##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727137.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6315772
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.411843397766734	31.0	31.0	34.0	30.0	34.0
2	31.6629294091047	31.0	31.0	34.0	30.0	34.0
3	31.775258036547235	31.0	31.0	34.0	30.0	34.0
4	35.534567428969886	37.0	35.0	37.0	33.0	37.0
5	35.08604664639572	37.0	35.0	37.0	32.0	37.0
6	35.09782367064549	37.0	35.0	37.0	32.0	37.0
7	35.04354527047525	36.0	35.0	37.0	32.0	37.0
8	34.99069979093609	36.0	35.0	37.0	32.0	37.0
9	36.80493580198905	39.0	37.0	39.0	33.0	39.0
10	36.544968691080044	38.0	35.0	39.0	32.0	39.0
11	36.660882311774394	38.0	35.0	39.0	32.0	39.0
12	36.52906849708951	38.0	35.0	39.0	32.0	39.0
13	36.684339618339614	38.0	35.0	39.0	32.0	39.0
14	37.687417785189204	39.0	37.0	40.0	33.0	41.0
15	37.68290875604756	39.0	37.0	40.0	32.0	41.0
16	37.616909698450165	39.0	37.0	40.0	32.0	41.0
17	37.5398752519882	39.0	36.0	40.0	32.0	41.0
18	37.544697623663424	39.0	36.0	40.0	32.0	41.0
19	37.56704548549251	39.0	37.0	40.0	32.0	41.0
20	37.4430804341892	39.0	36.0	40.0	32.0	41.0
21	37.45030615418036	39.0	36.0	40.0	32.0	41.0
22	37.35698707933092	39.0	36.0	40.0	32.0	41.0
23	37.28740714515977	39.0	36.0	40.0	32.0	41.0
24	37.16406877892362	39.0	36.0	40.0	31.0	41.0
25	37.13486443145826	39.0	36.0	40.0	31.0	41.0
26	36.98860076012877	39.0	36.0	40.0	31.0	41.0
27	36.77115766686954	39.0	36.0	40.0	30.0	41.0
28	36.65700788438848	39.0	35.0	40.0	30.0	41.0
29	36.53769800429781	38.0	35.0	40.0	30.0	41.0
30	36.519947046853495	38.0	35.0	40.0	30.0	41.0
31	36.45311594528745	38.0	35.0	40.0	30.0	41.0
32	36.333932890547665	38.0	35.0	40.0	30.0	41.0
33	36.31034321694957	38.0	35.0	40.0	30.0	41.0
34	36.300916024201	38.0	35.0	40.0	30.0	41.0
35	36.18577428064218	38.0	35.0	40.0	30.0	41.0
36	36.11947454721292	38.0	35.0	40.0	30.0	41.0
37	35.97306552548129	38.0	35.0	40.0	29.0	41.0
38	35.927567207935944	38.0	35.0	40.0	29.0	41.0
39	35.82198977417171	38.0	34.0	40.0	28.0	41.0
40	35.753993177714456	38.0	34.0	40.0	28.0	41.0
41	35.6912524707985	38.0	34.0	40.0	28.0	41.0
42	35.64612370427558	38.0	34.0	40.0	28.0	41.0
43	34.314188194254	37.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	19.0
11	16.0
12	10.0
13	8.0
14	7.0
15	14.0
16	32.0
17	95.0
18	239.0
19	528.0
20	1221.0
21	2552.0
22	4897.0
23	8800.0
24	15227.0
25	24128.0
26	38080.0
27	56521.0
28	82111.0
29	113052.0
30	153149.0
31	200348.0
32	259116.0
33	333269.0
34	432130.0
35	568355.0
36	765492.0
37	1052433.0
38	1457557.0
39	746364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.22340515142092	18.626511533348573	12.912404057651225	26.237679257579277
2	18.485752810582774	20.813781751462844	35.686072898135016	25.014392539819358
3	20.081883893212105	22.616918406807592	29.652020370589693	27.64917732939061
4	15.36448750841544	15.719804324791964	33.598505455865094	35.317202710927496
5	15.685097562103255	35.40485628676906	33.65324144063465	15.256804710493032
6	35.56103671886825	33.90736714371577	15.09609593253208	15.435500204883901
7	29.322511958949754	29.982542118366528	21.366999948699856	19.327945973983862
8	26.22789423050737	35.076424544774575	19.559635781658997	19.13604544305906
9	26.671782958599522	14.926441296487585	19.538355722784168	38.86342002212872
10	17.37521557142975	26.391326349336232	32.84749671140757	23.385961367826454
11	35.69210541482498	21.73498346678759	21.30309643856681	21.269814679820616
12	21.45862770220331	24.68472579440803	30.313697201228923	23.542949302159734
13	30.878679597680218	19.67569443608794	24.260802954888174	25.184823011343667
14	22.28869249871591	20.447318237580458	25.64807595967682	31.61591330402681
15	25.190459693605156	27.304611376091476	22.669675219434772	24.8352537108686
16	24.405583355447284	26.036088699845404	24.733144261699124	24.82518368300819
17	24.061824904382235	26.062942107473162	25.550906524174717	24.324326463969882
18	24.107203363262638	25.3160183743175	26.287807729601386	24.288970532818475
19	25.061481003430774	25.125020345889627	26.499753315984176	23.313745334695426
20	24.491289425900746	24.93235981286215	26.484885774850643	24.091464986386462
21	24.125665081006726	25.32116422188768	26.722370598558655	23.830800098546938
22	24.67912077890082	24.90962308329053	26.024324500631117	24.38693163717753
23	24.11659255590607	25.183904675469606	26.2290025669071	24.470500201717226
24	24.109610036587767	25.118655328279743	26.281062710940166	24.490671924192323
25	24.477751888446893	24.898096384733332	26.11408391563217	24.51006781118761
26	24.084181632902517	25.277147433441232	26.34466538690757	24.29400554674868
27	24.442649291329708	25.536355650583964	25.9954602541067	24.025534803979625
28	24.17226270992683	25.58944496413107	25.96080099154941	24.277491334392693
29	24.093206657871754	25.71174196915278	26.354545414242313	23.840505958733154
30	24.426372579630804	25.479133825603583	26.003456109561903	24.09103748520371
31	24.18635441558055	25.02848424547308	26.431416460252205	24.353744878694165
32	23.801967518776802	25.529024796968603	26.271990185839517	24.39701749841508
33	23.912104490155755	25.01596004415612	26.318366147479676	24.753569318208445
34	24.18010023161064	24.966480740596715	26.756792360458864	24.09662666733378
35	24.01649394563325	24.93555815504423	26.594136077109816	24.45381182221271
36	23.76534491745427	24.621740620149048	26.99353301544134	24.619381446955337
37	23.56049268402976	24.323835629278577	27.326192269131944	24.789479417559722
38	23.501370853792697	24.167275196128042	27.65161566947002	24.679738280609243
39	23.14877737828408	24.16589769231695	28.1976771802402	24.48764774915877
40	23.418958125784147	24.015813110416275	28.230357270655116	24.334871493144465
41	22.748652104604155	24.21111781742596	28.384780197891878	24.655449880078002
42	22.46840132924368	24.463628516038895	28.27877573794621	24.78919441677122
43	22.232230675838203	24.202092792456725	28.216265565001397	25.349410966703672
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5469.0
1	4477.5
2	3486.0
3	6017.0
4	8548.0
5	8548.0
6	9874.0
7	11200.0
8	10230.0
9	9260.0
10	12422.0
11	15584.0
12	15584.0
13	25519.5
14	35455.0
15	44300.0
16	53145.0
17	48851.5
18	44558.0
19	44558.0
20	49883.0
21	55208.0
22	48166.5
23	41125.0
24	48148.0
25	55171.0
26	55171.0
27	66365.0
28	77559.0
29	89233.0
30	100907.0
31	116576.0
32	132245.0
33	132245.0
34	152472.5
35	172700.0
36	197926.0
37	223152.0
38	252052.5
39	280953.0
40	280953.0
41	311936.5
42	342920.0
43	375755.0
44	408590.0
45	441873.0
46	475156.0
47	475156.0
48	506668.0
49	538180.0
50	553048.5
51	567917.0
52	560794.0
53	553671.0
54	553671.0
55	526917.5
56	500164.0
57	468615.0
58	437066.0
59	398613.5
60	360161.0
61	360161.0
62	316439.0
63	272717.0
64	231957.0
65	191197.0
66	159274.5
67	127352.0
68	127352.0
69	104989.5
70	82627.0
71	67834.5
72	53042.0
73	43232.0
74	33422.0
75	33422.0
76	26903.5
77	20385.0
78	16401.0
79	12417.0
80	9519.5
81	6622.0
82	6622.0
83	5109.0
84	3596.0
85	2743.0
86	1890.0
87	1309.0
88	728.0
89	728.0
90	447.0
91	166.0
92	104.0
93	42.0
94	28.0
95	14.0
96	14.0
97	9.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6315772.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.25848219939946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.637279345221	45.365309446511176
2	9.874048477663445	11.109979610332726
3	3.4313973111252394	5.791356136410194
4	1.6454762985151072	3.702879961981835
5	0.9853916273835415	2.7718318664297117
6	0.6508233748375846	2.1968601148952005
7	0.4647950566451661	1.8304065094448658
8	0.3540529038354676	1.5934783190458675
9	0.26611651572359035	1.3474180136521634
>10	1.5653587005673961	16.13107448464284
>50	0.09344645482469467	3.5575877392016966
>100	0.030885247275517683	2.841588517932845
>500	5.347031094988121E-4	0.21329334710671485
>1k	2.814166234166591E-4	0.4367277087939791
>5k	0.0	0.0
>10k+	1.1256664934039415E-4	1.1102082236182864
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21719	0.34388511808216005	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21024	0.332880920970548	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16261	0.257466545657443	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11120	0.17606715378579216	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5833377139010084E-5	3.166675427802017E-5
2	0.0	0.0	0.0	1.5833377139010084E-5	3.166675427802017E-5
3	0.0	0.0	0.0	1.5833377139010084E-5	3.166675427802017E-5
4	0.0	0.0	0.0	1.5833377139010084E-5	4.7500131417030255E-5
5	0.0	0.0	0.0	1.5833377139010084E-5	6.333350855604034E-5
6	0.0	0.0	0.0	1.5833377139010084E-5	7.916688569505042E-5
7	0.0	0.0	0.0	1.5833377139010084E-5	1.1083363997307059E-4
8	0.0	0.0	0.0	1.5833377139010084E-5	1.2666701711208067E-4
9	0.0	0.0	0.0	1.5833377139010084E-5	1.4250039425109075E-4
10	0.0	0.0	0.0	1.5833377139010084E-5	1.4250039425109075E-4
11	1.5833377139010084E-5	0.0	0.0	3.166675427802017E-5	1.4250039425109075E-4
12	1.5833377139010084E-5	0.0	0.0	7.916688569505042E-5	1.4250039425109075E-4
13	3.166675427802017E-5	0.0	0.0	9.500026283406051E-5	1.4250039425109075E-4
14	3.166675427802017E-5	0.0	0.0	1.2666701711208067E-4	2.5333403422416134E-4
15	3.166675427802017E-5	0.0	0.0	1.4250039425109075E-4	3.008341656411916E-4
16	3.166675427802017E-5	0.0	0.0	1.5833377139010083E-4	3.6416767419723194E-4
17	3.166675427802017E-5	0.0	0.0	3.325009199192118E-4	3.8000105133624204E-4
18	3.166675427802017E-5	0.0	0.0	3.6416767419723194E-4	4.2750118275327226E-4
19	3.166675427802017E-5	0.0	0.0	4.908346913093126E-4	4.4333455989228237E-4
20	3.166675427802017E-5	0.0	0.0	7.125019712554538E-4	4.4333455989228237E-4
21	4.7500131417030255E-5	0.0	0.0	0.0011875032854257563	4.591679370312925E-4
22	4.7500131417030255E-5	0.0	0.0	0.002248339553739432	4.750013141703025E-4
23	4.7500131417030255E-5	0.0	0.0	0.002818341130743795	5.70001577004363E-4
24	4.7500131417030255E-5	1.5833377139010084E-5	0.0	0.0044650123532008435	5.70001577004363E-4
25	4.7500131417030255E-5	1.5833377139010084E-5	0.0	0.006048350067101852	6.016683312823832E-4
26	6.333350855604034E-5	1.5833377139010084E-5	0.0	0.00932585913487694	6.016683312823832E-4
27	6.333350855604034E-5	1.5833377139010084E-5	0.0	0.030669251518262535	6.175017084213933E-4
28	6.333350855604034E-5	1.5833377139010084E-5	0.0	0.09621943287376429	6.175017084213933E-4
29	6.333350855604034E-5	1.5833377139010084E-5	0.0	0.19104552855929569	6.175017084213933E-4
30	7.916688569505042E-5	1.5833377139010084E-5	0.0	0.3110783606501311	6.175017084213933E-4
31	7.916688569505042E-5	1.5833377139010084E-5	0.0	0.6580509872743981	6.175017084213933E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4910	0.0	17.143585	37
GGTATCA	10010	0.0	15.931068	1
TCTTATA	7875	0.0	13.08508	37
TAACACG	175	5.316015E-7	12.685714	4
CTCTTAT	11555	0.0	12.327997	37
TCGATTA	340	1.8189894E-12	11.426471	13
GTATCAA	14175	0.0	11.276191	2
TCGTTAC	660	0.0	11.212121	23
TATACTG	930	0.0	11.139785	5
TTATACT	1130	0.0	10.805309	4
CTATACT	755	0.0	10.781457	4
TAATCGA	395	0.0	10.772152	20
TCTCTTA	16550	0.0	10.731118	37
GTATAAC	640	0.0	10.6953125	1
GTCTAGG	745	0.0	10.677852	1
GGGTATA	695	0.0	10.647482	1
CTCGTTA	875	0.0	10.571428	22
TAGATCG	280	1.2687451E-8	10.571428	5
CTAGAGT	1020	0.0	10.519608	4
TAGGACT	900	0.0	10.483334	4
>>END_MODULE