##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727129.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2126965
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.606508804799326	31.0	31.0	34.0	30.0	34.0
2	31.858872618966462	31.0	31.0	34.0	30.0	34.0
3	31.974677063327324	33.0	31.0	34.0	30.0	34.0
4	35.693864732141805	37.0	35.0	37.0	33.0	37.0
5	35.29241101757669	37.0	35.0	37.0	33.0	37.0
6	35.30247465285042	37.0	35.0	37.0	32.0	37.0
7	35.25341319673807	37.0	35.0	37.0	32.0	37.0
8	35.201702425756885	37.0	35.0	37.0	32.0	37.0
9	37.04267865244609	39.0	37.0	39.0	33.0	39.0
10	36.80616465245079	39.0	37.0	39.0	33.0	39.0
11	36.916450905398065	39.0	37.0	39.0	33.0	39.0
12	36.79804087044216	39.0	37.0	39.0	32.0	39.0
13	36.935686764944414	39.0	37.0	39.0	33.0	39.0
14	37.98833690258185	40.0	37.0	41.0	33.0	41.0
15	37.979776818142284	40.0	37.0	41.0	33.0	41.0
16	37.9144207826645	39.0	37.0	41.0	33.0	41.0
17	37.84683010768865	39.0	37.0	41.0	33.0	41.0
18	37.85688481004624	39.0	37.0	41.0	33.0	41.0
19	37.878576281227005	40.0	37.0	41.0	33.0	41.0
20	37.761469981875585	39.0	37.0	41.0	32.0	41.0
21	37.76779871789146	39.0	37.0	41.0	32.0	41.0
22	37.684819449309224	39.0	37.0	41.0	32.0	41.0
23	37.620768089742896	39.0	37.0	40.0	32.0	41.0
24	37.50618933550858	39.0	36.0	41.0	32.0	41.0
25	37.48726894894838	39.0	36.0	40.0	32.0	41.0
26	37.3546508757784	39.0	36.0	40.0	32.0	41.0
27	37.15821322870851	39.0	36.0	40.0	31.0	41.0
28	37.059250152212186	39.0	36.0	40.0	31.0	41.0
29	36.951124724666364	39.0	36.0	40.0	31.0	41.0
30	36.93287994865924	39.0	36.0	40.0	31.0	41.0
31	36.87856828861782	39.0	36.0	40.0	31.0	41.0
32	36.76520394082648	39.0	35.0	40.0	30.0	41.0
33	36.7466512142889	39.0	35.0	40.0	30.0	41.0
34	36.737169629025395	39.0	35.0	40.0	30.0	41.0
35	36.6362431915899	39.0	35.0	40.0	30.0	41.0
36	36.57968607852033	39.0	35.0	40.0	30.0	41.0
37	36.451599814759525	38.0	35.0	40.0	30.0	41.0
38	36.40327696976678	38.0	35.0	40.0	30.0	41.0
39	36.30902953269095	38.0	35.0	40.0	30.0	41.0
40	36.24441163817929	38.0	35.0	40.0	30.0	41.0
41	36.19090064951703	38.0	35.0	40.0	30.0	41.0
42	36.15351968650166	38.0	35.0	40.0	30.0	41.0
43	34.84061232789444	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	3.0
12	2.0
13	2.0
14	3.0
15	7.0
16	12.0
17	25.0
18	39.0
19	119.0
20	259.0
21	584.0
22	1122.0
23	2225.0
24	3789.0
25	6150.0
26	9907.0
27	14766.0
28	21972.0
29	31331.0
30	43388.0
31	58066.0
32	76934.0
33	100648.0
34	134059.0
35	178838.0
36	244619.0
37	344891.0
38	509887.0
39	343313.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.11564365187016	18.942577804524287	13.087192313930881	25.854586229674677
2	18.34341420756806	20.894702075492543	35.88592195922359	24.875961757715807
3	19.92947697775939	22.662761258412807	30.112625266518258	27.295136497309546
4	15.110074683880553	15.880327132792498	34.0436725569062	34.96592562642075
5	15.554040616559275	35.39663323091823	33.89745482412734	15.151871328395156
6	34.982004875491604	34.179264821000814	15.329119190959888	15.509611112547692
7	28.741046514634704	30.235053233127957	21.60359949505516	19.420300757182183
8	25.86958412573785	35.08172442893983	19.873528713448504	19.175162731873822
9	26.241898667820106	15.123897196239714	20.07042899154429	38.56377514439589
10	17.295160004983625	26.64750007639994	33.18954472687609	22.867795191740345
11	35.198980707251884	21.990159687630026	21.710183289334804	21.100676315783286
12	21.194848058148583	24.939432477732357	30.503886993909163	23.3618324702099
13	30.524385685707102	20.10931068447295	24.54516176805918	24.821141861760772
14	22.02857122707708	20.97871850265519	25.984912774775324	31.007797495492404
15	24.96044833836006	27.719497029805378	22.918148629620138	24.401906002214425
16	24.05751857693944	26.398224700453465	25.178082384994582	24.366174337612513
17	23.693008582651807	26.566022478037954	25.890082817535788	23.850886121774455
18	23.763343543499776	25.605639961165323	26.68830939860317	23.942707096731727
19	24.635384221179006	25.498915120841197	26.964477553697403	22.90122310428239
20	24.10204211164735	25.342165950074403	26.938195974075736	23.617595964202515
21	23.84138902144605	25.58302557870017	27.19325423784594	23.382331162007837
22	24.36349446276737	25.238544122728868	26.483087403882998	23.914874010620768
23	23.80669169450367	25.499996473848892	26.58581593961349	24.107495892033953
24	23.81679999435816	25.51659289174951	26.651684442386212	24.014922671506113
25	24.161892649855545	25.247994207709105	26.46649098598237	24.123622156452974
26	23.746323987465708	25.597224213844612	26.746843507062877	23.909608291626803
27	24.006412893489077	25.882607377178278	26.3536541503974	23.757325578935244
28	23.80692677124447	25.755289814359898	26.433909349707214	24.003874064688418
29	23.709416939159787	26.00724506515152	26.77589899222601	23.50743900346268
30	23.98779481561756	25.803997715054077	26.479373191378325	23.728834277950035
31	23.880693852508152	25.301920812049094	26.813981424235944	24.003403911206814
32	23.476502904373135	25.805737282936015	26.633818610085264	24.08394120260559
33	23.521919730696087	25.308644006836033	26.71595442332149	24.45348183914639
34	23.827895616524014	25.241835197100094	27.01238619347286	23.917882992903035
35	23.605936157858736	25.2285298535707	26.98210830925756	24.18342567931301
36	23.425209159530127	24.88094538462081	27.324709151302446	24.36913630454662
37	23.263946515339935	24.539849033717058	27.78306178051825	24.413142670424758
38	23.133384893498484	24.427012198132083	28.098957904808024	24.340645003561413
39	22.766195024365704	24.4411168025802	28.580724177407713	24.21196399564638
40	22.977623045043057	24.29640356094247	28.542594730049625	24.183378663964852
41	22.410288838791423	24.479528342027255	28.757548901839	24.352633917342317
42	22.12476462941327	24.753627821802425	28.642173237453367	24.479434311330934
43	21.978358835241764	24.473510377462723	28.5901272470398	24.958003540255717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2397.0
1	1897.0
2	1397.0
3	2166.0
4	2935.0
5	2935.0
6	3346.5
7	3758.0
8	3444.5
9	3131.0
10	4347.5
11	5564.0
12	5564.0
13	8760.0
14	11956.0
15	14689.5
16	17423.0
17	16106.0
18	14789.0
19	14789.0
20	17249.5
21	19710.0
22	17628.5
23	15547.0
24	17972.5
25	20398.0
26	20398.0
27	24707.0
28	29016.0
29	33146.5
30	37277.0
31	43469.5
32	49662.0
33	49662.0
34	56861.0
35	64060.0
36	73129.5
37	82199.0
38	92012.5
39	101826.0
40	101826.0
41	111938.0
42	122050.0
43	132903.0
44	143756.0
45	153653.5
46	163551.0
47	163551.0
48	172656.0
49	181761.0
50	184993.5
51	188226.0
52	185001.0
53	181776.0
54	181776.0
55	172529.0
56	163282.0
57	151559.5
58	139837.0
59	126846.0
60	113855.0
61	113855.0
62	99550.0
63	85245.0
64	71932.5
65	58620.0
66	48670.0
67	38720.0
68	38720.0
69	31687.5
70	24655.0
71	20189.0
72	15723.0
73	12603.0
74	9483.0
75	9483.0
76	7650.0
77	5817.0
78	4750.5
79	3684.0
80	2819.0
81	1954.0
82	1954.0
83	1528.0
84	1102.0
85	819.0
86	536.0
87	368.5
88	201.0
89	201.0
90	135.0
91	69.0
92	42.0
93	15.0
94	8.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2126965.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.60670057866407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.59621397363668	57.19270908204251
2	8.427209508391922	11.39471659895047
3	2.6958414293418254	5.467708329632115
4	1.329089463569329	3.594214136231555
5	0.7479668356510025	2.528378495031408
6	0.46677577917433244	1.8934302204007023
7	0.3494085477706437	1.6535651348129021
8	0.24412050867565752	1.3203345708117074
9	0.18852852435423442	1.1471212346898694
>10	0.9225951191358184	10.522221250714631
>50	0.025203051185218973	1.138535057782009
>100	0.0062112741632962	0.6044962480534409
>500	2.0899623750025972E-4	0.10926848945910764
>1k	4.1799247500044816E-4	0.4869041569086731
>5k	2.0899623750022408E-4	0.9463969944789008
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7915	0.3721264806896212	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6964	0.3274148845890741	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5215	0.2451850406565223	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3739	0.17579038677176165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.701534816040697E-5
2	4.701534816040697E-5	0.0	0.0	0.0	4.701534816040697E-5
3	4.701534816040697E-5	0.0	0.0	0.0	4.701534816040697E-5
4	4.701534816040697E-5	0.0	0.0	4.701534816040697E-5	4.701534816040697E-5
5	4.701534816040697E-5	0.0	0.0	4.701534816040697E-5	4.701534816040697E-5
6	4.701534816040697E-5	0.0	0.0	4.701534816040697E-5	1.410460444812209E-4
7	4.701534816040697E-5	0.0	0.0	4.701534816040697E-5	1.410460444812209E-4
8	4.701534816040697E-5	0.0	0.0	4.701534816040697E-5	1.410460444812209E-4
9	9.403069632081394E-5	0.0	0.0	4.701534816040697E-5	1.8806139264162787E-4
10	9.403069632081394E-5	0.0	0.0	4.701534816040697E-5	1.8806139264162787E-4
11	9.403069632081394E-5	0.0	0.0	9.403069632081394E-5	1.8806139264162787E-4
12	9.403069632081394E-5	0.0	0.0	1.8806139264162787E-4	1.8806139264162787E-4
13	9.403069632081394E-5	0.0	0.0	2.350767408020348E-4	1.8806139264162787E-4
14	9.403069632081394E-5	0.0	0.0	2.350767408020348E-4	2.820920889624418E-4
15	9.403069632081394E-5	0.0	0.0	2.350767408020348E-4	3.7612278528325574E-4
16	9.403069632081394E-5	0.0	0.0	2.820920889624418E-4	4.701534816040696E-4
17	9.403069632081394E-5	0.0	0.0	3.7612278528325574E-4	4.701534816040696E-4
18	9.403069632081394E-5	0.0	0.0	4.231381334436627E-4	4.701534816040696E-4
19	9.403069632081394E-5	0.0	0.0	4.701534816040696E-4	4.701534816040696E-4
20	9.403069632081394E-5	0.0	0.0	5.171688297644766E-4	4.701534816040696E-4
21	9.403069632081394E-5	0.0	0.0	9.873223113685463E-4	4.701534816040696E-4
22	9.403069632081394E-5	0.0	0.0	0.001316429748491395	4.701534816040696E-4
23	9.403069632081394E-5	0.0	0.0	0.0020216599708974994	5.171688297644766E-4
24	9.403069632081394E-5	0.0	0.0	0.0031970436749076737	5.171688297644766E-4
25	9.403069632081394E-5	0.0	0.0	0.004936611556842731	5.171688297644766E-4
26	9.403069632081394E-5	0.0	0.0	0.008885900802316916	5.171688297644766E-4
27	9.403069632081394E-5	0.0	0.0	0.032722682319643245	5.171688297644766E-4
28	9.403069632081394E-5	0.0	0.0	0.09732177069204241	5.171688297644766E-4
29	9.403069632081394E-5	0.0	0.0	0.18815542333794869	5.171688297644766E-4
30	9.403069632081394E-5	0.0	0.0	0.2990646296483487	5.171688297644766E-4
31	9.403069632081394E-5	0.0	0.0	0.615054784634444	5.171688297644766E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGC	45	0.0038268624	20.555555	6
CTTATAC	1665	0.0	17.333334	37
GGTATCA	3075	0.0	16.424389	1
ACGACCG	135	6.572782E-6	13.703704	17
TCGTTAC	285	0.0	13.631578	23
CGACCGA	125	4.8481685E-5	13.32	18
TCTTATA	2545	0.0	13.302554	37
GTACTAC	210	2.044544E-9	13.214285	1
TTACGGA	495	0.0	13.080807	26
TATTCCG	85	0.009408167	13.058824	5
TACGCTG	100	0.0018333716	12.949999	5
CTCTTAT	3915	0.0	12.853128	37
GTTACGG	340	0.0	12.514706	25
ATTGGAC	170	5.313923E-6	11.970589	3
TTGCACG	155	2.7222337E-5	11.935484	26
TACGGAT	485	0.0	11.824742	27
GATAGTA	110	0.0037468446	11.772727	2
ACGGATG	490	0.0	11.7040825	28
GTCTAGA	285	1.1459633E-10	11.68421	1
GTATCAA	4325	0.0	11.677456	2
>>END_MODULE