FastQCFastQC Report
Thu 9 Feb 2017
SRR2727133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20382268
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT465170.2282228847152829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT421700.2068955231086158No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT341780.1676849700926315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249760.12253788440030325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA179450.019.6185571
CTTATAC136600.015.84553437
TCTTATA219450.014.45773537
GTATCAA258000.013.65988352
TCGTTAC25000.012.43223
CTCTTAT331900.012.33519137
TATACCG6950.011.4460435
CTCGTTA29550.011.01861222
AACGCAG320750.010.8894787
TATACTG28650.010.7835965
CGTTACG21200.010.73349124
GTCTTAG29300.010.4180891
TCAACGC339300.010.4140885
ATCAACG342050.010.373634
TCTATAC19500.010.3410253
TATCAAC347450.010.324223
TCTCTTA461950.010.31626837
CAACGCA344550.010.2500366
TACTTAC41650.010.21608631
TAGATCG7850.010.1337585
Produced by FastQC (version 0.11.5)