##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727138.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3590195
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.53252065695596	31.0	30.0	31.0	27.0	34.0
2	30.754715830198638	31.0	31.0	33.0	27.0	34.0
3	30.824679160881235	31.0	31.0	34.0	27.0	34.0
4	34.65383495882536	35.0	35.0	37.0	32.0	37.0
5	33.498749510820446	35.0	33.0	37.0	28.0	37.0
6	33.56771790947288	35.0	33.0	37.0	28.0	37.0
7	33.56717476348778	35.0	33.0	37.0	28.0	37.0
8	33.46736207921854	35.0	33.0	37.0	28.0	37.0
9	35.02670551321029	37.0	34.0	39.0	29.0	39.0
10	34.573113716664416	37.0	33.0	39.0	28.0	39.0
11	34.8446925584822	37.0	33.0	39.0	29.0	39.0
12	34.61910564746483	37.0	33.0	39.0	27.0	39.0
13	34.83086740413822	37.0	33.0	39.0	29.0	39.0
14	35.555389609756574	38.0	34.0	40.0	28.0	40.0
15	35.47987142759655	38.0	33.0	39.0	27.0	40.0
16	35.37875797832708	38.0	33.0	39.0	27.0	40.0
17	35.31606973994449	37.0	33.0	39.0	27.0	40.0
18	35.27768714512722	37.0	33.0	39.0	27.0	40.0
19	35.33757859949112	38.0	34.0	40.0	27.0	40.0
20	35.205167407341385	37.0	33.0	39.0	27.0	40.0
21	35.09853503779043	37.0	33.0	39.0	27.0	40.0
22	35.020166314086005	37.0	33.0	39.0	27.0	40.0
23	34.905020479389	37.0	33.0	39.0	27.0	40.0
24	34.749591874536065	37.0	33.0	39.0	26.0	40.0
25	34.70554496343513	37.0	33.0	39.0	26.0	40.0
26	34.39732103687961	37.0	32.0	39.0	25.0	40.0
27	34.19060329592125	37.0	32.0	39.0	25.0	40.0
28	34.042132251869326	37.0	32.0	39.0	24.0	40.0
29	33.87209413416263	37.0	32.0	39.0	24.0	40.0
30	33.77982282299429	36.0	31.0	39.0	24.0	40.0
31	33.681477747030456	36.0	31.0	39.0	24.0	40.0
32	33.5236548432606	36.0	31.0	39.0	24.0	40.0
33	33.404927030425924	36.0	31.0	39.0	23.0	40.0
34	33.33301951565305	36.0	31.0	39.0	23.0	40.0
35	33.21221187150002	36.0	31.0	39.0	23.0	40.0
36	33.010573520379815	36.0	30.0	38.0	21.0	40.0
37	32.91939184361852	36.0	30.0	38.0	21.0	40.0
38	32.849900632138365	36.0	30.0	38.0	21.0	40.0
39	32.685601757007625	35.0	30.0	38.0	20.0	40.0
40	32.58015762375024	35.0	30.0	38.0	19.0	40.0
41	32.49324730272311	35.0	30.0	38.0	19.0	40.0
42	32.30108225319238	35.0	30.0	38.0	18.0	40.0
43	31.03594205885753	34.0	27.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	9.0
11	7.0
12	12.0
13	27.0
14	59.0
15	144.0
16	329.0
17	676.0
18	1512.0
19	2967.0
20	5406.0
21	9369.0
22	15118.0
23	23423.0
24	35070.0
25	49888.0
26	68760.0
27	91109.0
28	117641.0
29	149128.0
30	184014.0
31	223842.0
32	269371.0
33	322228.0
34	379059.0
35	434212.0
36	454676.0
37	403093.0
38	272514.0
39	76525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.51610010041237	20.701772466398065	13.842200771824373	24.93992666136519
2	19.241294692906653	22.549332278608823	34.8512545975915	23.35811843089303
3	20.624868565635015	23.91624967446058	30.43219100912346	25.026690750780944
4	16.332873284041675	17.00604006189079	33.92172291477204	32.7393637392955
5	15.035673549765402	36.74973086420097	33.59296639876107	14.621629187272559
6	34.20477160711326	35.32666052958126	15.280562754947852	15.188005108357624
7	28.154849527671892	31.489877290787827	21.586459788395896	18.768813393144384
8	25.867926393970244	36.05444829598392	19.773354929189082	18.30427038085675
9	26.922186677882397	15.108037307165766	19.81669519343657	38.15308082151527
10	16.829058031666804	27.663984825336783	33.252065695595924	22.25489144740049
11	35.36994508654822	22.46852886820911	21.522563537635143	20.638962507607523
12	20.970866484968088	25.454912616167086	30.6319294634414	22.942291435423424
13	30.488594630653765	20.79017992058927	24.59571137500888	24.125514073748082
14	21.844746594544308	21.42223472541185	26.352691149088002	30.380327530955842
15	24.929453692626723	28.29578894739701	23.14169564605822	23.633061713918046
16	23.555433618508186	27.061148489148916	25.570477369613627	23.812940522729267
17	23.511842671498346	26.827762837394626	25.942574149872083	23.717820341234948
18	23.410650396426934	26.264033012134437	26.850268578726226	23.475048012712403
19	24.758515902339568	25.8243354469604	27.22428726016275	22.19286139053728
20	23.91140314105501	25.57554673214129	27.18047348403081	23.332576642772885
21	23.62587547473048	25.98741851069371	27.741947164429785	22.644758850146022
22	24.1826418899252	25.490927373025702	26.69164209743482	23.634788639614282
23	23.55401308285483	25.877396631659284	27.09426646742029	23.474323818065592
24	23.328760694057006	25.75255661600554	27.1589983273889	23.75968436254855
25	23.905414608398708	25.50903223919592	26.891491966313808	23.694061186091563
26	23.495548291945145	25.820213108201646	27.071927847930265	23.612310751922948
27	23.997944401348672	26.276400028410713	26.541622390984333	23.184033179256282
28	23.513625304475106	26.49296208144683	26.297903038692883	23.69550957538518
29	23.580167651060734	26.525829376955844	26.988617609906985	22.905385362076434
30	24.292970159002504	26.024101754918604	26.413356377578378	23.269571708500514
31	23.868619949612764	25.67409291138782	27.127997225777428	23.329289913221984
32	23.3763347116243	26.22049777240512	26.84160609660478	23.561561419365802
33	23.46983938198343	25.415304739714696	26.93048706268044	24.184368815621436
34	23.777454985035632	25.635599180545903	27.50923000004178	23.077715834376683
35	23.71667834198421	25.468226656212266	27.173287244843248	23.641807756960276
36	23.481788593655775	25.250745433047513	27.481264945218854	23.786201028077862
37	23.05540506852692	25.15398188677774	28.02984239017658	23.760770654518765
38	23.49150951410717	24.69539955350615	27.9430225934803	23.870068338906382
39	22.671414783876642	24.977027710194015	28.7087748715599	23.642782634369443
40	23.362157208731002	24.55114555058987	28.62267926951043	23.464017971168698
41	22.60058297669068	24.791578173330418	28.685294252819137	23.922544597159764
42	22.274528263785115	25.13974867660392	28.58633026896868	23.99939279064229
43	22.10910549426981	24.766927701698656	28.300022700716816	24.823944103314723
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5097.0
1	4112.5
2	3128.0
3	4194.0
4	5260.0
5	5260.0
6	6306.0
7	7352.0
8	6909.5
9	6467.0
10	8662.0
11	10857.0
12	10857.0
13	16873.5
14	22890.0
15	27733.5
16	32577.0
17	30615.5
18	28654.0
19	28654.0
20	33465.5
21	38277.0
22	34818.0
23	31359.0
24	36268.5
25	41178.0
26	41178.0
27	48603.0
28	56028.0
29	63423.5
30	70819.0
31	80839.0
32	90859.0
33	90859.0
34	102768.5
35	114678.0
36	127937.5
37	141197.0
38	156304.5
39	171412.0
40	171412.0
41	188106.0
42	204800.0
43	221806.5
44	238813.0
45	256911.5
46	275010.0
47	275010.0
48	292674.5
49	310339.0
50	316527.5
51	322716.0
52	315568.5
53	308421.0
54	308421.0
55	288195.5
56	267970.0
57	246595.0
58	225220.0
59	204367.5
60	183515.0
61	183515.0
62	158883.0
63	134251.0
64	112979.0
65	91707.0
66	75023.5
67	58340.0
68	58340.0
69	47196.5
70	36053.0
71	29107.0
72	22161.0
73	18019.0
74	13877.0
75	13877.0
76	11108.0
77	8339.0
78	6633.0
79	4927.0
80	3802.0
81	2677.0
82	2677.0
83	2099.0
84	1521.0
85	1182.0
86	843.0
87	630.5
88	418.0
89	418.0
90	279.0
91	140.0
92	85.0
93	30.0
94	21.5
95	13.0
96	13.0
97	8.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3590195.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.93507265574583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.15047167824822	55.29252065098076
2	7.851726264956471	10.197048309759527
3	2.6004467034793017	5.065805868834696
4	1.2681095554421211	3.293791444723187
5	0.7564401964969921	2.4559749559629416
6	0.4946771374747743	1.9273137517836503
7	0.3292989376390134	1.4968135308734476
8	0.24814953269585516	1.2890886348075785
9	0.19181820512219194	1.121015617767386
>10	1.043138964878984	11.96843279701595
>50	0.04746638636385615	2.061581314605884
>100	0.017397517783911444	1.9542086197874287
>500	3.435677688694121E-4	0.16503525112546394
>1k	3.4356776622071293E-4	0.4603585518764739
>5k	8.589194155517804E-5	0.47307502265765056
>10k+	8.589194155517804E-5	0.7779356774379756
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14494	0.40371066195568767	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13402	0.3732944867897148	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9636	0.268397677563475	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7328	0.2041114758390561	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.7853640261879927E-5	0.0	0.0	0.0	0.0
9	2.7853640261879927E-5	0.0	0.0	0.0	2.7853640261879927E-5
10	2.7853640261879927E-5	0.0	0.0	0.0	2.7853640261879927E-5
11	2.7853640261879927E-5	0.0	0.0	2.7853640261879927E-5	2.7853640261879927E-5
12	2.7853640261879927E-5	0.0	0.0	2.7853640261879927E-5	2.7853640261879927E-5
13	2.7853640261879927E-5	0.0	0.0	2.7853640261879927E-5	2.7853640261879927E-5
14	5.5707280523759855E-5	0.0	0.0	8.356092078563977E-5	2.7853640261879927E-5
15	8.356092078563977E-5	0.0	0.0	1.1141456104751971E-4	5.5707280523759855E-5
16	8.356092078563977E-5	0.0	0.0	1.9497548183315948E-4	8.356092078563977E-5
17	8.356092078563977E-5	0.0	0.0	1.9497548183315948E-4	8.356092078563977E-5
18	8.356092078563977E-5	0.0	0.0	2.5068276235691936E-4	8.356092078563977E-5
19	8.356092078563977E-5	0.0	0.0	3.342436831425591E-4	8.356092078563977E-5
20	8.356092078563977E-5	0.0	0.0	4.7351188445195875E-4	8.356092078563977E-5
21	8.356092078563977E-5	0.0	0.0	7.241946468088781E-4	8.356092078563977E-5
22	8.356092078563977E-5	0.0	0.0	9.748774091657974E-4	8.356092078563977E-5
23	8.356092078563977E-5	0.0	0.0	0.0012534138117845967	1.3926820130939963E-4
24	8.356092078563977E-5	0.0	0.0	0.002172583940426634	1.3926820130939963E-4
25	8.356092078563977E-5	0.0	0.0	0.0030917540690686717	1.3926820130939963E-4
26	8.356092078563977E-5	0.0	0.0	0.005375752570542826	1.6712184157127954E-4
27	8.356092078563977E-5	0.0	0.0	0.02242218041081334	1.6712184157127954E-4
28	8.356092078563977E-5	0.0	0.0	0.0718623918756502	1.6712184157127954E-4
29	8.356092078563977E-5	0.0	0.0	0.14185858985375446	1.6712184157127954E-4
30	8.356092078563977E-5	0.0	0.0	0.23021033676443758	1.6712184157127954E-4
31	8.356092078563977E-5	0.0	0.0	0.5272415565171251	1.6712184157127954E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4090	0.0	26.641811	1
GTATCAA	6240	0.0	17.462337	2
CTTATAC	2300	0.0	17.213043	37
CGTATAC	75	0.004106664	14.8	3
TCTTATA	3830	0.0	13.718015	37
CGTCTAG	130	7.00588E-5	12.807693	1
ATACCGT	160	2.6993348E-6	12.71875	6
TCGTTAC	765	0.0	12.575163	23
ACTATAC	325	0.0	12.523077	3
CTCGTTA	805	0.0	12.409938	22
CGCTACG	210	2.8967406E-8	12.333334	10
TACTTAC	1395	0.0	12.200717	31
CTCTTAT	5650	0.0	11.984071	37
TATCAAC	9145	0.0	11.895024	3
CTAACCT	530	0.0	11.867925	4
ATCAACG	9455	0.0	11.720254	4
GTACTTA	1450	0.0	11.610345	30
TCAACGC	9620	0.0	11.480769	5
ATTATAC	500	0.0	11.469999	3
CAACGCA	9645	0.0	11.431829	6
>>END_MODULE