FastQCFastQC Report
Thu 9 Feb 2017
SRR2727137.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727137.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6315772
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT217190.34388511808216005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT210240.332880920970548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT162610.257466545657443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111200.17606715378579216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC49100.017.14358537
GGTATCA100100.015.9310681
TCTTATA78750.013.0850837
TAACACG1755.316015E-712.6857144
CTCTTAT115550.012.32799737
TCGATTA3401.8189894E-1211.42647113
GTATCAA141750.011.2761912
TCGTTAC6600.011.21212123
TATACTG9300.011.1397855
TTATACT11300.010.8053094
CTATACT7550.010.7814574
TAATCGA3950.010.77215220
TCTCTTA165500.010.73111837
GTATAAC6400.010.69531251
GTCTAGG7450.010.6778521
GGGTATA6950.010.6474821
CTCGTTA8750.010.57142822
TAGATCG2801.2687451E-810.5714285
CTAGAGT10200.010.5196084
TAGGACT9000.010.4833344
Produced by FastQC (version 0.11.5)