FastQCFastQC Report
Thu 9 Feb 2017
SRR2727130.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727130.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2148934
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT96180.44757074903184557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94930.44175391147424725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71720.3337468717047615No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44260.2059625842394415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15300.016.3235337
CGTAACC700.002593477515.8571433
GGTATCA47750.015.3811511
TATACCG1152.2115026E-514.4782615
TACCGTC908.2781457E-414.3888887
TCTTATA24950.013.64328737
AATAGCG1000.00183338212.9500015
TATACTG3600.012.8472225
CGGTCGT2451.1459633E-1012.83673617
GTCGTGT1602.6979033E-612.718751
CTCTTAT38050.012.3495437
ACTATAC1807.4703894E-712.3333333
TGGACCG2102.894376E-812.3333335
ATAGCGT1205.124664E-412.3333336
GTATTAC2154.0168743E-812.0465121
TACTAAG1705.3139993E-611.9705882
GTGCTAT1552.7222655E-511.9354831
GTCTAGA2808.367351E-1111.8928581
GATAGTA1401.3993902E-411.8928582
TACCGGC1401.3993902E-411.8928587
Produced by FastQC (version 0.11.5)