06358	2
TAGTACT	385	0.0	13.454545	4
CGTATAC	175	5.3143594E-7	12.685714	3
CTGTGCG	685	0.0	12.423358	9
TATACTG	650	0.0	12.2384615	5
CTCTTAT	7335	0.0	12.156782	37
CTAGTAC	215	4.020694E-8	12.046512	3
TCGTATA	155	2.7236314E-5	11.935484	2
CGTATTA	125	7.225567E-4	11.84	28
TATACCG	175	7.344761E-6	11.628571	5
GAGTGTC	1455	0.0	11.570446	7
ACCGTCC	385	0.0	11.532467	8
GTCGTAT	195	1.9635154E-6	11.384616	1
TCTATAC	440	0.0	11.352273	3
GTGTACG	245	2.0187144E-8	11.326531	1
TCGTTAC	595	0.0	11.193277	23
CTCGTTA	645	0.0	11.186047	22
>>END_MODULE