##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727134.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12354876
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05734545615836	33.0	31.0	34.0	30.0	34.0
2	32.28380899978276	34.0	31.0	34.0	30.0	34.0
3	32.39779525104097	34.0	31.0	34.0	30.0	34.0
4	35.99750292920787	37.0	35.0	37.0	35.0	37.0
5	35.707395444519236	37.0	35.0	37.0	33.0	37.0
6	35.71850449976188	37.0	35.0	37.0	33.0	37.0
7	35.682883907535775	37.0	35.0	37.0	33.0	37.0
8	35.649719025913335	37.0	35.0	37.0	33.0	37.0
9	37.4795411139699	39.0	37.0	39.0	35.0	39.0
10	37.303073863307084	39.0	37.0	39.0	34.0	39.0
11	37.391386364379535	39.0	37.0	39.0	34.0	39.0
12	37.296890798418374	39.0	37.0	39.0	34.0	39.0
13	37.36758029785163	39.0	37.0	39.0	34.0	39.0
14	38.583675546399654	40.0	38.0	41.0	34.0	41.0
15	38.53409463599635	40.0	38.0	41.0	34.0	41.0
16	38.50019037018259	40.0	38.0	41.0	34.0	41.0
17	38.44572110638747	40.0	38.0	41.0	34.0	41.0
18	38.44320509570473	40.0	38.0	41.0	34.0	41.0
19	38.489608556168434	40.0	38.0	41.0	34.0	41.0
20	38.40913490349883	40.0	38.0	41.0	34.0	41.0
21	38.35341900639067	40.0	38.0	41.0	34.0	41.0
22	38.299408751654006	40.0	38.0	41.0	34.0	41.0
23	38.24541840808439	40.0	38.0	41.0	34.0	41.0
24	38.175597229790085	40.0	38.0	41.0	33.0	41.0
25	38.14267856674563	40.0	38.0	41.0	33.0	41.0
26	37.99518837744709	40.0	37.0	41.0	33.0	41.0
27	37.87459291376133	40.0	37.0	41.0	33.0	41.0
28	37.8025559301445	40.0	37.0	41.0	33.0	41.0
29	37.718829715490465	40.0	37.0	41.0	33.0	41.0
30	37.673770096923676	40.0	37.0	41.0	33.0	41.0
31	37.634380061766706	40.0	37.0	41.0	32.0	41.0
32	37.541416441573354	39.0	37.0	41.0	32.0	41.0
33	37.47405607308402	39.0	37.0	41.0	32.0	41.0
34	37.44141446664459	39.0	37.0	41.0	32.0	41.0
35	37.37820428145131	39.0	37.0	41.0	32.0	41.0
36	37.271522919372075	39.0	36.0	41.0	31.0	41.0
37	37.2011771708595	39.0	36.0	41.0	31.0	41.0
38	37.170687508316554	39.0	36.0	41.0	31.0	41.0
39	37.08446543696594	39.0	36.0	41.0	31.0	41.0
40	37.012519105817006	39.0	36.0	40.0	31.0	41.0
41	36.969619282297934	39.0	36.0	40.0	31.0	41.0
42	36.849106700868546	39.0	36.0	40.0	31.0	41.0
43	35.55205952694305	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	10.0
11	8.0
12	8.0
13	9.0
14	5.0
15	17.0
16	51.0
17	96.0
18	239.0
19	566.0
20	1274.0
21	2688.0
22	5145.0
23	9227.0
24	16271.0
25	26923.0
26	41605.0
27	62909.0
28	91692.0
29	131197.0
30	180625.0
31	243385.0
32	323082.0
33	430800.0
34	583717.0
35	808821.0
36	1165528.0
37	1817510.0
38	3279645.0
39	3131818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.947221809429735	17.984348851417042	12.361402898742165	26.70702644041106
2	18.53178453591926	20.125017847204617	35.77808470113338	25.565112915742738
3	20.057570792292857	21.89824487109381	29.560741847995885	28.48344248861745
4	15.159318474746328	15.25325709460783	33.62525046791242	35.96217396273342
5	15.764706986941837	35.092849171452634	33.756728922248996	15.385714919356536
6	36.05297212209981	33.54617237761027	15.058637577584753	15.342217922705172
7	29.721229092060497	29.64891756096945	21.040866780047004	19.58898656692305
8	26.391466818444798	34.7680462353487	19.422056522461254	19.41843042374525
9	26.630408917094755	14.832694395314045	19.351525664846818	39.185371022744384
10	17.42459414404483	26.155333327505677	32.94467706515225	23.475395463297243
11	36.03260769270367	21.464675161450426	21.15147897882585	21.35123816702005
12	21.582928068237997	24.334295220769516	30.155422037420692	23.927354673571795
13	31.00544270942096	19.520228288814877	23.933878413672463	25.540450588091694
14	22.493791115345875	20.27454585541773	25.121506682867555	32.11015634636883
15	25.33039586961456	27.127354414564742	22.252639362790852	25.28961035302985
16	24.88512227884764	25.804516370702547	24.26490561297418	25.04545573747563
17	24.30385379828984	25.891915062522685	25.188824234253747	24.615406904933728
18	24.42800720946127	25.200625243021456	25.81358161749256	24.55778593002471
19	25.2506136038921	25.04547192541633	25.951057703857167	23.752856766834405
20	24.82542115355913	24.846400724701727	25.88790854720031	24.44026957453883
21	24.521735386093717	25.125148969524258	26.066655788370518	24.286459856011504
22	25.10327096767301	24.726229546941628	25.445767322958158	24.724732162427205
23	24.54337866280487	24.974738718543186	25.57391106151126	24.90797155714068
24	24.472661643872428	24.934616907527037	25.6510385049595	24.941682943641037
25	24.878007678911548	24.68951529744208	25.476087335882607	24.95638968776376
26	24.485960037154562	25.066742879491464	25.718720285011358	24.728576798342612
27	24.76949181845289	25.30216410103995	25.422845198931988	24.50549888157518
28	24.54790319222953	25.29014455507283	25.407466655270355	24.754485597427283
29	24.46854181296518	25.476823887184295	25.764839728055545	24.289794571794975
30	24.770665444153387	25.205344027734476	25.514209936222755	24.50978059188939
31	24.661024521816326	24.774760993149584	25.798793933666353	24.765420551367736
32	24.236568622785047	25.19317879030109	25.717012457267884	24.85324012964598
33	24.249891297978223	24.74806707894114	25.810999640951476	25.191041982129157
34	24.586276705650466	24.62141263093211	26.144552159001837	24.647758504415584
35	24.381628759365938	24.59305945280228	26.11112406146367	24.914187726368116
36	24.16144848398317	24.28671886306265	26.513289166155936	25.038543486798247
37	23.953797674699448	23.927726996207813	26.929383993817503	25.189091335275236
38	23.812112723753764	23.856297707884725	27.284288405646485	25.04730116271503
39	23.464209596276	23.80310413475619	27.83404705963864	24.898639209329172
40	23.69355224609296	23.64290017965377	27.88677118248698	24.776776391766294
41	23.089515426945603	23.821785018319893	28.03854122048655	25.050158334247953
42	22.73389065175563	24.096931446337464	28.036355848492526	25.132822053414376
43	22.629025171924024	23.80503049969907	27.930041547968592	25.63590278040832
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6749.0
1	5624.0
2	4499.0
3	7985.5
4	11472.0
5	11472.0
6	13230.5
7	14989.0
8	13398.0
9	11807.0
10	16366.0
11	20925.0
12	20925.0
13	33962.0
14	46999.0
15	57707.0
16	68415.0
17	64623.5
18	60832.0
19	60832.0
20	70978.5
21	81125.0
22	74579.5
23	68034.0
24	81811.5
25	95589.0
26	95589.0
27	116656.0
28	137723.0
29	160423.0
30	183123.0
31	214676.5
32	246230.0
33	246230.0
34	287777.5
35	329325.0
36	380074.0
37	430823.0
38	488650.5
39	546478.0
40	546478.0
41	608902.0
42	671326.0
43	739792.5
44	808259.0
45	873872.0
46	939485.0
47	939485.0
48	1002949.0
49	1066413.0
50	1096668.0
51	1126923.0
52	1117712.5
53	1108502.0
54	1108502.0
55	1061367.0
56	1014232.0
57	949934.0
58	885636.0
59	809729.0
60	733822.0
61	733822.0
62	644476.5
63	555131.0
64	472511.0
65	389891.0
66	325177.5
67	260464.0
68	260464.0
69	213918.5
70	167373.0
71	136560.0
72	105747.0
73	86267.0
74	66787.0
75	66787.0
76	53663.0
77	40539.0
78	32295.5
79	24052.0
80	18378.0
81	12704.0
82	12704.0
83	9821.5
84	6939.0
85	5154.0
86	3369.0
87	2401.5
88	1434.0
89	1434.0
90	959.0
91	484.0
92	313.5
93	143.0
94	97.5
95	52.0
96	52.0
97	35.5
98	19.0
99	16.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.2354876E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.58305692720487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.84577188529487	38.102482820035746
2	11.354666725654097	11.259981732950921
3	4.158813964908999	6.186201287152125
4	2.107605227485464	4.180060398979454
5	1.2509431134649842	3.1012791803814603
6	0.7993477504210441	2.378046300825587
7	0.5717993487285421	1.984609176126218
8	0.4390820360324328	1.741682367064731
9	0.3319300154736369	1.4812294367769596
>10	1.9268227339900543	17.964421249464547
>50	0.1504435551521341	5.1201942462221846
>100	0.06123369413542244	4.972596405520159
>500	0.0011808522524746862	0.38333843290091263
>1k	2.4484048022387224E-4	0.24128305562173216
>5k	4.896708247261654E-5	0.1370975452810435
>10k+	6.528944329682204E-5	0.76549636469615
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30531	0.24711700870166564	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27049	0.21893380394914524	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21698	0.17562296861579185	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15308	0.12390249809063239	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	8.093970348225267E-6
3	0.0	0.0	0.0	0.0	8.093970348225267E-6
4	0.0	0.0	0.0	0.0	8.093970348225267E-6
5	0.0	0.0	0.0	0.0	8.093970348225267E-6
6	8.093970348225267E-6	0.0	0.0	0.0	4.046985174112634E-5
7	8.093970348225267E-6	0.0	0.0	0.0	4.8563822089351606E-5
8	8.093970348225267E-6	0.0	0.0	0.0	5.665779243757687E-5
9	8.093970348225267E-6	0.0	0.0	0.0	9.712764417870321E-5
10	8.093970348225267E-6	0.0	8.093970348225267E-6	0.0	9.712764417870321E-5
11	1.6187940696450534E-5	0.0	8.093970348225267E-6	8.093970348225267E-6	9.712764417870321E-5
12	1.6187940696450534E-5	1.6187940696450534E-5	8.093970348225267E-6	3.237588139290107E-5	9.712764417870321E-5
13	2.4281911044675803E-5	2.4281911044675803E-5	8.093970348225267E-6	3.237588139290107E-5	1.1331558487515374E-4
14	3.237588139290107E-5	2.4281911044675803E-5	8.093970348225267E-6	4.046985174112634E-5	1.8616131800918114E-4
15	4.046985174112634E-5	3.237588139290107E-5	8.093970348225267E-6	7.28457331340274E-5	2.0234925870563168E-4
16	4.8563822089351606E-5	3.237588139290107E-5	8.093970348225267E-6	1.456914662680548E-4	2.3472514009853276E-4
17	4.8563822089351606E-5	3.237588139290107E-5	8.093970348225267E-6	2.1853719940208223E-4	2.4281911044675802E-4
18	5.665779243757687E-5	3.237588139290107E-5	8.093970348225267E-6	2.913829325361096E-4	2.751949918396591E-4
19	6.475176278580214E-5	3.237588139290107E-5	8.093970348225267E-6	3.966045470630381E-4	2.913829325361096E-4
20	6.475176278580214E-5	3.237588139290107E-5	8.093970348225267E-6	5.099201319381919E-4	2.994769028843349E-4
21	7.28457331340274E-5	3.237588139290107E-5	8.093970348225267E-6	7.608332127331752E-4	3.0757087323256017E-4
22	7.28457331340274E-5	3.237588139290107E-5	8.093970348225267E-6	0.0012545654039749165	3.156648435807854E-4
23	7.28457331340274E-5	3.237588139290107E-5	8.093970348225267E-6	0.0018130493580024599	4.451683691523897E-4
24	7.28457331340274E-5	3.237588139290107E-5	8.093970348225267E-6	0.0030433328509327008	4.5326233950061497E-4
25	7.28457331340274E-5	4.046985174112634E-5	8.093970348225267E-6	0.004459777661872122	4.613563098488402E-4
26	7.28457331340274E-5	4.046985174112634E-5	8.093970348225267E-6	0.007818775356385609	4.613563098488402E-4
27	7.28457331340274E-5	4.046985174112634E-5	8.093970348225267E-6	0.031186067751711954	4.613563098488402E-4
28	7.28457331340274E-5	4.046985174112634E-5	8.093970348225267E-6	0.09425428470508324	4.6945028019706553E-4
29	7.28457331340274E-5	4.046985174112634E-5	8.093970348225267E-6	0.18111068051188858	4.6945028019706553E-4
30	7.28457331340274E-5	4.046985174112634E-5	8.093970348225267E-6	0.289885548021688	4.6945028019706553E-4
31	7.28457331340274E-5	4.046985174112634E-5	8.093970348225267E-6	0.6104472436631497	4.775442505452908E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13380	0.0	17.283258	1
CTTATAC	9245	0.0	16.468903	37
TCTTATA	15125	0.0	14.384131	37
GTATCAA	18425	0.0	12.530801	2
CTCTTAT	23210	0.0	12.49806	37
TATACTG	1790	0.0	12.092178	5
TTATACT	2070	0.0	11.618358	4
AACGCAG	20055	0.0	11.263275	7
TCTCTTA	32605	0.0	10.559269	37
CGTATAC	370	9.094947E-12	10.5	3
CTATACT	1675	0.0	10.4925375	4
GTATTAT	1255	0.0	10.466135	1
TCGTTAC	1365	0.0	10.435898	23
TATACAC	4155	0.0	10.418773	37
GGGTAAG	4440	0.0	10.375	1
TACTTAC	2545	0.0	10.322201	31
CTCGTTA	1495	0.0	10.270904	22
GTTACGG	1960	0.0	10.193877	25
GTGTTAG	1430	0.0	10.090909	1
ATCAACG	22975	0.0	10.049184	4
>>END_MODULE