##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727140.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11631322
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.911500859489575	33.0	31.0	34.0	30.0	34.0
2	32.14435917086639	33.0	31.0	34.0	30.0	34.0
3	32.26178400013343	34.0	31.0	34.0	30.0	34.0
4	35.88596300575291	37.0	35.0	37.0	35.0	37.0
5	35.58080311077279	37.0	35.0	37.0	33.0	37.0
6	35.59449880245771	37.0	35.0	37.0	33.0	37.0
7	35.55712403112905	37.0	35.0	37.0	33.0	37.0
8	35.52184850526879	37.0	35.0	37.0	33.0	37.0
9	37.33927983422693	39.0	37.0	39.0	34.0	39.0
10	37.15501789048571	39.0	37.0	39.0	33.0	39.0
11	37.24227349221353	39.0	37.0	39.0	34.0	39.0
12	37.14209046916593	39.0	37.0	39.0	33.0	39.0
13	37.220230254136204	39.0	37.0	39.0	34.0	39.0
14	38.40299967621909	40.0	38.0	41.0	34.0	41.0
15	38.35542451666285	40.0	38.0	41.0	34.0	41.0
16	38.3132634450323	40.0	38.0	41.0	34.0	41.0
17	38.26504459252353	40.0	38.0	41.0	33.0	41.0
18	38.26257883669629	40.0	38.0	41.0	33.0	41.0
19	38.30745524885305	40.0	38.0	41.0	34.0	41.0
20	38.22381643290419	40.0	38.0	41.0	34.0	41.0
21	38.181070646999544	40.0	38.0	41.0	33.0	41.0
22	38.118120107069515	40.0	38.0	41.0	33.0	41.0
23	38.06395051224616	40.0	37.0	41.0	33.0	41.0
24	37.980131837120496	40.0	37.0	41.0	33.0	41.0
25	37.95276925529188	40.0	37.0	41.0	33.0	41.0
26	37.799104693344404	40.0	37.0	41.0	33.0	41.0
27	37.66941169713984	40.0	37.0	41.0	32.0	41.0
28	37.593413371240175	39.0	37.0	41.0	32.0	41.0
29	37.498727057852925	39.0	37.0	41.0	32.0	41.0
30	37.46643502776383	39.0	37.0	41.0	32.0	41.0
31	37.429152421367064	39.0	37.0	41.0	32.0	41.0
32	37.33020433962709	39.0	36.0	41.0	31.0	41.0
33	37.267911334584326	39.0	36.0	41.0	31.0	41.0
34	37.2415403855211	39.0	36.0	41.0	31.0	41.0
35	37.175124977195196	39.0	36.0	41.0	31.0	41.0
36	37.08013139005179	39.0	36.0	40.0	31.0	41.0
37	37.01141125660523	39.0	36.0	40.0	31.0	41.0
38	36.97956259830138	39.0	36.0	40.0	31.0	41.0
39	36.89243699039542	39.0	36.0	40.0	30.0	41.0
40	36.8242934036217	39.0	36.0	40.0	30.0	41.0
41	36.78520747684571	39.0	35.0	40.0	30.0	41.0
42	36.68279487060886	39.0	35.0	40.0	30.0	41.0
43	35.40516985085616	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	20.0
11	18.0
12	11.0
13	14.0
14	11.0
15	23.0
16	49.0
17	98.0
18	225.0
19	573.0
20	1254.0
21	2861.0
22	5697.0
23	10150.0
24	17610.0
25	29368.0
26	45846.0
27	69188.0
28	100418.0
29	143048.0
30	194852.0
31	258987.0
32	340231.0
33	445530.0
34	591268.0
35	802702.0
36	1132037.0
37	1713628.0
38	2988693.0
39	2736900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34130049877392	18.629215148544592	12.651975416036112	26.377508936645377
2	18.23717028898349	20.547208649197398	36.021253645974205	25.194367415844905
3	19.737128763179285	22.438386625355225	29.946131660700303	27.878352950765183
4	14.933160650182325	15.667986837609687	34.23958170876879	35.1592708034392
5	15.418513905813974	35.44688213429222	34.03603648837166	15.098567471522154
6	35.19900833284471	34.29041857838688	15.364074694174917	15.146498394593493
7	29.13668798783148	30.044194460440526	21.540449142410466	19.278668409317532
8	25.99170584392729	35.08331211189923	19.78538639030026	19.139595653873222
9	26.340531196711776	15.014699102991043	19.872341252352914	38.77242844794427
10	17.160001244914376	26.450914178113198	33.360713425352685	23.02837115161974
11	35.45540223200768	21.910793975095867	21.649310370738597	20.98449342215786
12	21.229667616458386	24.76655706032384	30.560326676537713	23.44344864668006
13	30.5835656514367	19.972484641040804	24.413518944794067	25.030430762728432
14	22.149717805078392	20.650980172331227	25.730170654720077	31.469131367870308
15	24.895321443254687	27.63893046723322	22.67638192803879	24.789366161473307
16	24.446344104307318	26.234232015930775	24.73339659928596	24.586027280475943
17	23.77832889503016	26.40642224503801	25.712563025939783	24.102685833992044
18	23.977033737007712	25.66108994317241	26.293288071639665	24.068588248180216
19	24.792409667619896	25.41793615549462	26.546397735356308	23.243256441529176
20	24.357626759881637	25.290272249362538	26.467025846245164	23.885075144510658
21	24.095859438849683	25.502905000824498	26.61480784385472	23.786427716471096
22	24.61114910239782	25.100319636925196	26.056539402829703	24.231991857847284
23	24.121041443096495	25.356790913363074	26.143906943681895	24.378260699858536
24	24.070961151277558	25.368449089450024	26.163853085659568	24.396736673612853
25	24.458948002643208	25.10535775726955	26.05987522312597	24.37581901696127
26	24.050395991100583	25.457097654075778	26.275207581734904	24.217298773088732
27	24.26351879863699	25.74351393590514	25.928858301747642	24.06410896371023
28	24.11405169592932	25.5615913651088	26.05872316147726	24.265633777484624
29	24.024079120155044	25.83045160300781	26.30195432642996	23.843514950407187
30	24.28184001784148	25.607398711857517	26.069100313790646	24.04166095651036
31	24.239041787339392	25.099657631350937	26.366942639882208	24.294357941427467
32	23.722385125267788	25.60840461643139	26.301997313804915	24.367212944495904
33	23.814902553639218	25.155652985963243	26.356823411818535	24.672621048579003
34	24.16262742962494	24.930562493240235	26.723961386332522	24.1828486908023
35	23.915321061526797	24.977711046087453	26.679538233057258	24.427429659328492
36	23.732444171006527	24.639108091066518	27.0002670375732	24.628180700353752
37	23.540514139321395	24.190715380418496	27.483986772956676	24.784783707303433
38	23.347122536887895	24.136731834953927	27.861364340184203	24.65478128797397
39	23.01167485518843	24.07052268005305	28.38393606504918	24.533866399709336
40	23.163841565043082	23.91288797610452	28.511342046931553	24.411928411920847
41	22.605667696242953	24.08335871021368	28.659020874841225	24.651952718702137
42	22.259774082430184	24.37148588956612	28.56005534022702	24.808684687776676
43	22.105303249278112	24.11633862427676	28.541949057897288	25.23640906854784
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6594.0
1	5667.0
2	4740.0
3	8297.5
4	11855.0
5	11855.0
6	13808.0
7	15761.0
8	14306.0
9	12851.0
10	17892.0
11	22933.0
12	22933.0
13	35545.0
14	48157.0
15	57741.0
16	67325.0
17	64543.5
18	61762.0
19	61762.0
20	73281.0
21	84800.0
22	80733.0
23	76666.0
24	92014.0
25	107362.0
26	107362.0
27	131474.0
28	155586.0
29	179790.5
30	203995.0
31	238781.5
32	273568.0
33	273568.0
34	315348.0
35	357128.0
36	406618.5
37	456109.0
38	510156.0
39	564203.0
40	564203.0
41	620165.5
42	676128.0
43	734703.5
44	793279.0
45	846774.0
46	900269.0
47	900269.0
48	946671.0
49	993073.0
50	1010947.5
51	1028822.0
52	1014612.0
53	1000402.0
54	1000402.0
55	955632.5
56	910863.0
57	845571.0
58	780279.0
59	710613.0
60	640947.0
61	640947.0
62	560035.0
63	479123.0
64	406123.0
65	333123.0
66	276402.0
67	219681.0
68	219681.0
69	178677.5
70	137674.0
71	110853.0
72	84032.0
73	68377.0
74	52722.0
75	52722.0
76	42346.5
77	31971.0
78	25321.0
79	18671.0
80	14255.5
81	9840.0
82	9840.0
83	7441.0
84	5042.0
85	3715.5
86	2389.0
87	1746.0
88	1103.0
89	1103.0
90	717.5
91	332.0
92	224.5
93	117.0
94	76.0
95	35.0
96	35.0
97	21.0
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.1631322E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.304362901897164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.11346490339811	38.020302582198504
2	11.238452884724396	11.082095189686493
3	4.065411505104718	6.0132757257969285
4	2.0853179260312746	4.112610871635101
5	1.223824636947414	3.016994701416892
6	0.8126781715110727	2.4041147694379297
7	0.5689173550766355	1.9635075415120145
8	0.4098891075039018	1.616745704472569
9	0.313684981646873	1.3919434354793137
>10	1.9449637890437441	18.070504139906387
>50	0.15392946169806904	5.215870221961021
>100	0.06787171595092169	5.523746305286005
>500	0.0012618378206453438	0.3966468707713935
>1k	2.0951065598030858E-4	0.21082965296000214
>5k	5.237695124106411E-5	0.14495079744184525
>10k+	6.983593498808547E-5	0.8158614900374518
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30335	0.26080440383302944	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27288	0.23460789753735645	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21474	0.18462217794331545	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15682	0.13482560279906272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.597474990375128E-6
2	0.0	8.597474990375128E-6	0.0	0.0	8.597474990375128E-6
3	0.0	8.597474990375128E-6	0.0	0.0	8.597474990375128E-6
4	0.0	8.597474990375128E-6	0.0	0.0	1.7194949980750255E-5
5	0.0	8.597474990375128E-6	0.0	8.597474990375128E-6	1.7194949980750255E-5
6	1.7194949980750255E-5	8.597474990375128E-6	0.0	8.597474990375128E-6	5.158484994225076E-5
7	1.7194949980750255E-5	8.597474990375128E-6	0.0	8.597474990375128E-6	6.0182324932625884E-5
8	2.579242497112538E-5	8.597474990375128E-6	0.0	8.597474990375128E-6	7.737727491337614E-5
9	2.579242497112538E-5	8.597474990375128E-6	0.0	1.7194949980750255E-5	1.1176717487487665E-4
10	3.438989996150051E-5	8.597474990375128E-6	0.0	2.579242497112538E-5	1.1176717487487665E-4
11	3.438989996150051E-5	8.597474990375128E-6	0.0	3.438989996150051E-5	1.1176717487487665E-4
12	3.438989996150051E-5	8.597474990375128E-6	0.0	3.438989996150051E-5	1.1176717487487665E-4
13	3.438989996150051E-5	8.597474990375128E-6	0.0	3.438989996150051E-5	1.1176717487487665E-4
14	3.438989996150051E-5	8.597474990375128E-6	0.0	6.0182324932625884E-5	1.4615707483637714E-4
15	3.438989996150051E-5	8.597474990375128E-6	0.0	6.877979992300102E-5	2.235343497497533E-4
16	4.298737495187563E-5	8.597474990375128E-6	0.0	1.1176717487487665E-4	2.4932677472087867E-4
17	4.298737495187563E-5	8.597474990375128E-6	0.0	1.289621248556269E-4	2.579242497112538E-4
18	4.298737495187563E-5	8.597474990375128E-6	0.0	1.633520248171274E-4	3.0091162466312944E-4
19	5.158484994225076E-5	8.597474990375128E-6	0.0	2.235343497497533E-4	3.0091162466312944E-4
20	5.158484994225076E-5	1.7194949980750255E-5	0.0	3.267040496342548E-4	3.0091162466312944E-4
21	6.0182324932625884E-5	1.7194949980750255E-5	0.0	5.158484994225076E-4	3.0091162466312944E-4
22	6.0182324932625884E-5	1.7194949980750255E-5	0.0	9.027348739893883E-4	3.0091162466312944E-4
23	6.0182324932625884E-5	1.7194949980750255E-5	0.0	0.0013927909484407705	3.9548384955725584E-4
24	6.877979992300102E-5	1.7194949980750255E-5	0.0	0.002441682897266536	4.126787995380061E-4
25	9.45722248941264E-5	1.7194949980750255E-5	0.0	0.0036367319209286786	4.3847122450913146E-4
26	9.45722248941264E-5	1.7194949980750255E-5	0.0	0.007393828491722609	4.3847122450913146E-4
27	9.45722248941264E-5	1.7194949980750255E-5	0.0	0.03319485093783837	4.3847122450913146E-4
28	1.0316969988450152E-4	1.7194949980750255E-5	0.0	0.10126106043663824	4.3847122450913146E-4
29	1.0316969988450152E-4	1.7194949980750255E-5	0.0	0.18964310333769455	4.3847122450913146E-4
30	1.0316969988450152E-4	1.7194949980750255E-5	0.0	0.3001722418139572	4.3847122450913146E-4
31	1.0316969988450152E-4	1.7194949980750255E-5	0.0	0.6396693342338902	4.3847122450913146E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11055	0.0	20.616915	1
CTTATAC	9500	0.0	16.12421	37
GTATCAA	15510	0.0	14.635397	2
TCTTATA	14975	0.0	14.268782	37
CTCTTAT	22880	0.0	12.605551	37
GTATAGA	1420	0.0	11.725352	1
TCGTTAC	1035	0.0	11.26087	23
TATCAAC	21050	0.0	10.950594	3
CTCGTTA	1185	0.0	10.928269	22
AACGCAG	20735	0.0	10.876055	7
ATCAACG	20940	0.0	10.840258	4
TCAACGC	21235	0.0	10.654816	5
TCTCTTA	32165	0.0	10.6462	37
CAACGCA	21335	0.0	10.570189	6
GTACCGT	720	0.0	10.534722	6
TTAGACA	2045	0.0	10.403422	4
TATACAC	4000	0.0	10.36	37
GGGTAAG	3395	0.0	10.189985	1
TATACTG	2045	0.0	10.041565	5
GTATTAC	1080	0.0	9.935185	1
>>END_MODULE