##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727132.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10069145
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.485725451366527	31.0	31.0	34.0	30.0	34.0
2	31.728257960333277	31.0	31.0	34.0	30.0	34.0
3	31.826130421202595	31.0	31.0	34.0	30.0	34.0
4	35.55664239615181	37.0	35.0	37.0	33.0	37.0
5	35.09601113103446	37.0	35.0	37.0	32.0	37.0
6	35.109562728513694	37.0	35.0	37.0	32.0	37.0
7	35.072495926913355	37.0	35.0	37.0	32.0	37.0
8	35.02930884399817	37.0	35.0	37.0	32.0	37.0
9	36.77530316625692	39.0	37.0	39.0	33.0	39.0
10	36.53245881353382	38.0	35.0	39.0	32.0	39.0
11	36.6368480144044	38.0	35.0	39.0	32.0	39.0
12	36.5202305657531	38.0	35.0	39.0	32.0	39.0
13	36.61832578635028	38.0	35.0	39.0	32.0	39.0
14	37.67751591619745	39.0	37.0	40.0	33.0	41.0
15	37.6043964010847	39.0	37.0	40.0	32.0	41.0
16	37.55784974791802	39.0	37.0	40.0	32.0	41.0
17	37.49172129311873	39.0	36.0	40.0	32.0	41.0
18	37.48382111887355	39.0	36.0	40.0	32.0	41.0
19	37.52865402176649	39.0	37.0	40.0	32.0	41.0
20	37.431543889774154	39.0	36.0	40.0	32.0	41.0
21	37.3593845356284	39.0	36.0	40.0	32.0	41.0
22	37.28335335323903	39.0	36.0	40.0	32.0	41.0
23	37.215590996057756	39.0	36.0	40.0	31.0	41.0
24	37.112304570050384	39.0	36.0	40.0	31.0	41.0
25	37.072603383902006	39.0	36.0	40.0	31.0	41.0
26	36.87176766249766	39.0	36.0	40.0	31.0	41.0
27	36.7095179382162	39.0	36.0	40.0	30.0	41.0
28	36.609751374123626	39.0	35.0	40.0	30.0	41.0
29	36.49666669811588	38.0	35.0	40.0	30.0	41.0
30	36.4335714700702	38.0	35.0	40.0	30.0	41.0
31	36.39144366279361	38.0	35.0	40.0	30.0	41.0
32	36.273514782039584	38.0	35.0	40.0	30.0	41.0
33	36.184473557586074	38.0	35.0	40.0	30.0	41.0
34	36.141431273459666	38.0	35.0	40.0	30.0	41.0
35	36.05265452031925	38.0	35.0	40.0	29.0	41.0
36	35.91973300612912	38.0	35.0	40.0	29.0	41.0
37	35.828001384427374	38.0	35.0	40.0	28.0	41.0
38	35.78311326334063	38.0	35.0	40.0	28.0	41.0
39	35.67590475656076	38.0	34.0	40.0	27.0	41.0
40	35.57977385368867	38.0	34.0	40.0	27.0	41.0
41	35.52956621441046	38.0	34.0	40.0	27.0	41.0
42	35.38114050398519	38.0	34.0	40.0	27.0	41.0
43	34.071847411076114	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	12.0
10	18.0
11	12.0
12	12.0
13	18.0
14	30.0
15	44.0
16	140.0
17	309.0
18	658.0
19	1353.0
20	2867.0
21	5855.0
22	10826.0
23	18546.0
24	30439.0
25	47601.0
26	70417.0
27	101731.0
28	140861.0
29	189364.0
30	246966.0
31	317487.0
32	403483.0
33	512754.0
34	662430.0
35	879479.0
36	1215382.0
37	1751618.0
38	2401221.0
39	1057212.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.09033636917533	18.992933362266605	12.985849344706029	25.930880923852023
2	18.876270030871538	20.90875640384561	35.36215835604711	24.85281520923574
3	20.365562319342903	22.56022730827692	29.556859097768477	27.517351274611695
4	15.598424692463958	15.956180986568372	33.56632564135287	34.879068679614804
5	15.779740980986965	35.26420565003285	33.60021133869857	15.355842030281618
6	35.577608625161325	34.01485429001171	15.01891173480966	15.388625350017303
7	29.384639907360555	29.793890146581465	21.22585383366711	19.595616112390875
8	26.018296488927312	35.01949768326904	19.49751443642931	19.46469139137434
9	26.250034138946255	15.049083114802697	19.688831574081018	39.01205117217003
10	17.539572625083856	26.364214637886334	32.90495866332246	23.191254073707352
11	35.68680359653178	21.71615365554871	21.298322747363358	21.298720000556155
12	21.484127996964986	24.803784233914598	30.085046942913223	23.627040826207192
13	30.850782266021593	19.719757735140373	24.163551125741066	25.265908873096972
14	22.33761655036252	20.640153657534974	25.362729407511758	31.65950038459075
15	25.110642462691718	27.442627949046315	22.44119038905488	25.00553919920708
16	24.655062569860696	25.985205297967205	24.502437893187555	24.857294238984544
17	24.06758468569079	26.28612459151199	25.472778473246738	24.173512249550484
18	24.277900457288084	25.260347328397792	26.098839573767187	24.362912640546938
19	25.00396011776571	25.21545771761157	26.31539222049141	23.465189944131303
20	24.63260783313777	25.19153314407529	26.19679227978145	23.97906674300549
21	24.391455282449503	25.29352790132628	26.23415394256414	24.080862873660077
22	24.779293574578574	25.116343046008378	25.821368149927327	24.28299522948572
23	24.32329656589512	25.212915297177663	25.8041372926897	24.659650844237518
24	24.357192194570644	25.302426372844966	25.839323994241813	24.50105743834258
25	24.733410830810364	25.030933609556723	25.753626549225384	24.482029010407537
26	24.34713175746302	25.359114403457294	25.96951379685167	24.324240042228016
27	24.54331524672651	25.50148001642642	25.752325545018966	24.202879191828107
28	24.39716579709598	25.41734179019172	25.92057220349891	24.264920209213393
29	24.26613183145143	25.62741920987333	26.08291965206579	24.023529306609447
30	24.311945055910904	25.575994784065582	25.954795566058486	24.157264593965028
31	24.444915630870348	25.018996151113125	26.083267248609488	24.452820969407036
32	23.968380632119214	25.44024343675655	26.059700202946722	24.531675728177515
33	24.138156715391425	25.04334777183167	26.040920058257182	24.777575454519724
34	24.345999585863545	24.88002705294243	26.341730107173944	24.43224325402008
35	24.191428368545694	24.913316870498935	26.392300438617184	24.50295432233819
36	23.98332728349825	24.604194298522863	26.65939362279518	24.753084795183703
37	23.887688577332035	24.162945314622046	27.039157743780628	24.910208364265287
38	23.57355068379689	24.235861138160192	27.50377514674781	24.68681303129511
39	23.390486481225565	24.0999310269144	27.849196729215837	24.660385762644196
40	23.395670635391586	24.095551310463797	27.969028154823473	24.539749899321144
41	22.962754037209713	24.11196779865619	28.154098486018427	24.77117967811567
42	22.652191422409746	24.400403410617287	28.048260304127115	24.89914486284585
43	22.622079630395632	24.15999570966552	28.024971335699306	25.192953324239546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8186.0
1	6433.5
2	4681.0
3	7732.5
4	10784.0
5	10784.0
6	12784.5
7	14785.0
8	13393.5
9	12002.0
10	16325.0
11	20648.0
12	20648.0
13	34989.5
14	49331.0
15	60552.0
16	71773.0
17	65884.0
18	59995.0
19	59995.0
20	69840.0
21	79685.0
22	71055.5
23	62426.0
24	73696.0
25	84966.0
26	84966.0
27	103556.0
28	122146.0
29	140907.0
30	159668.0
31	185838.5
32	212009.0
33	212009.0
34	247026.5
35	282044.0
36	324198.0
37	366352.0
38	415531.5
39	464711.0
40	464711.0
41	513904.5
42	563098.0
43	616460.5
44	669823.0
45	715910.0
46	761997.0
47	761997.0
48	805829.5
49	849662.0
50	868398.0
51	887134.0
52	880363.0
53	873592.0
54	873592.0
55	838242.5
56	802893.0
57	751183.0
58	699473.0
59	638330.5
60	577188.0
61	577188.0
62	506293.0
63	435398.0
64	371142.5
65	306887.0
66	256473.0
67	206059.0
68	206059.0
69	169625.0
70	133191.0
71	110035.0
72	86879.0
73	70622.0
74	54365.0
75	54365.0
76	43825.5
77	33286.0
78	26586.0
79	19886.0
80	15287.5
81	10689.0
82	10689.0
83	8274.0
84	5859.0
85	4657.5
86	3456.0
87	2436.0
88	1416.0
89	1416.0
90	957.0
91	498.0
92	316.0
93	134.0
94	102.5
95	71.0
96	71.0
97	42.5
98	14.0
99	9.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.0069145E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.55087943586181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.43650156805437	41.74458016709473
2	10.29694528278527	10.822270602266295
3	3.639061105925132	5.737075843116168
4	1.8194402272782653	3.8245273609782844
5	1.0570472107711175	2.7774380265623497
6	0.719585074270413	2.268889708909809
7	0.48270585391506254	1.7756631992452623
8	0.3605653972466877	1.5158422975563444
9	0.27653699624408795	1.3079036114260594
>10	1.722903846982847	17.05312320899763
>50	0.13517328045456012	4.861420502643233
>100	0.052057949167503746	4.547034389484507
>500	0.0011359549769553504	0.38517595212075634
>1k	2.0793187178265316E-4	0.2584233795896065
>5k	5.670859540230913E-5	0.17494101469174247
>10k+	7.561146053641218E-5	0.9456907353170504
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31039	0.308258546281735	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27798	0.27607110633524495	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20669	0.20527065604875094	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15695	0.15587222152427044	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.931329819959887E-6	3.972531927983955E-5
2	0.0	9.931329819959887E-6	0.0	9.931329819959887E-6	4.965664909979944E-5
3	0.0	9.931329819959887E-6	0.0	9.931329819959887E-6	4.965664909979944E-5
4	0.0	9.931329819959887E-6	0.0	9.931329819959887E-6	7.94506385596791E-5
5	0.0	9.931329819959887E-6	0.0	9.931329819959887E-6	7.94506385596791E-5
6	9.931329819959887E-6	9.931329819959887E-6	0.0	9.931329819959887E-6	9.931329819959887E-5
7	9.931329819959887E-6	9.931329819959887E-6	0.0	9.931329819959887E-6	9.931329819959887E-5
8	9.931329819959887E-6	9.931329819959887E-6	0.0	9.931329819959887E-6	1.0924462801955876E-4
9	9.931329819959887E-6	9.931329819959887E-6	0.0	1.9862659639919775E-5	1.2910728765947854E-4
10	1.9862659639919775E-5	9.931329819959887E-6	0.0	2.9793989459879662E-5	1.2910728765947854E-4
11	2.9793989459879662E-5	9.931329819959887E-6	0.0	2.9793989459879662E-5	1.2910728765947854E-4
12	2.9793989459879662E-5	9.931329819959887E-6	0.0	3.972531927983955E-5	1.3903861747943842E-4
13	2.9793989459879662E-5	9.931329819959887E-6	0.0	4.965664909979944E-5	1.3903861747943842E-4
14	4.965664909979944E-5	9.931329819959887E-6	0.0	6.951930873971921E-5	2.1848925603911752E-4
15	4.965664909979944E-5	1.9862659639919775E-5	0.0	7.94506385596791E-5	2.5821457531895707E-4
16	5.9587978919759324E-5	1.9862659639919775E-5	0.0	1.3903861747943842E-4	2.979398945987966E-4
17	5.9587978919759324E-5	1.9862659639919775E-5	0.0	1.8869526657923786E-4	2.979398945987966E-4
18	5.9587978919759324E-5	1.9862659639919775E-5	0.0	2.6814590513891696E-4	3.3766521387863617E-4
19	5.9587978919759324E-5	1.9862659639919775E-5	0.0	3.6745920333851583E-4	3.3766521387863617E-4
20	5.9587978919759324E-5	1.9862659639919775E-5	0.0	4.866351611780345E-4	3.3766521387863617E-4
21	5.9587978919759324E-5	1.9862659639919775E-5	0.0	8.342317048766305E-4	3.6745920333851583E-4
22	5.9587978919759324E-5	1.9862659639919775E-5	0.0	0.001241416227494986	3.6745920333851583E-4
23	5.9587978919759324E-5	1.9862659639919775E-5	0.0	0.0017876393675927797	4.4690984189819493E-4
24	5.9587978919759324E-5	1.9862659639919775E-5	0.0	0.003098574903827485	4.568411717181548E-4
25	5.9587978919759324E-5	1.9862659639919775E-5	0.0	0.004886214271420265	4.667725015381147E-4
26	5.9587978919759324E-5	1.9862659639919775E-5	0.0	0.008630325613545143	4.667725015381147E-4
27	5.9587978919759324E-5	1.9862659639919775E-5	0.0	0.032455585851628915	4.866351611780345E-4
28	5.9587978919759324E-5	1.9862659639919775E-5	0.0	0.09685032840424883	4.965664909979944E-4
29	5.9587978919759324E-5	1.9862659639919775E-5	0.0	0.1825974300697825	4.965664909979944E-4
30	5.9587978919759324E-5	1.9862659639919775E-5	0.0	0.29186192074898115	5.064978208179543E-4
31	5.9587978919759324E-5	1.9862659639919775E-5	0.0	0.5978064671826655	5.064978208179543E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13690	0.0	16.418919	1
CTTATAC	7135	0.0	15.97197	37
TCTTATA	11435	0.0	14.010493	37
CGTATAC	260	1.8189894E-12	13.519231	3
CTCTTAT	17640	0.0	12.836735	37
GTATTAG	1200	0.0	12.025	1
GTATCAA	18805	0.0	11.943101	2
GTCTTAG	1435	0.0	11.602787	1
TCTCTTA	24890	0.0	10.82945	37
TATACCG	480	0.0	10.791667	5
AACGCAG	21200	0.0	10.340802	7
GTATTAT	1090	0.0	10.183486	1
GTACTAG	820	0.0	10.15244	1
TAGTACT	830	0.0	10.030121	4
ACCGTCC	980	0.0	10.005102	8
TAGGACT	1390	0.0	9.982015	4
CGTACAC	600	0.0	9.866666	3
CTAGAGT	1820	0.0	9.758243	4
GTACCGT	515	0.0	9.69903	6
CTAGACA	1300	0.0	9.676924	4
>>END_MODULE