FastQCFastQC Report
Thu 9 Feb 2017
SRR2727129.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727129.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126965
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79150.3721264806896212No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69640.3274148845890741No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52150.2451850406565223No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37390.17579038677176165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC450.003826862420.5555556
CTTATAC16650.017.33333437
GGTATCA30750.016.4243891
ACGACCG1356.572782E-613.70370417
TCGTTAC2850.013.63157823
CGACCGA1254.8481685E-513.3218
TCTTATA25450.013.30255437
GTACTAC2102.044544E-913.2142851
TTACGGA4950.013.08080726
TATTCCG850.00940816713.0588245
TACGCTG1000.001833371612.9499995
CTCTTAT39150.012.85312837
GTTACGG3400.012.51470625
ATTGGAC1705.313923E-611.9705893
TTGCACG1552.7222337E-511.93548426
TACGGAT4850.011.82474227
GATAGTA1100.003746844611.7727272
ACGGATG4900.011.704082528
GTCTAGA2851.1459633E-1011.684211
GTATCAA43250.011.6774562
Produced by FastQC (version 0.11.5)