FastQCFastQC Report
Thu 9 Feb 2017
SRR2727131.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727131.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908493
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78520.4114240922025913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT76410.40036824866530824No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46540.24385732617305905No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40480.21210452435508018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT555.144805E-420.1818184
GGTATCA37650.015.920321
CTTATAC8650.014.97109837
TCTATTC1651.646913E-814.5757573
TCTTATA14500.014.16206937
ATACCGT800.006300630513.8750016
CTATACT1951.0270014E-813.2820524
TCGAACG850.00940770713.0588233
CAATACG1000.001833251412.9500014
ATTATAC2602.3646862E-1112.8076933
GTCTTAT1755.309503E-712.6857151
CTCTTAT22300.012.60986537
GGGTTAT1951.4686884E-712.3333341
CATATAG1050.002644672512.3333331
GTATAAC2102.8936483E-812.3333333
GTATCAA49500.012.1090912
ACCGTGC2002.041561E-712.0250018
GTAATAC1851.0401745E-612.03
GACCCGT1100.003746602511.7727276
CGTGCGC1951.9617528E-611.38461610
Produced by FastQC (version 0.11.5)