##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727131.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1908493
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.779754497396638	31.0	30.0	31.0	26.0	34.0
2	30.032700146136246	31.0	30.0	31.0	26.0	34.0
3	30.0875549451845	31.0	30.0	31.0	26.0	34.0
4	33.701409436660235	35.0	33.0	37.0	28.0	37.0
5	31.990965122743443	35.0	30.0	37.0	22.0	37.0
6	32.22176450214908	35.0	32.0	36.0	25.0	37.0
7	32.236775298625666	35.0	32.0	35.0	25.0	37.0
8	32.14364003430979	35.0	32.0	35.0	25.0	37.0
9	33.46375229041972	35.0	32.0	38.0	25.0	39.0
10	32.917043971342835	35.0	31.0	38.0	23.0	39.0
11	33.29824578869297	35.0	32.0	38.0	25.0	39.0
12	33.00325544814678	35.0	31.0	38.0	25.0	39.0
13	33.28459365583211	35.0	32.0	38.0	25.0	39.0
14	33.80987459739176	36.0	32.0	39.0	25.0	40.0
15	33.783965673439724	36.0	32.0	39.0	25.0	40.0
16	33.665924370694576	36.0	31.0	39.0	25.0	40.0
17	33.57676030250045	36.0	31.0	39.0	24.0	40.0
18	33.5218730170873	36.0	31.0	39.0	24.0	40.0
19	33.561923465268144	36.0	31.0	39.0	24.0	40.0
20	33.40312225405071	36.0	31.0	39.0	24.0	40.0
21	33.279233405624225	36.0	31.0	39.0	23.0	40.0
22	33.17940857000785	36.0	31.0	39.0	23.0	40.0
23	33.044495316461735	36.0	30.0	39.0	22.0	40.0
24	32.86377209662283	36.0	30.0	39.0	21.0	40.0
25	32.79748262110471	36.0	30.0	38.0	21.0	40.0
26	32.43878442310242	35.0	30.0	38.0	18.0	40.0
27	32.18803160399331	35.0	30.0	38.0	18.0	40.0
28	32.002205928971186	35.0	29.0	38.0	18.0	40.0
29	31.80748789751914	35.0	29.0	38.0	17.0	40.0
30	31.67082038026862	35.0	28.0	38.0	17.0	40.0
31	31.56777991850114	34.0	28.0	38.0	16.0	40.0
32	31.37967129038461	34.0	27.0	38.0	16.0	40.0
33	31.23159843918736	34.0	27.0	38.0	16.0	40.0
34	31.1282959906062	34.0	27.0	38.0	16.0	40.0
35	30.973006450639325	34.0	27.0	38.0	15.0	40.0
36	30.734367901794766	34.0	26.0	38.0	15.0	40.0
37	30.59814733404838	34.0	26.0	38.0	15.0	40.0
38	30.483278691616892	34.0	26.0	38.0	15.0	40.0
39	30.315862306018413	33.0	25.0	38.0	14.0	40.0
40	30.16726338529929	33.0	25.0	38.0	13.0	40.0
41	30.040141095618374	33.0	25.0	38.0	13.0	40.0
42	29.784600205502457	33.0	25.0	38.0	12.0	40.0
43	28.525940624356494	32.0	23.0	37.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	15.0
11	38.0
12	64.0
13	181.0
14	388.0
15	803.0
16	1605.0
17	2952.0
18	5050.0
19	8317.0
20	12645.0
21	18883.0
22	26330.0
23	36143.0
24	47686.0
25	59950.0
26	74493.0
27	88428.0
28	102411.0
29	117274.0
30	129409.0
31	140779.0
32	147676.0
33	153039.0
34	153127.0
35	149396.0
36	140374.0
37	130208.0
38	115260.0
39	45566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.66302208077263	22.12756347547515	14.35368115052033	23.855733293231886
2	20.889570986113128	23.77404580472656	32.72644961233811	22.609933596822206
3	22.42512809845255	24.32091707960155	28.974012480003857	24.279942341942046
4	18.567267472293583	17.604151547844292	32.144314912341834	31.68426606752029
5	15.813000100078964	36.829477498738534	32.33415055753414	15.023371843648365
6	34.862008925366766	35.32080023348264	14.39512746444446	15.422063376706124
7	28.85035470394704	30.808548944114545	21.0553038444469	19.285792507491514
8	26.23604068760011	35.89580889214684	19.00017448321791	18.867975937035137
9	26.548328969506308	15.307103562863475	19.54536904248535	38.59919842514486
10	17.742952161731797	27.679221249436075	32.33168788148555	22.24613870734658
11	35.82313375003209	22.30021278569007	20.933741962899525	20.942911501378312
12	21.391223336947004	25.849976918961715	29.782398992293917	22.97640075179736
13	31.28761803160923	20.25629646008657	24.198883621789548	24.257201886514647
14	22.29560181776931	21.486691331851883	25.733287992148778	30.48441885823003
15	25.361790690350972	28.167669464860495	22.47108058557197	23.999459259216565
16	24.368965461230406	26.598787629821015	24.84190405728499	24.19034285166359
17	24.076692971889337	26.96892260018769	25.508974882276224	23.445409545646747
18	24.18945209649708	25.714529736289315	26.349952554188043	23.746065613025564
19	25.13286661255766	25.66297073135715	26.68760115965843	22.516561496426764
20	24.591759047583615	25.593387033643822	26.61089142061302	23.20396249815954
21	24.273811850501943	25.765197986054964	26.667742559181512	23.293247604261584
22	24.66385781870827	25.657783392446294	26.24327152365767	23.435087265187768
23	24.244050148467927	25.69755299076287	26.23462595880624	23.823770901962963
24	24.30839410990766	25.7941213302852	26.1567110804179	23.740773479389237
25	24.658722877160148	25.50294918556159	26.21041837722224	23.627909560056022
26	24.294351616694428	25.8315854446414	26.333447384926224	23.540615553737947
27	24.567027492372254	25.943768198259043	26.13271308828484	23.35649122108386
28	24.447299518520634	25.901588321256614	26.237507813756718	23.413604346466034
29	24.283557759970826	26.122443205188596	26.398315319993316	23.195683714847267
30	24.342871574587907	26.025927263028997	26.234311574629825	23.396889587753268
31	24.425973791887106	25.502530006659708	26.492211394016117	23.579284807437073
32	23.992280820521742	25.92202329272363	26.296297654746443	23.789398232008185
33	24.192229156722085	25.612774057856118	26.26957499975111	23.925421785670682
34	24.470616344938126	25.50687898776679	26.478273695528358	23.54423097176673
35	24.310332812328888	25.553879422140923	26.434155116104698	23.701632649425488
36	24.14727221949465	25.316833753123536	26.55540261347566	23.980491413906154
37	24.103048845345516	25.057571602306112	26.938584527163577	23.90079502518479
38	23.896079262538557	25.03210648401645	27.147911991293654	23.92390226215134
39	23.701370662611808	25.053379813287236	27.389568628231803	23.85568089586915
40	23.851489106850273	24.931870329102594	27.475971879383366	23.740668684663763
41	23.557015928274296	24.98369132084844	27.454698550112578	24.004594200764686
42	23.360159036475377	25.144132045545888	27.332507900212367	24.163201017766372
43	23.667102787382504	24.861972247212854	27.038296708450073	24.432628256954573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2436.0
1	1924.0
2	1412.0
3	1835.0
4	2258.0
5	2258.0
6	2822.5
7	3387.0
8	3291.0
9	3195.0
10	4192.5
11	5190.0
12	5190.0
13	9015.0
14	12840.0
15	16576.0
16	20312.0
17	18122.5
18	15933.0
19	15933.0
20	17992.0
21	20051.0
22	17350.5
23	14650.0
24	16283.5
25	17917.0
26	17917.0
27	21152.5
28	24388.0
29	28155.5
30	31923.0
31	37137.0
32	42351.0
33	42351.0
34	48524.5
35	54698.0
36	62803.5
37	70909.0
38	79624.5
39	88340.0
40	88340.0
41	97457.5
42	106575.0
43	116334.0
44	126093.0
45	134577.5
46	143062.0
47	143062.0
48	150883.0
49	158704.0
50	162747.5
51	166791.0
52	164528.0
53	162265.0
54	162265.0
55	154355.5
56	146446.0
57	136512.5
58	126579.0
59	115341.5
60	104104.0
61	104104.0
62	91674.0
63	79244.0
64	67329.5
65	55415.0
66	46530.5
67	37646.0
68	37646.0
69	31194.0
70	24742.0
71	20293.5
72	15845.0
73	12834.5
74	9824.0
75	9824.0
76	7789.5
77	5755.0
78	4585.5
79	3416.0
80	2629.5
81	1843.0
82	1843.0
83	1439.5
84	1036.0
85	788.5
86	541.0
87	391.0
88	241.0
89	241.0
90	170.0
91	99.0
92	60.5
93	22.0
94	16.5
95	11.0
96	11.0
97	7.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1908493.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.17048262577845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.39859604849165	65.56566532357857
2	6.4483608184397605	9.565560680976736
3	2.0543659952093765	4.571199520640013
4	0.9772772328537703	2.899404960797976
5	0.5642575075492676	2.0925625830073997
6	0.36631853014365084	1.630201330531221
7	0.23962445692640716	1.2441143133420174
8	0.1770430390740078	1.0505094122922967
9	0.1482965181291961	0.9899301889228477
>10	0.6052253676926614	7.3347400144550186
>50	0.01564102218883323	0.777656744986701
>100	0.004137948699271317	0.5245882753740434
>500	2.1387865054426313E-4	0.11828870292148674
>1k	4.990501846032751E-4	0.8163348215028599
>5k	1.4258576702950716E-4	0.8192431266707998
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7852	0.4114240922025913	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7641	0.40036824866530824	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4654	0.24385732617305905	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4048	0.21210452435508018	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	5.239736273593877E-5	0.0
12	0.0	0.0	0.0	5.239736273593877E-5	0.0
13	0.0	0.0	0.0	5.239736273593877E-5	5.239736273593877E-5
14	0.0	0.0	5.239736273593877E-5	1.0479472547187755E-4	1.0479472547187755E-4
15	0.0	5.239736273593877E-5	5.239736273593877E-5	1.0479472547187755E-4	1.0479472547187755E-4
16	0.0	5.239736273593877E-5	5.239736273593877E-5	1.5719208820781633E-4	1.0479472547187755E-4
17	0.0	5.239736273593877E-5	5.239736273593877E-5	2.6198681367969385E-4	1.0479472547187755E-4
18	0.0	5.239736273593877E-5	5.239736273593877E-5	2.6198681367969385E-4	1.0479472547187755E-4
19	0.0	5.239736273593877E-5	5.239736273593877E-5	4.191789018875102E-4	1.0479472547187755E-4
20	0.0	5.239736273593877E-5	5.239736273593877E-5	4.71576264623449E-4	1.0479472547187755E-4
21	0.0	5.239736273593877E-5	5.239736273593877E-5	8.907551665109591E-4	1.0479472547187755E-4
22	5.239736273593877E-5	5.239736273593877E-5	5.239736273593877E-5	0.0013623314311344082	1.0479472547187755E-4
23	5.239736273593877E-5	5.239736273593877E-5	5.239736273593877E-5	0.0016767156075500407	1.5719208820781633E-4
24	5.239736273593877E-5	5.239736273593877E-5	5.239736273593877E-5	0.0022530865976453673	1.5719208820781633E-4
25	5.239736273593877E-5	5.239736273593877E-5	5.239736273593877E-5	0.003248636489628204	1.5719208820781633E-4
26	5.239736273593877E-5	5.239736273593877E-5	5.239736273593877E-5	0.005501723087273571	1.5719208820781633E-4
27	5.239736273593877E-5	5.239736273593877E-5	5.239736273593877E-5	0.017238732340123856	1.5719208820781633E-4
28	5.239736273593877E-5	5.239736273593877E-5	5.239736273593877E-5	0.05653675439207794	1.5719208820781633E-4
29	5.239736273593877E-5	5.239736273593877E-5	5.239736273593877E-5	0.11417385340161058	1.5719208820781633E-4
30	5.239736273593877E-5	5.239736273593877E-5	5.239736273593877E-5	0.18805413485928427	1.5719208820781633E-4
31	5.239736273593877E-5	5.239736273593877E-5	5.239736273593877E-5	0.39056994183368765	2.6198681367969385E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	55	5.144805E-4	20.181818	4
GGTATCA	3765	0.0	15.92032	1
CTTATAC	865	0.0	14.971098	37
TCTATTC	165	1.646913E-8	14.575757	3
TCTTATA	1450	0.0	14.162069	37
ATACCGT	80	0.0063006305	13.875001	6
CTATACT	195	1.0270014E-8	13.282052	4
TCGAACG	85	0.009407707	13.058823	3
CAATACG	100	0.0018332514	12.950001	4
ATTATAC	260	2.3646862E-11	12.807693	3
GTCTTAT	175	5.309503E-7	12.685715	1
CTCTTAT	2230	0.0	12.609865	37
GGGTTAT	195	1.4686884E-7	12.333334	1
CATATAG	105	0.0026446725	12.333333	1
GTATAAC	210	2.8936483E-8	12.333333	3
GTATCAA	4950	0.0	12.109091	2
ACCGTGC	200	2.041561E-7	12.025001	8
GTAATAC	185	1.0401745E-6	12.0	3
GACCCGT	110	0.0037466025	11.772727	6
CGTGCGC	195	1.9617528E-6	11.384616	10
>>END_MODULE