FastQCFastQC Report
Thu 9 Feb 2017
SRR2727136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4938195
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT204630.41438217810353783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT195170.3952253809337217No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT149730.30320795351337887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110670.22411022650988874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98800.015.85981751
CTTATAC41400.015.77415537
TATACCG2250.015.6222225
TCTATAC4750.013.6315783
TCTTATA66650.013.18454637
TAAGAGT7550.012.4966894
CTCTTAT100650.012.44361737
CGCGAAT1951.4705256E-712.33333428
TATACAC17700.012.33333337
GTATCAA132500.011.7701892
TGTACTG12300.011.5813015
TATACTG7300.011.404115
TAATACG1951.96383E-611.3846164
GTATAGG6300.010.865081
AACGACG3251.0004442E-1010.81538515
AACGCAG139000.010.7805767
TATACTA5300.010.4716985
CTATACT7250.010.46206954
TTATACA24950.010.4549137
CGTATAC1604.8269756E-410.406253
Produced by FastQC (version 0.11.5)