FastQCFastQC Report
Thu 9 Feb 2017
SRR2727142.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727142.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4952290
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT132850.26825973438550654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT113140.2284599649858954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91290.18433896237902062No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66220.13371591728271165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35000.025.0542871
GTATCAA52300.016.872852
CTTATAC37600.015.597074537
TCTTATA57050.014.33304137
TACTTAC15150.011.84488531
TATCAAC76550.011.7935983
TAGGCGG1901.4364578E-611.6842115
GTACTTA16150.011.56965930
TCAACGC76050.011.5305725
AGTGTAC17500.011.52285827
TCGTTAC6950.011.44604323
ATCAACG76950.011.4437944
CTCTTAT84750.011.41651937
TATACTG7950.011.4025165
CAACGCA78350.011.1684756
TAGATCG1350.001376366110.9629635
GGAGTGT18600.010.940866
TCTCTTA119300.010.7309337
TTAATAC8350.010.634733
AACGCAG82500.010.629097
Produced by FastQC (version 0.11.5)