##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727141.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8198826
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00470201465429	33.0	31.0	34.0	30.0	34.0
2	32.230277359221915	34.0	31.0	34.0	30.0	34.0
3	32.34408279917149	34.0	31.0	34.0	30.0	34.0
4	35.95717240492724	37.0	35.0	37.0	35.0	37.0
5	35.66333509212173	37.0	35.0	37.0	33.0	37.0
6	35.675509274132665	37.0	35.0	37.0	33.0	37.0
7	35.643444439484384	37.0	35.0	37.0	33.0	37.0
8	35.607761037982755	37.0	35.0	37.0	33.0	37.0
9	37.431233569293944	39.0	37.0	39.0	34.0	39.0
10	37.25076419477618	39.0	37.0	39.0	34.0	39.0
11	37.33950690013424	39.0	37.0	39.0	34.0	39.0
12	37.246739106306194	39.0	37.0	39.0	34.0	39.0
13	37.31840558636078	39.0	37.0	39.0	34.0	39.0
14	38.51957121665956	40.0	38.0	41.0	34.0	41.0
15	38.47289099683296	40.0	38.0	41.0	34.0	41.0
16	38.432037342907385	40.0	38.0	41.0	34.0	41.0
17	38.38288225655722	40.0	38.0	41.0	34.0	41.0
18	38.38207604357014	40.0	38.0	41.0	34.0	41.0
19	38.43272475839834	40.0	38.0	41.0	34.0	41.0
20	38.351437510687504	40.0	38.0	41.0	34.0	41.0
21	38.29790143125369	40.0	38.0	41.0	34.0	41.0
22	38.24277439233373	40.0	38.0	41.0	34.0	41.0
23	38.184092332243665	40.0	38.0	41.0	34.0	41.0
24	38.10973717456621	40.0	37.0	41.0	33.0	41.0
25	38.07757025213122	40.0	37.0	41.0	33.0	41.0
26	37.929987415271405	40.0	37.0	41.0	33.0	41.0
27	37.806397891600575	40.0	37.0	41.0	33.0	41.0
28	37.733800912472105	40.0	37.0	41.0	33.0	41.0
29	37.64969813970927	40.0	37.0	41.0	32.0	41.0
30	37.61075329565477	40.0	37.0	41.0	32.0	41.0
31	37.57039361489072	39.0	37.0	41.0	32.0	41.0
32	37.474221431214666	39.0	37.0	41.0	32.0	41.0
33	37.41230110262128	39.0	37.0	41.0	32.0	41.0
34	37.38220923337073	39.0	36.0	41.0	32.0	41.0
35	37.31999630190957	39.0	36.0	41.0	31.0	41.0
36	37.21596274881306	39.0	36.0	41.0	31.0	41.0
37	37.14347651724771	39.0	36.0	41.0	31.0	41.0
38	37.115231863683896	39.0	36.0	41.0	31.0	41.0
39	37.02584406596749	39.0	36.0	40.0	31.0	41.0
40	36.957860796167644	39.0	36.0	40.0	31.0	41.0
41	36.91885606549035	39.0	36.0	40.0	31.0	41.0
42	36.80444309953645	39.0	36.0	40.0	30.0	41.0
43	35.524178095742	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	9.0
12	6.0
13	4.0
14	9.0
15	12.0
16	17.0
17	72.0
18	153.0
19	350.0
20	828.0
21	1733.0
22	3314.0
23	6397.0
24	10912.0
25	18168.0
26	28765.0
27	43563.0
28	63926.0
29	91055.0
30	125234.0
31	169215.0
32	223786.0
33	297461.0
34	399231.0
35	548849.0
36	783438.0
37	1205569.0
38	2138001.0
39	2038741.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45267554159584	18.47166899260943	12.62897883184739	26.446676633947348
2	18.296924462112017	20.456697092974043	35.983737183835835	25.262641261078112
3	19.759231382639417	22.327245388547095	30.10173651691108	27.811786711902407
4	15.036933336553307	15.550397093437523	34.146644897696326	35.26602467231284
5	15.38450748924297	35.50646153485877	34.0048928956414	15.104138080256856
6	35.2555231688049	34.10899316560688	15.35417387806498	15.281309787523236
7	29.09436058284442	30.211483936846566	21.41296814934236	19.281187330966652
8	26.109421031742837	35.02179458376114	19.803737754649266	19.065046629846762
9	26.55435058629126	14.966435438439602	19.758548358021013	38.72066561724813
10	17.168604383115337	26.436321492857633	33.308024831847874	23.08704929217915
11	35.51679228221211	21.8671307331074	21.56670967282389	21.0493673118566
12	21.204621246993167	24.620207819997646	30.52116242008307	23.65400851292612
13	30.59194084616505	19.951783340688046	24.349425149405537	25.10685066374137
14	22.179480330476583	20.58134908583253	25.692434502208002	31.546736081482884
15	25.111790395356604	27.507755378635917	22.631825580881944	24.748628645125535
16	24.35512108684829	26.2051420532647	24.83146977384323	24.608267086043785
17	23.821288560093848	26.283470340753663	25.646684049643202	24.248557049509284
18	23.931121358106637	25.642805445560136	26.29721621119902	24.128856985134213
19	24.8849530408378	25.31468529762676	26.53710421467659	23.263257446858855
20	24.372855333190387	25.147380856722656	26.430857295910414	24.04890651417654
21	24.094839919764123	25.4786112060434	26.692736252726917	23.73381262146556
22	24.71885608988409	24.90290683080724	26.02206706179641	24.356170017512262
23	24.10889071191412	25.30152487685432	26.209398760261532	24.38018565097003
24	24.027598585456015	25.174189572994965	26.26763880584854	24.530573035700478
25	24.446682976318804	25.014654537125192	26.066561236938067	24.472101249617932
26	24.02267105070897	25.355044734453447	26.34755268620166	24.27473152863593
27	24.30573596756414	25.688336354497583	25.922979704655276	24.082947973283
28	24.09464476987315	25.612849937295902	25.87743659884964	24.415068693981308
29	23.97257851307004	25.81028064261883	26.36506738891641	23.852073455394713
30	24.4457438174685	25.473939805528257	25.968217888756268	24.112098488246975
31	24.22448043171059	25.04165840328847	26.40587810986597	24.327983055134965
32	23.778123843584435	25.539778499995	26.27865257782029	24.403445078600278
33	23.817263593592546	24.973721847493778	26.34560118729194	24.863413371621743
34	24.193183267945923	24.878342338281115	26.79606811999669	24.13240627377627
35	23.958783855151943	24.820870695389804	26.682259142955346	24.53808630650291
36	23.7222133998209	24.495653402084642	27.101148383927164	24.6809848141673
37	23.503291812754657	24.061359516594205	27.608464919245755	24.826883751405386
38	23.424743981638347	23.929084481119613	27.87943054286065	24.76674099438139
39	22.932624744079213	23.963552830612578	28.559857228339762	24.543965196968447
40	23.25751272194336	23.737800997362303	28.584726642570534	24.4199596381238
41	22.611737338979996	23.97695718874873	28.71868484585476	24.692620626416513
42	22.22085210736269	24.292356003164358	28.687534044508322	24.799257844964632
43	22.02199436846202	24.01063274180962	28.572212655811942	25.39516023391642
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4639.0
1	4172.0
2	3705.0
3	6331.0
4	8957.0
5	8957.0
6	10381.0
7	11805.0
8	10528.0
9	9251.0
10	13050.0
11	16849.0
12	16849.0
13	25967.5
14	35086.0
15	41979.0
16	48872.0
17	46452.5
18	44033.0
19	44033.0
20	52335.5
21	60638.0
22	57013.0
23	53388.0
24	64078.0
25	74768.0
26	74768.0
27	90369.0
28	105970.0
29	122464.5
30	138959.0
31	162651.0
32	186343.0
33	186343.0
34	215295.0
35	244247.0
36	277574.0
37	310901.0
38	348411.5
39	385922.0
40	385922.0
41	425280.0
42	464638.0
43	507987.0
44	551336.0
45	592849.5
46	634363.0
47	634363.0
48	671512.0
49	708661.0
50	727540.5
51	746420.0
52	733254.0
53	720088.0
54	720088.0
55	685111.0
56	650134.0
57	603682.5
58	557231.0
59	506889.0
60	456547.0
61	456547.0
62	399293.0
63	342039.0
64	289100.0
65	236161.0
66	194045.5
67	151930.0
68	151930.0
69	123672.0
70	95414.0
71	76324.0
72	57234.0
73	46610.5
74	35987.0
75	35987.0
76	28708.5
77	21430.0
78	17003.0
79	12576.0
80	9542.5
81	6509.0
82	6509.0
83	4878.0
84	3247.0
85	2408.5
86	1570.0
87	1115.0
88	660.0
89	660.0
90	438.5
91	217.0
92	140.0
93	63.0
94	45.0
95	27.0
96	27.0
97	18.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8198826.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.626076309966315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.12848495281827	40.334671261562505
2	10.904651777544695	11.259287696022644
3	3.844107406810917	5.953685469831814
4	1.9936300506107192	4.11693188506684
5	1.146146890117914	2.9585533405829
6	0.7338525728302548	2.273155735511991
7	0.5126128325413686	1.8524932447174076
8	0.39144797641525386	1.6167138481436631
9	0.32120592108941437	1.4924341254037612
>10	1.8370131818760391	17.7066932490824
>50	0.13565512850845093	4.806192761778266
>100	0.04993775954129393	4.125661090845346
>500	8.27935434057924E-4	0.26588551729400084
>1k	3.310330142704371E-4	0.3809956709455041
>5k	0.0	0.0
>10k+	9.458084701772125E-5	0.8566451032109356
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22842	0.2786008630991803	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19864	0.24227858964198046	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15846	0.1932715732715879	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11624	0.1417763957912999	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.659060455728662E-5
2	0.0	0.0	0.0	0.0	3.659060455728662E-5
3	0.0	0.0	0.0	0.0	3.659060455728662E-5
4	0.0	0.0	0.0	0.0	6.0984340928811025E-5
5	0.0	0.0	0.0	0.0	6.0984340928811025E-5
6	0.0	0.0	0.0	0.0	9.757494548609764E-5
7	1.2196868185762205E-5	0.0	0.0	0.0	1.2196868185762205E-4
8	1.2196868185762205E-5	0.0	0.0	1.2196868185762205E-5	1.3416555004338427E-4
9	1.2196868185762205E-5	0.0	0.0	1.2196868185762205E-5	1.7075615460067088E-4
10	2.439373637152441E-5	0.0	0.0	1.2196868185762205E-5	1.7075615460067088E-4
11	2.439373637152441E-5	0.0	0.0	1.2196868185762205E-5	1.8295302278643308E-4
12	2.439373637152441E-5	0.0	0.0	2.439373637152441E-5	1.9514989097219528E-4
13	2.439373637152441E-5	0.0	0.0	3.659060455728662E-5	1.9514989097219528E-4
14	2.439373637152441E-5	0.0	0.0	4.878747274304882E-5	2.317404955294819E-4
15	3.659060455728662E-5	0.0	0.0	8.537807730033544E-5	2.439373637152441E-4
16	4.878747274304882E-5	0.0	0.0	1.4636241822914647E-4	2.9272483645829294E-4
17	4.878747274304882E-5	0.0	0.0	1.7075615460067088E-4	2.9272483645829294E-4
18	6.0984340928811025E-5	0.0	0.0	1.7075615460067088E-4	3.1711857282981735E-4
19	6.0984340928811025E-5	0.0	0.0	2.561342319010063E-4	3.1711857282981735E-4
20	6.0984340928811025E-5	0.0	0.0	3.781029137586284E-4	3.1711857282981735E-4
21	6.0984340928811025E-5	0.0	0.0	5.122684638020126E-4	3.293154410155795E-4
22	6.0984340928811025E-5	0.0	0.0	8.537807730033544E-4	3.293154410155795E-4
23	6.0984340928811025E-5	0.0	0.0	0.0012806711595050315	5.000715956162504E-4
24	6.0984340928811025E-5	0.0	0.0	0.002305208087109057	5.122684638020126E-4
25	6.0984340928811025E-5	0.0	0.0	0.003963982160372716	5.122684638020126E-4
26	6.0984340928811025E-5	0.0	0.0	0.008110917343531867	5.366622001735371E-4
27	6.0984340928811025E-5	0.0	0.0	0.036529620216357805	5.366622001735371E-4
28	6.0984340928811025E-5	0.0	0.0	0.1069787308573203	5.366622001735371E-4
29	6.0984340928811025E-5	0.0	0.0	0.19973591341004188	5.366622001735371E-4
30	6.0984340928811025E-5	0.0	0.0	0.316313579529557	5.366622001735371E-4
31	6.0984340928811025E-5	0.0	0.0	0.6779629181055922	5.488590683592993E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7410	0.0	23.04386	1
CTTATAC	6740	0.0	16.359049	37
GTATCAA	10840	0.0	15.701107	2
TCTTATA	10955	0.0	14.3035145	37
CTCTTAT	16670	0.0	12.684764	37
TCGTTAC	1285	0.0	12.381323	23
TATCAAC	14755	0.0	11.785835	3
ATCAACG	14545	0.0	11.765212	4
CGTGCGA	270	5.802576E-10	11.648149	10
TCAACGC	14655	0.0	11.63903	5
CAACGCA	14825	0.0	11.480606	6
AACGCAG	14885	0.0	11.334901	7
TACGGAT	2005	0.0	11.256858	27
CTCGTTA	1450	0.0	11.227586	22
TTACGGA	2070	0.0	11.171497	26
GTTACGG	1675	0.0	11.044777	25
ACGGATG	2170	0.0	10.997695	28
TCTCTTA	23865	0.0	10.852714	37
GTCTTAG	1280	0.0	10.839845	1
GGGTAAG	3945	0.0	10.832699	1
>>END_MODULE