FastQCFastQC Report
Thu 9 Feb 2017
SRR2727140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11631322
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT303350.26080440383302944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT272880.23460789753735645No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT214740.18462217794331545No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156820.13482560279906272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110550.020.6169151
CTTATAC95000.016.1242137
GTATCAA155100.014.6353972
TCTTATA149750.014.26878237
CTCTTAT228800.012.60555137
GTATAGA14200.011.7253521
TCGTTAC10350.011.2608723
TATCAAC210500.010.9505943
CTCGTTA11850.010.92826922
AACGCAG207350.010.8760557
ATCAACG209400.010.8402584
TCAACGC212350.010.6548165
TCTCTTA321650.010.646237
CAACGCA213350.010.5701896
GTACCGT7200.010.5347226
TTAGACA20450.010.4034224
TATACAC40000.010.3637
GGGTAAG33950.010.1899851
TATACTG20450.010.0415655
GTATTAC10800.09.9351851
Produced by FastQC (version 0.11.5)