##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727135.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5425663
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.626002941944606	31.0	31.0	34.0	30.0	34.0
2	31.87393430074813	31.0	31.0	34.0	30.0	34.0
3	31.991125139913777	33.0	31.0	34.0	30.0	34.0
4	35.7170618595368	37.0	35.0	37.0	35.0	37.0
5	35.314795260966264	37.0	35.0	37.0	33.0	37.0
6	35.32614889645745	37.0	35.0	37.0	32.0	37.0
7	35.281723358048595	37.0	35.0	37.0	32.0	37.0
8	35.2335572629557	37.0	35.0	37.0	32.0	37.0
9	37.06755008558401	39.0	37.0	39.0	33.0	39.0
10	36.83358586775478	39.0	37.0	39.0	33.0	39.0
11	36.95071920242743	39.0	37.0	39.0	33.0	39.0
12	36.82860951739907	39.0	37.0	39.0	33.0	39.0
13	36.959467994971305	39.0	37.0	39.0	33.0	39.0
14	38.011227383639564	40.0	37.0	41.0	33.0	41.0
15	38.0138628219261	40.0	37.0	41.0	33.0	41.0
16	37.96271349694959	40.0	37.0	41.0	33.0	41.0
17	37.86539672663046	39.0	37.0	41.0	33.0	41.0
18	37.886550086136936	39.0	37.0	41.0	33.0	41.0
19	37.927098863309425	40.0	37.0	41.0	33.0	41.0
20	37.80336135878694	39.0	37.0	41.0	33.0	41.0
21	37.798650413783534	39.0	37.0	41.0	33.0	41.0
22	37.70625156778075	39.0	37.0	41.0	32.0	41.0
23	37.65157843382458	39.0	37.0	40.0	32.0	41.0
24	37.55252823479822	39.0	37.0	41.0	32.0	41.0
25	37.52725740614557	39.0	37.0	40.0	32.0	41.0
26	37.39462753215598	39.0	36.0	40.0	32.0	41.0
27	37.210326369330346	39.0	36.0	40.0	31.0	41.0
28	37.10494698988861	39.0	36.0	40.0	31.0	41.0
29	37.02567575612418	39.0	36.0	40.0	31.0	41.0
30	36.97134082968294	39.0	36.0	40.0	31.0	41.0
31	36.934829347123106	39.0	36.0	40.0	31.0	41.0
32	36.83579647316835	39.0	36.0	40.0	30.0	41.0
33	36.81022356161819	39.0	35.0	40.0	30.0	41.0
34	36.80455033790341	39.0	35.0	40.0	30.0	41.0
35	36.71538943719874	39.0	35.0	40.0	30.0	41.0
36	36.63885372165577	39.0	35.0	40.0	30.0	41.0
37	36.508392246256356	38.0	35.0	40.0	30.0	41.0
38	36.46297936307508	38.0	35.0	40.0	30.0	41.0
39	36.36607452397983	38.0	35.0	40.0	30.0	41.0
40	36.301931763915306	38.0	35.0	40.0	30.0	41.0
41	36.25052440595739	38.0	35.0	40.0	30.0	41.0
42	36.19373448000733	38.0	35.0	40.0	30.0	41.0
43	34.854927775646956	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	5.0
11	6.0
12	7.0
13	5.0
14	7.0
15	8.0
16	28.0
17	41.0
18	121.0
19	298.0
20	637.0
21	1476.0
22	2771.0
23	5212.0
24	9189.0
25	15107.0
26	24206.0
27	37061.0
28	54749.0
29	77918.0
30	107797.0
31	144743.0
32	192125.0
33	251534.0
34	333827.0
35	449541.0
36	622795.0
37	886443.0
38	1333960.0
39	874041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.554670277162444	18.42803358778457	12.684256283517792	26.333039851535194
2	18.455919580703778	20.56423334807193	35.76978518569989	25.210061885524404
3	20.035173581551234	22.36226245529809	29.722948144770513	27.87961581838017
4	15.2066761241898	15.527429551006025	33.670594727317194	35.59529959748698
5	15.639895069045018	35.25725796091648	33.77546670333192	15.327380266706575
6	35.64589249277001	33.73458690670615	15.105766797532393	15.51375380299145
7	29.295571803851438	29.92766045366253	21.274174234558984	19.50259350792705
8	26.132677241472607	34.96632577438001	19.625712101912708	19.27528488223467
9	26.535595741939737	14.994960062945303	19.569682083092886	38.89976211202207
10	17.370485413487717	26.364446151557885	32.97362921360947	23.29143922134493
11	35.69055062948067	21.693385674709248	21.294116497836303	21.321947197973778
12	21.461506179060514	24.584553076739194	30.28243737217	23.671503372030294
13	30.84212933976917	19.72601320797108	24.195439340777337	25.23641811148241
14	22.307688479730494	20.56790110259336	25.49601772170516	31.62839269597098
15	25.266313075471146	27.253443496214196	22.62847876840121	24.851764659913453
16	24.498830834130317	26.016857294675322	24.710952375774166	24.77335949542019
17	24.098750696458662	26.117250555369914	25.554738655902515	24.22926009226891
18	24.134727866437704	25.318896510896455	26.274447933828547	24.27192768883729
19	24.960949472903128	25.217065637876885	26.465244892651828	23.356739996568162
20	24.50284877626937	25.016297547414943	26.393124674348556	24.087729001967134
21	24.161378987231608	25.322103492236803	26.611180974564768	23.905336545966822
22	24.747722075624676	24.90444024997498	25.903046318947563	24.44479135545278
23	24.174262205374717	25.127048989220302	26.097013397256703	24.601675408148278
24	24.119632937025393	25.118791933815277	26.165705463092714	24.595869666066616
25	24.547156725362413	24.862528321423575	25.94847855460245	24.64183639861156
26	24.114490708324492	25.222668639758865	26.25885536938066	24.403985282535977
27	24.480897541922523	25.471670466816683	25.908077962085002	24.139354029175788
28	24.224799807876014	25.508237426467513	25.894752401688052	24.37221036396842
29	24.133898474711753	25.627209061823415	26.274927137936878	23.96396532552796
30	24.41250037092241	25.427104484742237	26.00550384349341	24.154891300841943
31	24.309932260813103	24.973242901374448	26.27271542666767	24.444109411144776
32	23.903769917151138	25.40012160725795	26.150352500698993	24.545755974891918
33	23.967227599650034	24.9474211723065	26.247520349125995	24.837830878917472
34	24.30482689396669	24.889971972088944	26.561730796770828	24.24347033717354
35	24.066496573782782	24.86090639982616	26.54711507146684	24.52548195492422
36	23.841860432540688	24.574397635828102	26.853547667814976	24.730194263816237
37	23.608451169930753	24.225997818146833	27.296922053581284	24.868628958341127
38	23.567479218668762	24.1100304239316	27.652104452488114	24.67038590491153
39	23.22130954318394	24.08135190114093	28.15379429205242	24.543544263622714
40	23.431919748793835	23.910976409703295	28.24513796747052	24.411965874032354
41	22.929160915449412	24.039476834444013	28.328888100864358	24.70247414924222
42	22.574457720650916	24.307517809344223	28.330933933788366	24.787090536216496
43	22.431138830406532	24.068210650016415	28.19279413409937	25.307856385477685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4823.0
1	3935.5
2	3048.0
3	5087.5
4	7127.0
5	7127.0
6	7871.5
7	8616.0
8	7650.0
9	6684.0
10	9098.0
11	11512.0
12	11512.0
13	18861.5
14	26211.0
15	31933.0
16	37655.0
17	35379.5
18	33104.0
19	33104.0
20	38605.5
21	44107.0
22	39547.0
23	34987.0
24	41464.0
25	47941.0
26	47941.0
27	57852.5
28	67764.0
29	78077.0
30	88390.0
31	102578.5
32	116767.0
33	116767.0
34	133699.0
35	150631.0
36	172654.0
37	194677.0
38	218590.5
39	242504.0
40	242504.0
41	269283.5
42	296063.0
43	324215.0
44	352367.0
45	380210.0
46	408053.0
47	408053.0
48	435451.5
49	462850.0
50	476484.5
51	490119.0
52	486484.0
53	482849.0
54	482849.0
55	458625.5
56	434402.0
57	404682.5
58	374963.0
59	341703.0
60	308443.0
61	308443.0
62	270796.0
63	233149.0
64	198595.5
65	164042.0
66	136396.5
67	108751.0
68	108751.0
69	89309.0
70	69867.0
71	57477.5
72	45088.0
73	36844.5
74	28601.0
75	28601.0
76	23034.5
77	17468.0
78	14152.0
79	10836.0
80	8230.0
81	5624.0
82	5624.0
83	4402.0
84	3180.0
85	2356.5
86	1533.0
87	1103.5
88	674.0
89	674.0
90	407.0
91	140.0
92	85.5
93	31.0
94	24.5
95	18.0
96	18.0
97	11.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5425663.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.3201261901348
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72095327575771	47.076561812301655
2	10.10106640352582	11.781909346171139
3	3.4084942214997493	5.963514393486319
4	1.6477146151771191	3.8437969712983597
5	0.9611128650272941	2.8026111785676897
6	0.6169148926878584	2.1587132634077624
7	0.44842586253673433	1.8306577023043673
8	0.3180671037627269	1.4839770902698346
9	0.2506124015952721	1.3154172197264107
>10	1.4261254541205561	15.024177573412986
>50	0.07650378253793585	2.9981360019198102
>100	0.023124316997394177	2.143245858401061
>500	4.424040883617846E-4	0.17797174028265644
>1k	3.1600048968310557E-4	0.4135509884342044
>5k	3.1600048963330415E-5	0.15712726056746928
>10k+	9.480014688999123E-5	0.8286315994481729
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17546	0.3233890494120258	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15206	0.2802606796625592	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12211	0.2250600525686907	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8526	0.15714208567690252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.686185448672356E-5
2	0.0	0.0	0.0	0.0	3.686185448672356E-5
3	0.0	0.0	0.0	0.0	3.686185448672356E-5
4	0.0	0.0	0.0	0.0	3.686185448672356E-5
5	0.0	0.0	0.0	0.0	3.686185448672356E-5
6	0.0	0.0	0.0	0.0	5.529278173008534E-5
7	0.0	0.0	0.0	1.843092724336178E-5	7.372370897344711E-5
8	0.0	0.0	0.0	1.843092724336178E-5	7.372370897344711E-5
9	0.0	0.0	0.0	5.529278173008534E-5	1.2901649070353244E-4
10	0.0	0.0	0.0	5.529278173008534E-5	1.2901649070353244E-4
11	0.0	0.0	0.0	5.529278173008534E-5	1.2901649070353244E-4
12	0.0	1.843092724336178E-5	0.0	5.529278173008534E-5	1.2901649070353244E-4
13	0.0	1.843092724336178E-5	0.0	5.529278173008534E-5	1.6587834519025602E-4
14	0.0	1.843092724336178E-5	0.0	5.529278173008534E-5	2.0274019967697957E-4
15	0.0	1.843092724336178E-5	0.0	7.372370897344711E-5	2.7646390865042667E-4
16	0.0	1.843092724336178E-5	0.0	1.4744741794689423E-4	3.1332576313715025E-4
17	0.0	1.843092724336178E-5	0.0	2.0274019967697957E-4	3.3175669038051204E-4
18	0.0	1.843092724336178E-5	0.0	2.580329814070649E-4	3.501876176238738E-4
19	1.843092724336178E-5	1.843092724336178E-5	0.0	3.501876176238738E-4	3.501876176238738E-4
20	1.843092724336178E-5	1.843092724336178E-5	0.0	4.423422538406827E-4	3.501876176238738E-4
21	1.843092724336178E-5	1.843092724336178E-5	0.0	8.293917259512801E-4	3.8704947211059735E-4
22	1.843092724336178E-5	1.843092724336178E-5	0.0	0.0012901649070353246	3.8704947211059735E-4
23	1.843092724336178E-5	1.843092724336178E-5	0.0	0.001750938088119369	4.239113265973209E-4
24	1.843092724336178E-5	1.843092724336178E-5	0.0	0.002672484450287458	4.239113265973209E-4
25	1.843092724336178E-5	1.843092724336178E-5	0.0	0.0041469586297564	4.239113265973209E-4
26	1.843092724336178E-5	1.843092724336178E-5	0.0	0.007390801824588073	4.239113265973209E-4
27	1.843092724336178E-5	1.843092724336178E-5	0.0	0.028844401135861186	4.239113265973209E-4
28	1.843092724336178E-5	1.843092724336178E-5	0.0	0.0860724302264995	4.239113265973209E-4
29	1.843092724336178E-5	1.843092724336178E-5	0.0	0.16731595751523823	4.239113265973209E-4
30	1.843092724336178E-5	3.686185448672356E-5	0.0	0.2736439767821923	4.239113265973209E-4
31	1.843092724336178E-5	3.686185448672356E-5	0.0	0.5837074657972675	4.239113265973209E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	4035	0.0	16.047089	37
GGTATCA	7905	0.0	15.141683	1
TCTTATA	6275	0.0	13.473307	37
GTATTAG	545	0.0	12.899082	1
TATACAC	1665	0.0	12.666667	37
TATACCG	190	1.0482654E-7	12.657895	5
TCTACAC	885	0.0	12.333333	3
CTCTTAT	9385	0.0	11.8076725	37
GTATTAC	420	0.0	11.45238	1
GTATCAA	10665	0.0	11.309893	2
TCTCTTA	13435	0.0	11.277634	37
GTCTTAC	560	0.0	11.232142	1
TCTATAC	445	0.0	11.224718	3
CGCAATC	220	7.0216083E-7	10.931818	22
CTATACT	610	0.0	10.918033	4
TAGACTG	825	0.0	10.763637	5
CGTTATA	155	3.600842E-4	10.741936	2
TATACTG	745	0.0	10.677852	5
ACCGTAT	195	2.4374463E-5	10.435898	8
CTTACAC	1065	0.0	10.422535	3
>>END_MODULE