##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727142.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4952290
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.611563337365137	31.0	31.0	34.0	30.0	34.0
2	31.863217622554416	31.0	31.0	34.0	30.0	34.0
3	31.98049124748349	33.0	31.0	34.0	30.0	34.0
4	35.70639926175567	37.0	35.0	37.0	33.0	37.0
5	35.306903472938785	37.0	35.0	37.0	33.0	37.0
6	35.31599663993829	37.0	35.0	37.0	32.0	37.0
7	35.270570584517465	37.0	35.0	37.0	32.0	37.0
8	35.21982315252136	37.0	35.0	37.0	32.0	37.0
9	37.0552617072102	39.0	37.0	39.0	33.0	39.0
10	36.82152923193109	39.0	37.0	39.0	33.0	39.0
11	36.93917500792563	39.0	37.0	39.0	33.0	39.0
12	36.817319866162926	39.0	37.0	39.0	33.0	39.0
13	36.944207629197805	39.0	37.0	39.0	33.0	39.0
14	38.00368980814936	40.0	37.0	41.0	33.0	41.0
15	38.00631021204332	40.0	37.0	41.0	33.0	41.0
16	37.95469913918611	40.0	37.0	41.0	33.0	41.0
17	37.857836273723876	39.0	37.0	41.0	33.0	41.0
18	37.873060543708064	39.0	37.0	41.0	33.0	41.0
19	37.92036997025618	40.0	37.0	41.0	33.0	41.0
20	37.800313592297705	39.0	37.0	41.0	33.0	41.0
21	37.79349250548736	39.0	37.0	41.0	33.0	41.0
22	37.70387477308478	39.0	37.0	41.0	32.0	41.0
23	37.649740625044174	39.0	37.0	40.0	32.0	41.0
24	37.5474479483229	39.0	37.0	41.0	32.0	41.0
25	37.52760238192836	39.0	37.0	40.0	32.0	41.0
26	37.396252036936446	39.0	36.0	40.0	32.0	41.0
27	37.21893770356744	39.0	36.0	40.0	31.0	41.0
28	37.11993461610689	39.0	36.0	40.0	31.0	41.0
29	37.04497777795727	39.0	36.0	40.0	31.0	41.0
30	36.998691514430696	39.0	36.0	40.0	31.0	41.0
31	36.96982991706867	39.0	36.0	40.0	31.0	41.0
32	36.87869127211856	39.0	36.0	40.0	30.0	41.0
33	36.86194831078148	39.0	36.0	40.0	30.0	41.0
34	36.86185158785128	39.0	36.0	40.0	30.0	41.0
35	36.77858223165445	39.0	35.0	40.0	30.0	41.0
36	36.705011418959714	39.0	35.0	40.0	30.0	41.0
37	36.58148856387651	39.0	35.0	40.0	30.0	41.0
38	36.54709901883775	39.0	35.0	40.0	30.0	41.0
39	36.45691407409502	38.0	35.0	40.0	30.0	41.0
40	36.40768977584108	38.0	35.0	40.0	30.0	41.0
41	36.36185603024055	38.0	35.0	40.0	30.0	41.0
42	36.31427258904466	38.0	35.0	40.0	30.0	41.0
43	34.98034707175872	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	3.0
12	4.0
13	3.0
14	2.0
15	9.0
16	17.0
17	42.0
18	80.0
19	223.0
20	524.0
21	1174.0
22	2311.0
23	4480.0
24	7922.0
25	13082.0
26	21137.0
27	33006.0
28	49214.0
29	71045.0
30	98495.0
31	132349.0
32	174491.0
33	229186.0
34	305494.0
35	408753.0
36	562505.0
37	802562.0
38	1217548.0
39	816625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.19395471589911	18.658196511109	12.678175147255107	26.469673625736778
2	18.293112883130835	20.56337573122737	35.88418691150962	25.259324474132168
3	19.770792905908177	22.367591558652663	29.99341718679641	27.868198348642746
4	15.040355068059421	15.604982745356189	34.06674487964154	35.28791730694285
5	15.43433441902635	35.465249409868974	33.970163298191345	15.130252872913339
6	35.32319391634981	34.119912202233714	15.33835861793231	15.218535263484165
7	29.15947975583013	30.21438566804448	21.34194483764077	19.28418973848462
8	26.120764333268042	35.13950111968403	19.742745275418038	18.996989271629893
9	26.5956557471392	14.904397763458926	19.703450322981894	38.79649616641998
10	17.095242806863087	26.49852896336846	33.38770548574498	23.01852274402347
11	35.685672688796494	21.69105605689489	21.59524179722916	21.02802945707945
12	21.30212487556262	24.523079221935713	30.651759085190893	23.52303681731078
13	30.61418051043053	19.91961294673777	24.373855327535342	25.09235121529636
14	22.202354870171174	20.544798466971844	25.701281629306845	31.551565033550133
15	25.023817264336294	27.47646038499361	22.70062940579005	24.799092944880044
16	24.39731921999721	26.244081021103366	24.7597979924439	24.59880176645552
17	23.980259637460648	26.178131732996253	25.63922952815768	24.202379101385418
18	24.012305418301434	25.562335808282633	26.34472133093983	24.080637442476107
19	24.903105432032454	25.283656651771203	26.51987262458378	23.293365291612567
20	24.41343297747103	25.03336840128506	26.50686853960491	24.046330081639
21	24.06385732661052	25.37880455304516	26.824115712125096	23.73322240821923
22	24.719069359831515	24.898885162217884	26.074401943343382	24.307643534607223
23	24.134127847924898	25.200583972263335	26.237801098077856	24.427487081733908
24	24.01117462830327	25.18842797978309	26.287414509247238	24.512982882666403
25	24.49272962609217	24.914473910049693	26.078743369229183	24.51405309462895
26	24.065715053036072	25.270834300899182	26.302861908329277	24.360588737735473
27	24.402751050524103	25.587415115027596	25.933234927679923	24.076598906768385
28	24.152462800037963	25.557550143468983	25.84967762388713	24.440309432605925
29	24.091743415672344	25.73153026175769	26.31624965420038	23.860476668369582
30	24.50442118696603	25.432436307243723	25.970954851190054	24.092187654600195
31	24.265117753604898	24.983149209759524	26.41892942456924	24.332803612066336
32	23.81312483719653	25.457858889523838	26.28083169604365	24.448184577235985
33	23.838547419476647	24.981877071011592	26.344398248083213	24.83517726142855
34	24.270428428060555	24.91205078862506	26.71251077784217	24.105010005472217
35	23.988195360126323	24.896684160257173	26.663684881135797	24.451435598480703
36	23.82025285272066	24.599286390740446	26.927865694456504	24.65259506208239
37	23.581211924180533	24.256858948082606	27.40249460350666	24.759434524230205
38	23.597022791476267	24.099315670124327	27.62225152404241	24.681410014356995
39	23.103271415850042	24.127141181150538	28.26692297906625	24.502664423933172
40	23.444063251546254	23.895268653491616	28.251293845877363	24.409374249084767
41	22.859081354282566	24.083726922292513	28.38202124673636	24.675170476688564
42	22.485395645246946	24.337023881880906	28.394480129394683	24.783100343477464
43	22.29746238608805	24.043745418786056	28.269911495489964	25.388880699635923
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3376.0
1	2888.0
2	2400.0
3	3926.5
4	5453.0
5	5453.0
6	6313.0
7	7173.0
8	6447.5
9	5722.0
10	7764.5
11	9807.0
12	9807.0
13	15255.5
14	20704.0
15	24503.0
16	28302.0
17	27517.0
18	26732.0
19	26732.0
20	31898.5
21	37065.0
22	35478.5
23	33892.0
24	40420.0
25	46948.0
26	46948.0
27	56071.0
28	65194.0
29	74925.5
30	84657.0
31	98178.0
32	111699.0
33	111699.0
34	128080.5
35	144462.0
36	164808.0
37	185154.0
38	206315.0
39	227476.0
40	227476.0
41	251826.0
42	276176.0
43	302357.0
44	328538.0
45	354911.0
46	381284.0
47	381284.0
48	405074.5
49	428865.0
50	439538.0
51	450211.0
52	444819.5
53	439428.0
54	439428.0
55	417821.0
56	396214.0
57	367863.5
58	339513.0
59	308795.5
60	278078.0
61	278078.0
62	243356.0
63	208634.0
64	176645.0
65	144656.0
66	118839.5
67	93023.0
68	93023.0
69	75519.5
70	58016.0
71	46383.5
72	34751.0
73	28126.5
74	21502.0
75	21502.0
76	17159.0
77	12816.0
78	10114.5
79	7413.0
80	5543.5
81	3674.0
82	3674.0
83	2813.0
84	1952.0
85	1393.0
86	834.0
87	605.0
88	376.0
89	376.0
90	233.0
91	90.0
92	57.0
93	24.0
94	15.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4952290.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.66406481979381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.74903616979012	47.37066692000441
2	9.971479472622653	11.69935036262357
3	3.4376037626879676	6.04991429877282
4	1.674405442805666	3.929097177254685
5	0.9646181722458517	2.829421149149083
6	0.6295849259355004	2.216040654278716
7	0.44129909398749273	1.8121879058218986
8	0.3303533523659123	1.550389638130405
9	0.2564549311966404	1.3540219836367924
>10	1.4509924768122124	15.246311600596348
>50	0.07384021981729368	2.8828793254592333
>100	0.019643156635485125	1.8445159347977185
>500	3.099709582025981E-4	0.1265414159212866
>1k	2.4108772480987875E-4	0.2734079882956293
>5k	6.888220708726931E-5	0.3182418876442771
>10k+	6.888220708726931E-5	0.4970117576129498
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13285	0.26825973438550654	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11314	0.2284599649858954	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9129	0.18433896237902062	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6622	0.13371591728271165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0385357077230936E-5
2	0.0	0.0	0.0	0.0	4.0385357077230936E-5
3	0.0	0.0	0.0	0.0	4.0385357077230936E-5
4	0.0	0.0	0.0	0.0	6.057803561584641E-5
5	0.0	0.0	0.0	0.0	6.057803561584641E-5
6	2.0192678538615468E-5	0.0	0.0	0.0	1.2115607123169282E-4
7	2.0192678538615468E-5	0.0	0.0	0.0	1.2115607123169282E-4
8	2.0192678538615468E-5	0.0	0.0	0.0	1.4134874977030827E-4
9	2.0192678538615468E-5	0.0	0.0	2.0192678538615468E-5	1.6154142830892375E-4
10	6.057803561584641E-5	0.0	0.0	4.0385357077230936E-5	1.6154142830892375E-4
11	6.057803561584641E-5	0.0	0.0	4.0385357077230936E-5	1.6154142830892375E-4
12	6.057803561584641E-5	0.0	0.0	4.0385357077230936E-5	1.6154142830892375E-4
13	6.057803561584641E-5	2.0192678538615468E-5	0.0	4.0385357077230936E-5	1.8173410684753922E-4
14	6.057803561584641E-5	2.0192678538615468E-5	0.0	4.0385357077230936E-5	3.230828566178475E-4
15	6.057803561584641E-5	2.0192678538615468E-5	0.0	8.077071415446187E-5	4.442389278495403E-4
16	6.057803561584641E-5	2.0192678538615468E-5	0.0	1.2115607123169282E-4	5.048169634653867E-4
17	6.057803561584641E-5	2.0192678538615468E-5	0.0	1.6154142830892375E-4	5.048169634653867E-4
18	6.057803561584641E-5	2.0192678538615468E-5	0.0	2.019267853861547E-4	5.653949990812331E-4
19	6.057803561584641E-5	2.0192678538615468E-5	0.0	2.4231214246338563E-4	5.855876776198486E-4
20	6.057803561584641E-5	2.0192678538615468E-5	0.0	2.625048210020011E-4	5.855876776198486E-4
21	6.057803561584641E-5	2.0192678538615468E-5	0.0	4.2404624931092485E-4	6.05780356158464E-4
22	6.057803561584641E-5	2.0192678538615468E-5	0.0	7.471291059287724E-4	6.46165713235695E-4
23	6.057803561584641E-5	2.0192678538615468E-5	0.0	0.0010702119625466198	8.480924986218497E-4
24	6.057803561584641E-5	2.0192678538615468E-5	0.0	0.002160616603631855	8.480924986218497E-4
25	6.057803561584641E-5	2.0192678538615468E-5	0.0	0.0033115992803329367	8.480924986218497E-4
26	6.057803561584641E-5	2.0192678538615468E-5	0.0	0.006481849810895566	8.480924986218497E-4
27	6.057803561584641E-5	2.0192678538615468E-5	0.0	0.027199537991515036	8.480924986218497E-4
28	6.057803561584641E-5	2.0192678538615468E-5	0.0	0.08646504950235144	8.480924986218497E-4
29	6.057803561584641E-5	2.0192678538615468E-5	0.0	0.16039044563222266	8.480924986218497E-4
30	6.057803561584641E-5	2.0192678538615468E-5	0.0	0.25345850101670137	8.480924986218497E-4
31	6.057803561584641E-5	2.0192678538615468E-5	0.0	0.5544909526703807	8.480924986218497E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3500	0.0	25.054287	1
GTATCAA	5230	0.0	16.87285	2
CTTATAC	3760	0.0	15.5970745	37
TCTTATA	5705	0.0	14.333041	37
TACTTAC	1515	0.0	11.844885	31
TATCAAC	7655	0.0	11.793598	3
TAGGCGG	190	1.4364578E-6	11.684211	5
GTACTTA	1615	0.0	11.569659	30
TCAACGC	7605	0.0	11.530572	5
AGTGTAC	1750	0.0	11.522858	27
TCGTTAC	695	0.0	11.446043	23
ATCAACG	7695	0.0	11.443794	4
CTCTTAT	8475	0.0	11.416519	37
TATACTG	795	0.0	11.402516	5
CAACGCA	7835	0.0	11.168475	6
TAGATCG	135	0.0013763661	10.962963	5
GGAGTGT	1860	0.0	10.94086	6
TCTCTTA	11930	0.0	10.73093	37
TTAATAC	835	0.0	10.63473	3
AACGCAG	8250	0.0	10.62909	7
>>END_MODULE