FastQCFastQC Report
Thu 9 Feb 2017
SRR2727134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12354876
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT305310.24711700870166564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT270490.21893380394914524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT216980.17562296861579185No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153080.12390249809063239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133800.017.2832581
CTTATAC92450.016.46890337
TCTTATA151250.014.38413137
GTATCAA184250.012.5308012
CTCTTAT232100.012.4980637
TATACTG17900.012.0921785
TTATACT20700.011.6183584
AACGCAG200550.011.2632757
TCTCTTA326050.010.55926937
CGTATAC3709.094947E-1210.53
CTATACT16750.010.49253754
GTATTAT12550.010.4661351
TCGTTAC13650.010.43589823
TATACAC41550.010.41877337
GGGTAAG44400.010.3751
TACTTAC25450.010.32220131
CTCGTTA14950.010.27090422
GTTACGG19600.010.19387725
GTGTTAG14300.010.0909091
ATCAACG229750.010.0491844
Produced by FastQC (version 0.11.5)