##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727133.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20382268
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.750701982723413	31.0	31.0	34.0	30.0	34.0
2	31.99063426111363	33.0	31.0	34.0	30.0	34.0
3	32.10338947559712	34.0	31.0	34.0	30.0	34.0
4	35.76852109882963	37.0	35.0	37.0	35.0	37.0
5	35.417764254694326	37.0	35.0	37.0	33.0	37.0
6	35.42751341509198	37.0	35.0	37.0	33.0	37.0
7	35.388915943996025	37.0	35.0	37.0	33.0	37.0
8	35.350511680054446	37.0	35.0	37.0	33.0	37.0
9	37.1400051750865	39.0	37.0	39.0	33.0	39.0
10	36.93804668842545	39.0	37.0	39.0	33.0	39.0
11	37.02442618260147	39.0	37.0	39.0	33.0	39.0
12	36.91882664873212	39.0	37.0	39.0	33.0	39.0
13	36.999620012846464	39.0	37.0	39.0	33.0	39.0
14	38.150410886560806	40.0	38.0	41.0	33.0	41.0
15	38.09064511368411	40.0	37.0	41.0	33.0	41.0
16	38.04741969833779	40.0	37.0	41.0	33.0	41.0
17	37.99339833035264	40.0	37.0	41.0	33.0	41.0
18	37.98440409084995	40.0	37.0	41.0	33.0	41.0
19	38.026508090267484	40.0	37.0	41.0	33.0	41.0
20	37.93814108420123	40.0	37.0	41.0	33.0	41.0
21	37.88152128114496	40.0	37.0	41.0	33.0	41.0
22	37.81831261368951	40.0	37.0	41.0	33.0	41.0
23	37.760346542396555	39.0	37.0	41.0	32.0	41.0
24	37.6693310577606	39.0	37.0	41.0	32.0	41.0
25	37.6366013831238	39.0	37.0	41.0	32.0	41.0
26	37.46630841082062	39.0	37.0	41.0	32.0	41.0
27	37.3239636531126	39.0	36.0	40.0	32.0	41.0
28	37.23841139759324	39.0	36.0	40.0	31.0	41.0
29	37.135383461742336	39.0	36.0	40.0	31.0	41.0
30	37.09652964037172	39.0	36.0	40.0	31.0	41.0
31	37.05431255245981	39.0	36.0	40.0	31.0	41.0
32	36.950202842980964	39.0	36.0	40.0	31.0	41.0
33	36.87736369671913	39.0	36.0	40.0	30.0	41.0
34	36.84079862947539	39.0	36.0	40.0	30.0	41.0
35	36.769375321725725	39.0	36.0	40.0	30.0	41.0
36	36.66067834060468	39.0	35.0	40.0	30.0	41.0
37	36.58650646728813	39.0	35.0	40.0	30.0	41.0
38	36.55475200306462	39.0	35.0	40.0	30.0	41.0
39	36.460785963564014	39.0	35.0	40.0	30.0	41.0
40	36.385694908927704	39.0	35.0	40.0	30.0	41.0
41	36.34153181579204	39.0	35.0	40.0	30.0	41.0
42	36.218979997711735	38.0	35.0	40.0	29.0	41.0
43	34.91676520002583	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	24.0
11	29.0
12	23.0
13	22.0
14	34.0
15	57.0
16	142.0
17	304.0
18	746.0
19	1583.0
20	3623.0
21	7400.0
22	14077.0
23	25177.0
24	42700.0
25	67543.0
26	103724.0
27	152240.0
28	216172.0
29	297402.0
30	397465.0
31	519654.0
32	670929.0
33	865165.0
34	1135208.0
35	1523081.0
36	2128530.0
37	3203180.0
38	5342823.0
39	3663195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.45005511653561	18.367622288157527	12.535925835142587	26.646396760164276
2	18.593509809605095	20.32236059304097	35.78382935598727	25.300300241366664
3	20.04165090950624	22.208966146456323	29.483083040611575	28.26629990342586
4	15.284133247585597	15.438507628297302	33.70996299332341	35.56739613079369
5	15.700833685436773	35.29647927306225	33.73200175760617	15.270685283894805
6	35.96390254509459	33.85014857031612	15.037914328277893	15.1480345563114
7	29.802385092767892	29.742136645441025	21.09576323890943	19.359715022881655
8	26.356222967924865	35.09772317781319	19.389716590911277	19.15633726335067
9	26.804666683805745	14.841562283451479	19.210654084226544	39.14311694851623
10	17.2992230305283	26.193581597494447	32.92401022300364	23.583185148973605
11	36.19310176865499	21.4740479322517	21.173953752349835	21.15889654674347
12	21.629168059216962	24.365060846025575	30.167575070644737	23.838196024112722
13	31.02067934736213	19.523710511509318	24.00220132519109	25.45340881593746
14	22.511508532809007	20.119929735002994	25.180490218262264	32.188071513925735
15	25.346938819566105	27.169709474922026	22.220270089668137	25.26308161584373
16	24.909543923178713	25.84437610181556	24.192974991791885	25.053104983213842
17	24.370462600138513	25.855729107280894	25.143428591950613	24.63037970062998
18	24.520077942258435	25.199781496347708	25.754513678261908	24.525626883131945
19	25.37596404875061	24.96910549895625	25.848188238914332	23.806742213378808
20	24.854162451401386	24.7974170489761	25.86027717818253	24.48814332143999
21	24.581405759162816	25.03849424411454	26.079909262305843	24.300190734416798
22	25.129951190907708	24.646629118996962	25.454507810416388	24.768911879678946
23	24.599288950572134	24.907890525234976	25.62731978600222	24.865500738190665
24	24.532976408709768	24.905648380248948	25.64415304518614	24.917222165855144
25	24.971053270421134	24.676434438012492	25.458619227261657	24.893893064304716
26	24.57867299164156	25.033013990395965	25.676965880342657	24.711347137619818
27	24.885052046219784	25.280562496774156	25.340531289255935	24.493854167750126
28	24.644205443672902	25.236244563166377	25.34687994486188	24.772670048298846
29	24.56077017533083	25.44994011461335	25.66971447927188	24.319575230783936
30	24.87460669244463	25.1691813688251	25.41949208007666	24.536719858653612
31	24.75829971424181	24.70319299108421	25.748263147163016	24.790244147510965
32	24.246928751991682	25.205919184263497	25.721352501105372	24.825799562639446
33	24.371380064279403	24.68782669328065	25.80174100350363	25.139052238936316
34	24.685942702745347	24.626709844066422	26.147688765548565	24.53965868763967
35	24.537838478033947	24.635938453954193	26.03465914588112	24.791563922130745
36	24.304316869938127	24.34603450410916	26.328021984599552	25.021626641353162
37	24.12621598342245	24.00897682240269	26.683124763151973	25.18168243102289
38	24.01551191457202	23.924643714821137	27.014348942914495	25.045495427692344
39	23.67889579314726	23.885707910424888	27.532048935869156	24.903347360558698
40	23.934583727385	23.696023425852314	27.61647035550705	24.752922491255635
41	23.3214085890736	23.873113629945404	27.785406413064536	25.020071367916465
42	23.023056119171823	24.10752817105535	27.77949441151495	25.089921298257877
43	22.872135721108172	23.822496102985202	27.644838150494344	25.660530025412285
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9746.0
1	8682.5
2	7619.0
3	13514.0
4	19409.0
5	19409.0
6	22388.5
7	25368.0
8	22247.0
9	19126.0
10	26029.5
11	32933.0
12	32933.0
13	53784.5
14	74636.0
15	89751.0
16	104866.0
17	100170.0
18	95474.0
19	95474.0
20	112246.5
21	129019.0
22	121408.5
23	113798.0
24	136846.0
25	159894.0
26	159894.0
27	195507.5
28	231121.0
29	269433.5
30	307746.0
31	360520.5
32	413295.0
33	413295.0
34	481125.5
35	548956.0
36	633694.5
37	718433.0
38	814590.5
39	910748.0
40	910748.0
41	1012104.5
42	1113461.0
43	1222268.5
44	1331076.0
45	1439504.5
46	1547933.0
47	1547933.0
48	1645326.0
49	1742719.0
50	1795358.0
51	1847997.0
52	1833559.0
53	1819121.0
54	1819121.0
55	1745048.5
56	1670976.0
57	1565117.0
58	1459258.0
59	1335741.0
60	1212224.0
61	1212224.0
62	1063438.5
63	914653.0
64	778279.0
65	641905.0
66	537023.0
67	432141.0
68	432141.0
69	355697.0
70	279253.0
71	229959.5
72	180666.0
73	146909.5
74	113153.0
75	113153.0
76	90888.0
77	68623.0
78	54769.5
79	40916.0
80	31332.0
81	21748.0
82	21748.0
83	16835.0
84	11922.0
85	9124.0
86	6326.0
87	4531.0
88	2736.0
89	2736.0
90	1801.0
91	866.0
92	571.0
93	276.0
94	187.0
95	98.0
96	98.0
97	59.5
98	21.0
99	17.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2.0382268E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.008678943426496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37412622701645	33.1711572176607
2	11.56868688664334	10.1824525398263
3	4.406633523365923	5.817903598534537
4	2.2794852161709853	4.012685321390242
5	1.3867030947971588	3.0513485644392038
6	0.8813058833711258	2.3271064603339653
7	0.6584754514102842	2.028504431327012
8	0.4612495890059451	1.623918806028005
9	0.38299991313603904	1.5169788191307763
>10	2.2741873267735158	18.923055622974392
>50	0.211303838527336	6.434362374657098
>100	0.11064750396220344	8.512596666268154
>500	0.0033897412003884944	1.0060664063645897
>1k	6.940417581451444E-4	0.4331962644877351
>5k	6.705771654192107E-5	0.23150564601322185
>10k+	4.4705144361274596E-5	0.7271612605642381
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	46517	0.2282228847152829	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	42170	0.2068955231086158	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34178	0.1676849700926315	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24976	0.12253788440030325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.9437352114102317E-5
2	0.0	0.0	0.0	9.812450704700773E-6	2.9437352114102317E-5
3	0.0	0.0	0.0	9.812450704700773E-6	2.9437352114102317E-5
4	0.0	0.0	0.0	1.9624901409401546E-5	5.396847887585425E-5
5	0.0	9.812450704700773E-6	0.0	1.9624901409401546E-5	5.396847887585425E-5
6	4.906225352350386E-6	9.812450704700773E-6	0.0	1.9624901409401546E-5	7.849960563760618E-5
7	4.906225352350386E-6	9.812450704700773E-6	0.0	1.9624901409401546E-5	7.849960563760618E-5
8	4.906225352350386E-6	9.812450704700773E-6	0.0	1.9624901409401546E-5	8.340583098995657E-5
9	4.906225352350386E-6	9.812450704700773E-6	0.0	2.453112676175193E-5	1.1284318310405888E-4
10	9.812450704700773E-6	9.812450704700773E-6	0.0	2.9437352114102317E-5	1.1774940845640927E-4
11	9.812450704700773E-6	9.812450704700773E-6	0.0	3.924980281880309E-5	1.2265563380875965E-4
12	9.812450704700773E-6	1.4718676057051158E-5	0.0	3.924980281880309E-5	1.2265563380875965E-4
13	9.812450704700773E-6	1.9624901409401546E-5	0.0	3.924980281880309E-5	1.2756185916111003E-4
14	1.4718676057051158E-5	1.9624901409401546E-5	0.0	5.396847887585425E-5	2.3549881691281853E-4
15	1.4718676057051158E-5	2.453112676175193E-5	0.0	1.0303073239935812E-4	3.1399842255042473E-4
16	1.4718676057051158E-5	2.453112676175193E-5	0.0	1.5209298592286198E-4	3.6306067607392857E-4
17	1.4718676057051158E-5	2.453112676175193E-5	0.0	2.15873915503417E-4	3.6306067607392857E-4
18	1.4718676057051158E-5	2.9437352114102317E-5	0.0	2.6002994367457045E-4	3.9740425354038125E-4
19	1.9624901409401546E-5	2.9437352114102317E-5	0.0	3.238108732551255E-4	4.0231047889273164E-4
20	1.9624901409401546E-5	2.9437352114102317E-5	0.0	4.07216704245082E-4	4.0231047889273164E-4
21	1.9624901409401546E-5	2.9437352114102317E-5	0.0	6.819653239767036E-4	4.0231047889273164E-4
22	2.453112676175193E-5	2.9437352114102317E-5	0.0	0.0010989944789264864	4.0231047889273164E-4
23	2.453112676175193E-5	2.9437352114102317E-5	0.0	0.0015111174085239189	5.200598873491409E-4
24	2.453112676175193E-5	2.9437352114102317E-5	0.0	0.0024187690987087403	5.200598873491409E-4
25	2.453112676175193E-5	2.9437352114102317E-5	0.0	0.0034588888734070223	5.396847887585424E-4
26	2.453112676175193E-5	2.9437352114102317E-5	0.0	0.005946345127048668	5.396847887585424E-4
27	2.453112676175193E-5	2.9437352114102317E-5	0.0	0.024962874592758765	5.789345915773455E-4
28	2.453112676175193E-5	2.9437352114102317E-5	0.0	0.0825325228772382	5.789345915773455E-4
29	2.453112676175193E-5	2.9437352114102317E-5	0.0	0.15909416950066596	5.936532676343967E-4
30	2.453112676175193E-5	2.9437352114102317E-5	0.0	0.2587445126322547	5.936532676343967E-4
31	2.453112676175193E-5	2.9437352114102317E-5	0.0	0.5586571621960814	5.985594929867472E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17945	0.0	19.618557	1
CTTATAC	13660	0.0	15.845534	37
TCTTATA	21945	0.0	14.457735	37
GTATCAA	25800	0.0	13.6598835	2
TCGTTAC	2500	0.0	12.432	23
CTCTTAT	33190	0.0	12.335191	37
TATACCG	695	0.0	11.446043	5
CTCGTTA	2955	0.0	11.018612	22
AACGCAG	32075	0.0	10.889478	7
TATACTG	2865	0.0	10.783596	5
CGTTACG	2120	0.0	10.733491	24
GTCTTAG	2930	0.0	10.418089	1
TCAACGC	33930	0.0	10.414088	5
ATCAACG	34205	0.0	10.37363	4
TCTATAC	1950	0.0	10.341025	3
TATCAAC	34745	0.0	10.32422	3
TCTCTTA	46195	0.0	10.316268	37
CAACGCA	34455	0.0	10.250036	6
TACTTAC	4165	0.0	10.216086	31
TAGATCG	785	0.0	10.133758	5
>>END_MODULE