##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727130.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2148934
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.534585520076465	31.0	31.0	34.0	30.0	34.0
2	31.787143299887294	31.0	31.0	34.0	30.0	34.0
3	31.899064373312534	31.0	31.0	34.0	30.0	34.0
4	35.63935793281692	37.0	35.0	37.0	33.0	37.0
5	35.22276998735187	37.0	35.0	37.0	32.0	37.0
6	35.236758783657386	37.0	35.0	37.0	32.0	37.0
7	35.18932456743669	37.0	35.0	37.0	32.0	37.0
8	35.14258278755886	37.0	35.0	37.0	32.0	37.0
9	36.96880360215809	39.0	37.0	39.0	33.0	39.0
10	36.72854354763804	39.0	35.0	39.0	32.0	39.0
11	36.85457161550797	39.0	37.0	39.0	33.0	39.0
12	36.722805819071226	39.0	35.0	39.0	32.0	39.0
13	36.86313353504575	39.0	37.0	39.0	33.0	39.0
14	37.869906660697815	39.0	37.0	41.0	33.0	41.0
15	37.874306051279376	39.0	37.0	41.0	33.0	41.0
16	37.809778243538425	39.0	37.0	41.0	33.0	41.0
17	37.73073672807075	39.0	37.0	41.0	33.0	41.0
18	37.75332374098041	39.0	37.0	41.0	33.0	41.0
19	37.80324337555272	39.0	37.0	41.0	33.0	41.0
20	37.676707614100756	39.0	37.0	41.0	32.0	41.0
21	37.66359087808188	39.0	37.0	40.0	32.0	41.0
22	37.5655227196368	39.0	37.0	40.0	32.0	41.0
23	37.50442219258479	39.0	36.0	40.0	32.0	41.0
24	37.390370295225445	39.0	36.0	40.0	32.0	41.0
25	37.36506705184989	39.0	36.0	40.0	32.0	41.0
26	37.210813826762475	39.0	36.0	40.0	31.0	41.0
27	37.01857572172994	39.0	36.0	40.0	31.0	41.0
28	36.90230784193465	39.0	36.0	40.0	31.0	41.0
29	36.81039575901354	39.0	36.0	40.0	30.0	41.0
30	36.74781403244585	39.0	35.0	40.0	30.0	41.0
31	36.69696277317033	39.0	35.0	40.0	30.0	41.0
32	36.58281920245108	38.0	35.0	40.0	30.0	41.0
33	36.54242801314512	38.0	35.0	40.0	30.0	41.0
34	36.515958144829014	38.0	35.0	40.0	30.0	41.0
35	36.412282554978425	38.0	35.0	40.0	30.0	41.0
36	36.31821638077298	38.0	35.0	40.0	30.0	41.0
37	36.16970507237542	38.0	35.0	40.0	30.0	41.0
38	36.10348619361972	38.0	35.0	40.0	29.0	41.0
39	35.985768757905085	38.0	35.0	40.0	29.0	41.0
40	35.90773658009041	38.0	35.0	40.0	29.0	41.0
41	35.834651506281716	38.0	35.0	40.0	28.0	41.0
42	35.763446434371645	38.0	35.0	40.0	28.0	41.0
43	34.41439011156229	37.0	33.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	8.0
12	5.0
13	3.0
14	2.0
15	5.0
16	6.0
17	32.0
18	79.0
19	138.0
20	328.0
21	730.0
22	1333.0
23	2546.0
24	4455.0
25	7338.0
26	11588.0
27	17450.0
28	24994.0
29	35127.0
30	48126.0
31	63130.0
32	82670.0
33	106730.0
34	139443.0
35	184413.0
36	250678.0
37	351020.0
38	503493.0
39	313058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64900643761046	18.5983841290612	12.891508068651714	25.861101364676625
2	18.66883766555883	21.00031922804516	35.42007339452957	24.910769711866443
3	20.15087480583396	22.64234266850448	30.039638257852495	27.167144267809064
4	15.209494568004414	15.684427720907204	33.70280334342516	35.40327436766322
5	15.699737637358801	35.42858924471389	33.496515016282494	15.375158101644814
6	35.296477230105715	33.82114108669694	15.10809545570036	15.774286227496981
7	29.089260070341854	30.309306847022754	21.25947097491128	19.341962107724108
8	26.499464385597697	34.59022008121236	19.60632574104184	19.30398979214811
9	26.828232044353157	14.743403008189176	19.68329413560398	38.745070811853694
10	17.498396879569125	26.4524643381323	32.65795971397912	23.391179068319456
11	35.49480812346959	21.86353792159275	21.304470030256862	21.337183924680794
12	21.688520913159735	24.976569778317995	29.92967676066366	23.405232547858613
13	31.028593712045133	19.78525166431356	24.353284000346218	24.832870623295083
14	22.246378902283645	20.88365673398997	25.956590570022158	30.91337379370423
15	25.391147424723144	27.353981090159117	22.880786473665548	24.37408501145219
16	24.17128678684408	26.172092767856064	25.05605104670502	24.60056939859484
17	24.04289754827277	26.13458579928467	25.59552782914692	24.226988823295642
18	24.02386485578431	25.10407485758055	26.5825288259202	24.28953146071494
19	24.984387607995405	25.090347120944617	26.97946982085071	22.945795450209268
20	24.35919390730474	24.900299404262764	26.819250847164223	23.921255841268273
21	23.929725156752138	25.479377216796795	27.23480572227905	23.356091904172022
22	24.58656245375614	25.031015377857113	26.21876707241823	24.16365509596851
23	23.898267699240648	25.19281653135927	26.698121021864797	24.21079474753529
24	23.821997325185418	25.271553244538918	26.635578384445495	24.270871045830162
25	24.225453178180437	25.003699508686633	26.49374061744102	24.27710669569191
26	23.749728935369816	25.323113692649475	26.78588546693384	24.141271905046874
27	24.26528688177487	25.747556695552305	26.329985006519514	23.65717141615331
28	23.915252864908833	25.716657654446344	26.284101791865176	24.083987688779647
29	23.76057152057718	25.90279645628949	26.68727843665743	23.6493535864759
30	24.114281778779617	25.68682891145098	26.426730648777486	23.772158660991916
31	23.9936173004848	25.0503738132488	26.78481516882324	24.17119371744316
32	23.397228579379355	25.786878517441668	26.539530762694437	24.27636214048454
33	23.6187337535727	25.104447135184238	26.73874581536706	24.538073295876
34	23.939637047950285	25.15442540347912	27.11372243168008	23.792215116890514
35	23.789329965461945	25.229346271220987	26.88761032214112	24.09371344117595
36	23.45320982403368	24.86763204453929	27.30781866730202	24.371339464125004
37	23.29024530302001	24.63672686085287	27.55049713020502	24.5225307059221
38	23.240871985831113	24.413406833341554	27.90104302877613	24.444678152051203
39	22.851562681776176	24.431834574724025	28.449919820711106	24.266682922788693
40	23.226073951084583	24.212842274355566	28.480539653614308	24.08054412094555
41	22.56756140486399	24.3982830556918	28.617444742369933	24.41671079707427
42	22.18113725223762	24.65920312117543	28.55145853711654	24.60820108947041
43	21.957817224726305	24.27836313260435	28.47667727347606	25.287142369193283
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1223.0
1	1072.5
2	922.0
3	1818.0
4	2714.0
5	2714.0
6	3296.0
7	3878.0
8	3664.0
9	3450.0
10	5003.0
11	6556.0
12	6556.0
13	10331.0
14	14106.0
15	18280.0
16	22454.0
17	20426.0
18	18398.0
19	18398.0
20	20426.5
21	22455.0
22	18996.5
23	15538.0
24	18064.5
25	20591.0
26	20591.0
27	24371.5
28	28152.0
29	32035.5
30	35919.0
31	40820.5
32	45722.0
33	45722.0
34	52465.0
35	59208.0
36	66964.5
37	74721.0
38	84351.5
39	93982.0
40	93982.0
41	104444.5
42	114907.0
43	126411.5
44	137916.0
45	149344.5
46	160773.0
47	160773.0
48	171794.5
49	182816.0
50	187260.0
51	191704.0
52	189656.5
53	187609.0
54	187609.0
55	177667.0
56	167725.0
57	157436.5
58	147148.0
59	134029.5
60	120911.0
61	120911.0
62	105936.5
63	90962.0
64	77005.0
65	63048.0
66	52160.0
67	41272.0
68	41272.0
69	33773.0
70	26274.0
71	21676.5
72	17079.0
73	14061.0
74	11043.0
75	11043.0
76	9016.0
77	6989.0
78	5822.0
79	4655.0
80	3733.0
81	2811.0
82	2811.0
83	2283.5
84	1756.0
85	1374.5
86	993.0
87	710.0
88	427.0
89	427.0
90	261.5
91	96.0
92	61.0
93	26.0
94	15.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2148934.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.84736487748216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.24469260513922	53.78801627751646
2	8.382875286908734	10.704489943313796
3	2.722937655932708	5.215571821709148
4	1.3244209049604307	3.3824313908149497
5	0.7992074091334898	2.551364353186654
6	0.5265183933760563	2.017008718595205
7	0.3576121973919517	1.5982817516065482
8	0.2813171399137553	1.4369086462690586
9	0.21010208691994076	1.2073018144588137
>10	1.0805990285908869	12.04333178610122
>50	0.050014037112251795	2.1565623758915122
>100	0.01882850537040043	2.0840574178948343
>500	2.186873125416374E-4	0.08382219385632446
>1k	4.3737462508327E-4	0.5075873660172486
>5k	2.18687312541635E-4	1.2232641427683246
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9618	0.44757074903184557	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9493	0.44175391147424725	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7172	0.3337468717047615	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4426	0.2059625842394415	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.65347004607866E-5
2	0.0	0.0	0.0	0.0	4.65347004607866E-5
3	0.0	0.0	0.0	0.0	4.65347004607866E-5
4	0.0	0.0	0.0	0.0	4.65347004607866E-5
5	0.0	0.0	0.0	4.65347004607866E-5	4.65347004607866E-5
6	0.0	0.0	0.0	4.65347004607866E-5	4.65347004607866E-5
7	0.0	0.0	0.0	4.65347004607866E-5	9.30694009215732E-5
8	0.0	0.0	0.0	4.65347004607866E-5	9.30694009215732E-5
9	0.0	0.0	0.0	4.65347004607866E-5	1.3960410138235982E-4
10	4.65347004607866E-5	0.0	0.0	4.65347004607866E-5	1.3960410138235982E-4
11	4.65347004607866E-5	0.0	0.0	9.30694009215732E-5	1.3960410138235982E-4
12	4.65347004607866E-5	0.0	0.0	9.30694009215732E-5	1.3960410138235982E-4
13	4.65347004607866E-5	0.0	0.0	1.3960410138235982E-4	1.3960410138235982E-4
14	4.65347004607866E-5	0.0	0.0	1.3960410138235982E-4	1.3960410138235982E-4
15	4.65347004607866E-5	0.0	0.0	2.7920820276471965E-4	2.32673502303933E-4
16	4.65347004607866E-5	0.0	0.0	4.65347004607866E-4	2.32673502303933E-4
17	9.30694009215732E-5	0.0	0.0	6.98020506911799E-4	2.32673502303933E-4
18	9.30694009215732E-5	0.0	0.0	7.445552073725856E-4	2.32673502303933E-4
19	1.3960410138235982E-4	0.0	0.0	9.30694009215732E-4	2.32673502303933E-4
20	1.861388018431464E-4	0.0	0.0	0.0011168328110588786	2.32673502303933E-4
21	1.861388018431464E-4	0.0	0.0	0.0018148533179706775	3.2574290322550623E-4
22	2.32673502303933E-4	0.0	0.0	0.0023267350230393303	3.2574290322550623E-4
23	2.32673502303933E-4	0.0	0.0	0.003071290230411916	4.1881230414707944E-4
24	2.32673502303933E-4	0.0	0.0	0.0039089148387060745	4.1881230414707944E-4
25	2.32673502303933E-4	0.0	0.0	0.005258421152068886	4.65347004607866E-4
26	2.32673502303933E-4	0.0	0.0	0.007724760276490576	4.65347004607866E-4
27	2.32673502303933E-4	0.0	0.0	0.027502007972324882	4.65347004607866E-4
28	2.32673502303933E-4	0.0	0.0	0.08427434253448454	4.65347004607866E-4
29	2.32673502303933E-4	0.0	0.0	0.18283483811043055	4.65347004607866E-4
30	2.32673502303933E-4	0.0	0.0	0.2947042580181616	4.65347004607866E-4
31	2.32673502303933E-4	0.0	0.0	0.6203540918427463	4.65347004607866E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1530	0.0	16.32353	37
CGTAACC	70	0.0025934775	15.857143	3
GGTATCA	4775	0.0	15.381151	1
TATACCG	115	2.2115026E-5	14.478261	5
TACCGTC	90	8.2781457E-4	14.388888	7
TCTTATA	2495	0.0	13.643287	37
AATAGCG	100	0.001833382	12.950001	5
TATACTG	360	0.0	12.847222	5
CGGTCGT	245	1.1459633E-10	12.836736	17
GTCGTGT	160	2.6979033E-6	12.71875	1
CTCTTAT	3805	0.0	12.34954	37
ACTATAC	180	7.4703894E-7	12.333333	3
TGGACCG	210	2.894376E-8	12.333333	5
ATAGCGT	120	5.124664E-4	12.333333	6
GTATTAC	215	4.0168743E-8	12.046512	1
TACTAAG	170	5.3139993E-6	11.970588	2
GTGCTAT	155	2.7222655E-5	11.935483	1
GTCTAGA	280	8.367351E-11	11.892858	1
GATAGTA	140	1.3993902E-4	11.892858	2
TACCGGC	140	1.3993902E-4	11.892858	7
>>END_MODULE