##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727143.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4108959
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.604683570704893	31.0	31.0	34.0	30.0	34.0
2	31.856693873071013	31.0	31.0	34.0	30.0	34.0
3	31.97067408070998	33.0	31.0	34.0	30.0	34.0
4	35.69408236976811	37.0	35.0	37.0	33.0	37.0
5	35.30437417360456	37.0	35.0	37.0	33.0	37.0
6	35.31747286843213	37.0	35.0	37.0	32.0	37.0
7	35.272451246167215	37.0	35.0	37.0	32.0	37.0
8	35.22126942614906	37.0	35.0	37.0	32.0	37.0
9	37.04673397811952	39.0	37.0	39.0	33.0	39.0
10	36.80847241357239	39.0	37.0	39.0	33.0	39.0
11	36.926523968722975	39.0	37.0	39.0	33.0	39.0
12	36.81320913642604	39.0	37.0	39.0	33.0	39.0
13	36.94250198164547	39.0	37.0	39.0	33.0	39.0
14	37.978231712703874	40.0	37.0	41.0	33.0	41.0
15	37.9832035316001	40.0	37.0	41.0	33.0	41.0
16	37.92080646217205	39.0	37.0	41.0	33.0	41.0
17	37.850015295845004	39.0	37.0	41.0	33.0	41.0
18	37.865382691820486	39.0	37.0	41.0	33.0	41.0
19	37.9137036899127	40.0	37.0	41.0	33.0	41.0
20	37.791678135508285	39.0	37.0	41.0	33.0	41.0
21	37.780876372823386	39.0	37.0	41.0	33.0	41.0
22	37.69032740409432	39.0	37.0	41.0	32.0	41.0
23	37.624599320655186	39.0	37.0	40.0	32.0	41.0
24	37.525682782427374	39.0	37.0	41.0	32.0	41.0
25	37.49529625386868	39.0	36.0	40.0	32.0	41.0
26	37.35368520347855	39.0	36.0	40.0	32.0	41.0
27	37.1650880429812	39.0	36.0	40.0	31.0	41.0
28	37.05529186346225	39.0	36.0	40.0	31.0	41.0
29	36.97197635702863	39.0	36.0	40.0	31.0	41.0
30	36.92295226114449	39.0	36.0	40.0	31.0	41.0
31	36.87565853054265	39.0	36.0	40.0	31.0	41.0
32	36.76288179074067	39.0	35.0	40.0	30.0	41.0
33	36.73405721497829	39.0	35.0	40.0	30.0	41.0
34	36.725582075654685	39.0	35.0	40.0	30.0	41.0
35	36.62672978727702	39.0	35.0	40.0	30.0	41.0
36	36.55019556048138	39.0	35.0	40.0	30.0	41.0
37	36.42619700026211	38.0	35.0	40.0	30.0	41.0
38	36.38337058121047	38.0	35.0	40.0	30.0	41.0
39	36.26957849907969	38.0	35.0	40.0	30.0	41.0
40	36.21285853667559	38.0	35.0	40.0	30.0	41.0
41	36.158524580070036	38.0	35.0	40.0	30.0	41.0
42	36.105032442523765	38.0	35.0	40.0	29.0	41.0
43	34.79319579484731	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	7.0
11	7.0
12	2.0
13	7.0
14	5.0
15	8.0
16	12.0
17	33.0
18	89.0
19	236.0
20	551.0
21	1126.0
22	2182.0
23	4178.0
24	7287.0
25	12210.0
26	19011.0
27	29517.0
28	43321.0
29	61697.0
30	84219.0
31	112832.0
32	147652.0
33	194029.0
34	255620.0
35	342641.0
36	471433.0
37	664972.0
38	992012.0
39	662063.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.96291566793439	18.948400312585257	13.136417277466142	25.952266742014217
2	18.050240949106573	21.172369936034894	36.29892632172771	24.478462793130813
3	19.602799638545918	23.04982843586417	30.670250056036092	26.677121869553822
4	14.843297292574592	16.06311963687153	34.50178013457909	34.59180293597478
5	15.071432934716555	36.06429268337796	34.09223601403665	14.772038367868845
6	34.28853877587973	34.539745955119045	15.699815938781574	15.47189933021965
7	28.252484388381582	31.08074332209204	21.82837550824917	18.838396781277204
8	25.805173524486374	35.590450038562075	20.084065088018644	18.520311348932907
9	26.81267931853299	15.0890529693774	20.144567030238072	37.95370068185153
10	16.933121016783083	27.01141578682094	33.39123120965675	22.66423198673922
11	35.01743385611781	22.283137894537276	21.8481372045815	20.851291044763407
12	21.041460866365423	25.121691406509534	30.789063604674567	23.04778412245048
13	30.33420387012866	20.45418316415423	24.821956120759538	24.389656844957567
14	21.756848875834486	21.23939421152657	26.572910559584557	30.430846353054385
15	24.91677332385161	27.781197135332818	23.480010387059107	23.822019153756465
16	23.47808775896766	26.804842783780515	25.780082011039777	23.936987446212047
17	23.457644624830767	26.533143796275404	26.13304245673904	23.876169122154785
18	23.296070853955953	25.977187896009667	27.150794154918557	23.57594709511582
19	24.593066029619667	25.572876244323684	27.483749533641	22.350308192415646
20	23.74421355871402	25.319478729283983	27.399908346615288	23.536399365386707
21	23.346789296267012	25.950465799245016	28.051752280808838	22.650992623679137
22	24.09761207157336	25.2267545137345	26.86828951079823	23.80734390389391
23	23.328779868574983	25.642212540937987	27.377907640353676	23.651099950133357
24	23.18166231398269	25.494900289830102	27.517334682580184	23.80610271360702
25	23.64289835941415	25.319138010381703	27.143152316681668	23.894811313522478
26	23.207216231653806	25.671246658825265	27.413488428577654	23.708048680943275
27	23.83445539368974	26.08040138633654	26.86782710657371	23.217316113400013
28	23.242894368135577	26.27497621660377	26.586320282095784	23.89580913316487
29	23.22683190559945	26.32620573726825	27.394359495920988	23.05260286121132
30	23.98887406761664	25.78684771495651	26.841397054582437	23.38288116284441
31	23.560176677353073	25.39947466012681	27.456784066231858	23.583564596288255
32	22.994826670210145	26.093032322785408	27.18075308125489	23.731387925749566
33	23.058273397227865	25.233836599489067	27.34751064685727	24.360379356425803
34	23.478550163192185	25.366765645507776	27.976453403404612	23.178230787895426
35	23.323839444491902	25.292780969583777	27.572506807685354	23.810872778238963
36	22.97494328855557	24.99319170622048	28.080859409889463	23.951005595334486
37	22.642547662315444	24.75648941739258	28.46492262395415	24.136040296337832
38	22.895020368906092	24.315915539678056	28.658207589805595	24.13085650161026
39	22.19637625977772	24.557631263782383	29.457826179331555	23.788166297108344
40	22.82181447904445	24.157262216537084	29.357800844447464	23.663122459971007
41	22.031395299880092	24.4333662127074	29.518303784486534	24.016934702925973
42	21.613722599811776	24.802754176909527	29.434340912138573	24.149182311140123
43	21.199262392250688	24.507837629920378	29.219614992507836	25.0732849853211
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6585.0
1	5273.5
2	3962.0
3	6042.0
4	8122.0
5	8122.0
6	8925.0
7	9728.0
8	8722.5
9	7717.0
10	10569.5
11	13422.0
12	13422.0
13	20527.5
14	27633.0
15	32410.0
16	37187.0
17	35308.0
18	33429.0
19	33429.0
20	38676.0
21	43923.0
22	39387.0
23	34851.0
24	40832.0
25	46813.0
26	46813.0
27	55138.0
28	63463.0
29	71908.5
30	80354.0
31	92142.0
32	103930.0
33	103930.0
34	116616.0
35	129302.0
36	144852.5
37	160403.0
38	176934.5
39	193466.0
40	193466.0
41	213065.5
42	232665.0
43	251984.5
44	271304.0
45	293117.5
46	314931.0
47	314931.0
48	334012.0
49	353093.0
50	361375.0
51	369657.0
52	360414.0
53	351171.0
54	351171.0
55	328778.5
56	306386.0
57	283072.0
58	259758.0
59	234722.0
60	209686.0
61	209686.0
62	182200.5
63	154715.0
64	129951.0
65	105187.0
66	86844.0
67	68501.0
68	68501.0
69	55414.0
70	42327.0
71	34060.5
72	25794.0
73	20787.0
74	15780.0
75	15780.0
76	12926.0
77	10072.0
78	8100.0
79	6128.0
80	4848.0
81	3568.0
82	3568.0
83	2872.0
84	2176.0
85	1675.5
86	1175.0
87	825.0
88	475.0
89	475.0
90	286.5
91	98.0
92	56.5
93	15.0
94	9.0
95	3.0
96	3.0
97	2.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4108959.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.41557301774177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18765224205889	47.42623227685018
2	9.980441423323617	11.660264094269108
3	3.2512803120423617	5.697762074077704
4	1.586552641718362	3.7071752675516025
5	0.9356869671199886	2.732934517477352
6	0.600044303368571	2.1031159090384053
7	0.4314961182762436	1.7644265102826633
8	0.3407225881817307	1.5922804182979078
9	0.23255473632397666	1.2226336362319452
>10	1.3536638061425235	14.210561103395802
>50	0.07054554205967935	2.7793732272597995
>100	0.027814201111641026	2.777389422476654
>500	8.769590642177943E-4	0.3392761567985696
>1k	5.011194061078849E-4	0.6524015438255646
>5k	4.175995050898868E-5	0.2261840829475963
>10k+	1.2527985152696605E-4	1.1079897592191352
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17515	0.42626368381869956	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15417	0.37520452260536064	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12488	0.30392126083516535	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9272	0.2256532615681977	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.867412889736792E-5
2	0.0	0.0	0.0	0.0	4.867412889736792E-5
3	0.0	0.0	0.0	2.433706444868396E-5	4.867412889736792E-5
4	0.0	0.0	0.0	2.433706444868396E-5	4.867412889736792E-5
5	0.0	0.0	0.0	2.433706444868396E-5	4.867412889736792E-5
6	0.0	0.0	0.0	4.867412889736792E-5	7.301119334605188E-5
7	0.0	0.0	0.0	4.867412889736792E-5	7.301119334605188E-5
8	0.0	0.0	0.0	4.867412889736792E-5	7.301119334605188E-5
9	0.0	0.0	0.0	4.867412889736792E-5	9.734825779473584E-5
10	0.0	0.0	0.0	7.301119334605188E-5	9.734825779473584E-5
11	0.0	0.0	0.0	7.301119334605188E-5	9.734825779473584E-5
12	0.0	0.0	0.0	9.734825779473584E-5	9.734825779473584E-5
13	2.433706444868396E-5	0.0	0.0	9.734825779473584E-5	9.734825779473584E-5
14	2.433706444868396E-5	0.0	0.0	1.216853222434198E-4	2.920447733842075E-4
15	4.867412889736792E-5	0.0	0.0	1.216853222434198E-4	3.650559667302594E-4
16	4.867412889736792E-5	0.0	0.0	2.1903358003815565E-4	3.650559667302594E-4
17	4.867412889736792E-5	0.0	0.0	2.6770770893552357E-4	3.650559667302594E-4
18	4.867412889736792E-5	0.0	0.0	3.4071890228157546E-4	4.380671600763113E-4
19	4.867412889736792E-5	0.0	0.0	4.380671600763113E-4	4.380671600763113E-4
20	4.867412889736792E-5	0.0	0.0	6.57100740114467E-4	4.380671600763113E-4
21	4.867412889736792E-5	0.0	0.0	9.491455134986744E-4	4.867412889736792E-4
22	4.867412889736792E-5	0.0	0.0	0.0015575721247157734	5.110783534223632E-4
23	4.867412889736792E-5	0.0	0.0	0.0022876840581762922	5.84089546768415E-4
24	4.867412889736792E-5	0.0	0.0	0.0037965820539946978	5.84089546768415E-4
25	4.867412889736792E-5	0.0	0.0	0.005451502436505207	6.32763675665783E-4
26	4.867412889736792E-5	0.0	0.0	0.00958880339278148	6.57100740114467E-4
27	4.867412889736792E-5	0.0	0.0	0.035726810610668056	6.57100740114467E-4
28	4.867412889736792E-5	0.0	0.0	0.10866499276337388	6.57100740114467E-4
29	4.867412889736792E-5	0.0	0.0	0.20968814728986102	6.57100740114467E-4
30	4.867412889736792E-5	0.0	0.0	0.33378283891370053	6.57100740114467E-4
31	4.867412889736792E-5	0.0	0.0	0.72324888128599	6.57100740114467E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5525	0.0	22.769232	1
CTTATAC	3425	0.0	17.3927	37
GTATCAA	8055	0.0	15.479827	2
TCTTATA	5510	0.0	14.77314	37
TCGTTAC	885	0.0	14.423728	23
CTCGTTA	975	0.0	14.230769	22
GGACCGT	265	1.8189894E-12	13.264152	6
TACTTAC	1905	0.0	13.207349	31
GTACTAC	240	8.185452E-11	13.104167	1
CCGCGTA	85	0.009410121	13.058824	9
CTCTTAT	8485	0.0	12.427814	37
TAATACG	105	0.0026455673	12.333333	4
AGTGTAC	2295	0.0	12.333333	27
GTACTTA	2170	0.0	12.191244	30
TACGGAT	1510	0.0	12.12914	27
GTGTACT	2230	0.0	12.112107	28
GGAGTGT	2170	0.0	12.020738	6
ACGGATG	1540	0.0	12.012986	28
ATACCGA	170	5.3173153E-6	11.970589	6
ATACCCG	125	7.2256546E-4	11.84	5
>>END_MODULE