##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2727139.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12879629 Sequences flagged as poor quality 0 Sequence length 43 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.951873924318782 33.0 31.0 34.0 30.0 34.0 2 32.18639403355485 33.0 31.0 34.0 30.0 34.0 3 32.30640447795507 34.0 31.0 34.0 30.0 34.0 4 35.91894525843873 37.0 35.0 37.0 35.0 37.0 5 35.624258043457615 37.0 35.0 37.0 33.0 37.0 6 35.63302491088835 37.0 35.0 37.0 33.0 37.0 7 35.599775583597946 37.0 35.0 37.0 33.0 37.0 8 35.56436307288044 37.0 35.0 37.0 33.0 37.0 9 37.38491364929844 39.0 37.0 39.0 34.0 39.0 10 37.20175091999933 39.0 37.0 39.0 33.0 39.0 11 37.292032868338055 39.0 37.0 39.0 34.0 39.0 12 37.19458161411326 39.0 37.0 39.0 33.0 39.0 13 37.26695963059184 39.0 37.0 39.0 34.0 39.0 14 38.463847988167984 40.0 38.0 41.0 34.0 41.0 15 38.417966620001245 40.0 38.0 41.0 34.0 41.0 16 38.380714227094586 40.0 38.0 41.0 34.0 41.0 17 38.32585728983343 40.0 38.0 41.0 34.0 41.0 18 38.32306932132905 40.0 38.0 41.0 34.0 41.0 19 38.37536345185098 40.0 38.0 41.0 34.0 41.0 20 38.29101040099835 40.0 38.0 41.0 34.0 41.0 21 38.24061337481072 40.0 38.0 41.0 34.0 41.0 22 38.1818011993979 40.0 38.0 41.0 34.0 41.0 23 38.129221967496115 40.0 38.0 41.0 33.0 41.0 24 38.05172291841636 40.0 37.0 41.0 33.0 41.0 25 38.023362629467044 40.0 37.0 41.0 33.0 41.0 26 37.87483987310504 40.0 37.0 41.0 33.0 41.0 27 37.75173896701528 40.0 37.0 41.0 33.0 41.0 28 37.679781381901606 40.0 37.0 41.0 32.0 41.0 29 37.603203089157304 40.0 37.0 41.0 32.0 41.0 30 37.561554839817205 39.0 37.0 41.0 32.0 41.0 31 37.532100031763335 39.0 37.0 41.0 32.0 41.0 32 37.440989332844914 39.0 37.0 41.0 32.0 41.0 33 37.3822159007841 39.0 37.0 41.0 32.0 41.0 34 37.35478257952927 39.0 36.0 41.0 31.0 41.0 35 37.295524583821475 39.0 36.0 41.0 31.0 41.0 36 37.19577403976466 39.0 36.0 41.0 31.0 41.0 37 37.130551974750205 39.0 36.0 41.0 31.0 41.0 38 37.10843643089409 39.0 36.0 41.0 31.0 41.0 39 37.02736142477396 39.0 36.0 40.0 31.0 41.0 40 36.96243067249841 39.0 36.0 40.0 31.0 41.0 41 36.93054598078873 39.0 36.0 40.0 31.0 41.0 42 36.82761234815071 39.0 36.0 40.0 30.0 41.0 43 35.54381092809428 38.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 3.0 9 5.0 10 8.0 11 10.0 12 6.0 13 7.0 14 6.0 15 13.0 16 50.0 17 86.0 18 236.0 19 586.0 20 1270.0 21 2765.0 22 5369.0 23 10287.0 24 18077.0 25 29613.0 26 46643.0 27 70712.0 28 104283.0 29 148079.0 30 204456.0 31 276354.0 32 362010.0 33 476717.0 34 637488.0 35 869361.0 36 1231858.0 37 1880301.0 38 3342684.0 39 3160284.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40556929085457 18.493180199522826 12.465599746700779 26.63565076292182 2 18.13554567449109 20.3869614567314 36.18886071951296 25.28863214926455 3 19.584508218365606 22.40691870860566 29.920496933568508 28.088076139460227 4 14.770371103080688 15.538491054361891 34.492026129013496 35.19911171354392 5 15.328733459636142 35.581234521584435 34.119414464500494 14.970617554278931 6 35.261481522487955 34.440572783579405 15.434489611463187 14.863456082469456 7 29.298638959243313 30.114050645402905 21.539665467072073 19.047644928281706 8 26.07973412898772 35.303516894780124 19.767564733425164 18.84918424280699 9 26.62868627660005 14.977356878835563 19.645775511080327 38.74818133348406 10 16.936217650368658 26.439659092664858 33.51148546281885 23.11263779414764 11 35.716028776915856 21.76599962623147 21.704677984125166 20.81329361272751 12 21.308719373826683 24.595599764558436 30.728897548213542 23.366783313401342 13 30.59817949725105 19.942499896542053 24.40769838944895 25.051622216757952 14 22.22379231575692 20.402179286375407 25.674792340679996 31.699236057187676 15 24.891128463405273 27.571881146576505 22.617413902217216 24.919576487801006 16 24.519277690374466 26.222579858472635 24.63734786149508 24.620794589657823 17 23.94045666998638 26.21438862874078 25.60433223658849 24.240822464684346 18 24.108745678932213 25.614487808616225 26.184131546025124 24.09263496642644 19 24.93512041379453 25.359169895344035 26.308459661376894 23.397250029484546 20 24.42667409131117 25.204398356505454 26.294088129401867 24.074839422781512 21 24.186496365695007 25.402074857901574 26.54223968718354 23.869189089219883 22 24.674732478707266 25.02887311427992 25.955413777834753 24.34098062917806 23 24.256576024045412 25.24988103306392 26.034181574640076 24.459361368250594 24 24.162598161794875 25.303143436817937 26.062303502686294 24.471954898700886 25 24.56895303428383 25.043531921610473 25.914822546519 24.472692497586692 26 24.201473505176274 25.368090959762895 26.083057206073253 24.34737832898758 27 24.44812657258994 25.662183281832107 25.716082349887564 24.173607795690387 28 24.2611957223302 25.510051570584835 25.77659651531888 24.452156191766083 29 24.18856940677406 25.75356790168413 26.09650479839132 23.961357893150495 30 24.491062591942672 25.488397220137323 25.843221105204194 24.177319082715815 31 24.36670341979571 25.015937959082517 26.225693302190617 24.391665318931157 32 23.860912453301257 25.53766106151039 26.15547388826184 24.44595259692651 33 23.942032802342368 25.070326171662245 26.22020401364045 24.767437012354936 34 24.352588106381017 24.869101431415455 26.62777010114189 24.150540361061644 35 24.101587087640493 24.91338842135903 26.52055427994083 24.46447021105965 36 23.91547147825454 24.62839574028103 26.81237945596104 24.643753325503397 37 23.709502812542194 24.186278968128665 27.215248203189702 24.88897001613944 38 23.58644802579329 24.0920293589202 27.53653851364818 24.784984101638333 39 23.279086687978356 24.085445318339527 28.06124306841447 24.574224925267647 40 23.43074478309895 23.896588946777893 28.20748951697289 24.465176753150267 41 22.875255180098744 24.027648622487497 28.38143862684244 24.715657570571324 42 22.498349913650465 24.30657746430429 28.3533865765854 24.84168604545985 43 22.341443220142445 23.984596140152796 28.285721584061157 25.38823905564361 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6270.0 1 5478.5 2 4687.0 3 8303.5 4 11920.0 5 11920.0 6 13745.0 7 15570.0 8 13881.5 9 12193.0 10 16665.5 11 21138.0 12 21138.0 13 32698.5 14 44259.0 15 51514.5 16 58770.0 17 58625.5 18 58481.0 19 58481.0 20 70742.0 21 83003.0 22 83357.5 23 83712.0 24 101037.5 25 118363.0 26 118363.0 27 144659.0 28 170955.0 29 198082.0 30 225209.0 31 263962.5 32 302716.0 33 302716.0 34 349697.0 35 396678.0 36 450061.5 37 503445.0 38 562960.0 39 622475.0 40 622475.0 41 684436.0 42 746397.0 43 811499.5 44 876602.0 45 937943.0 46 999284.0 47 999284.0 48 1053258.5 49 1107233.0 50 1131551.0 51 1155869.0 52 1140707.0 53 1125545.0 54 1125545.0 55 1072739.0 56 1019933.0 57 946708.5 58 873484.0 59 794367.0 60 715250.0 61 715250.0 62 624971.0 63 534692.0 64 453483.5 65 372275.0 66 307318.0 67 242361.0 68 242361.0 69 196285.5 70 150210.0 71 120661.0 72 91112.0 73 73946.5 74 56781.0 75 56781.0 76 45420.5 77 34060.0 78 26894.0 79 19728.0 80 14916.0 81 10104.0 82 10104.0 83 7512.0 84 4920.0 85 3648.0 86 2376.0 87 1698.0 88 1020.0 89 1020.0 90 700.5 91 381.0 92 239.5 93 98.0 94 73.0 95 48.0 96 48.0 97 30.5 98 13.0 99 11.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 1.2879629E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.25877026228169 #Duplication Level Percentage of deduplicated Percentage of total 1 76.80428861528627 37.064805194430775 2 11.135125726640709 10.74734948567153 3 4.162977178480523 6.027004777902397 4 2.084770436191645 4.024338301190776 5 1.278123846233356 3.0840342531059686 6 0.8522758768459288 2.4677871444475414 7 0.6051543624802772 2.044280374650727 8 0.4329233569717988 1.6713879060222245 9 0.3358455970835004 1.4586745961926322 >10 2.0714544835451663 18.652107088361873 >50 0.1638728981630233 5.420058703085192 >100 0.07093713779583587 5.735147524280321 >500 0.0018476098811682036 0.5958148853585966 >1k 3.061872031521385E-4 0.2800283994678637 >5k 3.222906586625341E-5 0.0848901889615396 >10k+ 6.445813173250682E-5 0.6422911768698679 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 26047 0.20223408609052326 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 22766 0.17675974983440904 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 19360 0.15031488872854956 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14419 0.11195198246781797 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.211359038369817E-5 2 0.0 0.0 0.0 0.0 7.764198797962271E-5 3 0.0 0.0 0.0 0.0 7.764198797962271E-5 4 0.0 0.0 0.0 1.552839759592454E-5 1.0093458437350952E-4 5 0.0 7.76419879796227E-6 0.0 1.552839759592454E-5 1.0093458437350952E-4 6 0.0 7.76419879796227E-6 0.0 1.552839759592454E-5 1.5528397595924542E-4 7 0.0 7.76419879796227E-6 0.0 1.552839759592454E-5 1.6304817475720768E-4 8 0.0 7.76419879796227E-6 0.0 1.552839759592454E-5 1.8634077115109449E-4 9 0.0 7.76419879796227E-6 0.0 1.552839759592454E-5 2.717469579286795E-4 10 0.0 7.76419879796227E-6 0.0 2.329259639388681E-5 2.717469579286795E-4 11 0.0 7.76419879796227E-6 0.0 2.329259639388681E-5 2.717469579286795E-4 12 0.0 7.76419879796227E-6 0.0 3.105679519184908E-5 2.717469579286795E-4 13 0.0 7.76419879796227E-6 0.0 3.105679519184908E-5 2.7951115672664173E-4 14 0.0 7.76419879796227E-6 0.0 4.658519278777362E-5 3.416247471103399E-4 15 0.0 1.552839759592454E-5 0.0 5.4349391585735894E-5 3.5715314470626447E-4 16 7.76419879796227E-6 1.552839759592454E-5 0.0 8.540618677758498E-5 4.192667350899626E-4 17 7.76419879796227E-6 1.552839759592454E-5 0.0 1.1646298196943405E-4 4.270309338879249E-4 18 7.76419879796227E-6 1.552839759592454E-5 0.0 1.3975557836332086E-4 4.736161266756985E-4 19 7.76419879796227E-6 1.552839759592454E-5 0.0 1.8634077115109449E-4 4.736161266756985E-4 20 7.76419879796227E-6 1.552839759592454E-5 0.0 2.717469579286795E-4 4.736161266756985E-4 21 1.552839759592454E-5 1.552839759592454E-5 0.0 4.3479513268588715E-4 4.969087230695853E-4 22 1.552839759592454E-5 1.552839759592454E-5 0.0 6.987778918166043E-4 4.969087230695853E-4 23 1.552839759592454E-5 1.552839759592454E-5 0.0 0.001118044626906567 6.444285002308685E-4 24 1.552839759592454E-5 1.552839759592454E-5 0.0 0.0017702373259353976 6.521926990288307E-4 25 1.552839759592454E-5 3.105679519184908E-5 0.0 0.0029426313444277006 6.910136930186421E-4 26 1.552839759592454E-5 3.105679519184908E-5 0.0 0.005784328104481892 7.298346870084534E-4 27 2.329259639388681E-5 3.105679519184908E-5 0.0 0.02793558727506825 7.298346870084534E-4 28 2.329259639388681E-5 3.105679519184908E-5 0.0 0.0904839727914523 7.298346870084534E-4 29 2.329259639388681E-5 3.105679519184908E-5 0.0 0.17109964891069457 7.298346870084534E-4 30 3.105679519184908E-5 3.105679519184908E-5 0.0 0.2699767206027441 7.298346870084534E-4 31 3.105679519184908E-5 3.105679519184908E-5 0.0 0.5945357587551628 7.298346870084534E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7195 0.0 25.37804 1 CTTATAC 9315 0.0 16.424585 37 GTATCAA 11325 0.0 16.15585 2 TCTTATA 15445 0.0 14.529297 37 CTCTTAT 23200 0.0 12.080819 37 TATACTG 2105 0.0 11.688837 5 TATCAAC 16065 0.0 11.584812 3 ATCAACG 16100 0.0 11.375777 4 TCAACGC 16160 0.0 11.322092 5 CAACGCA 16430 0.0 11.147291 6 TCGTTAC 1380 0.0 11.126812 23 GGGTAAG 4995 0.0 10.777778 1 AACGCAG 17325 0.0 10.635497 7 TTATACT 2250 0.0 10.606667 4 CTCGTTA 1540 0.0 10.4513 22 TAGGACT 2105 0.0 10.370546 4 TCTCTTA 32245 0.0 10.287021 37 GTATAGA 1605 0.0 10.143303 1 TAGACAG 2560 0.0 10.1171875 5 GTAATAC 1135 0.0 10.105727 3 >>END_MODULE