FastQCFastQC Report
Thu 9 Feb 2017
SRR2727138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590195
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144940.40371066195568767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT134020.3732944867897148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96360.268397677563475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73280.2041114758390561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40900.026.6418111
GTATCAA62400.017.4623372
CTTATAC23000.017.21304337
CGTATAC750.00410666414.83
TCTTATA38300.013.71801537
CGTCTAG1307.00588E-512.8076931
ATACCGT1602.6993348E-612.718756
TCGTTAC7650.012.57516323
ACTATAC3250.012.5230773
CTCGTTA8050.012.40993822
CGCTACG2102.8967406E-812.33333410
TACTTAC13950.012.20071731
CTCTTAT56500.011.98407137
TATCAAC91450.011.8950243
CTAACCT5300.011.8679254
ATCAACG94550.011.7202544
GTACTTA14500.011.61034530
TCAACGC96200.011.4807695
ATTATAC5000.011.4699993
CAACGCA96450.011.4318296
Produced by FastQC (version 0.11.5)