FastQCFastQC Report
Thu 9 Feb 2017
SRR2727128.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727128.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280656
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT274810.8376678322872011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT246670.751892304465936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT199880.6092683902243942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133690.40750996142234974No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45290.1380516579610907No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37260.11357484600640846No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.11098390078081945No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33830.10311961997844334No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33340.10162601626016261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT5000.015.1700017
CTTATAC34400.015.05813937
TACCCGT4650.014.322586
TACTTAC7450.013.65771831
GGTATCA151250.013.503471
ATACCCG4800.013.4895845
TCTTATA52800.013.24431837
TATACAC15250.013.1016437
GTACTAG2401.1295924E-912.3333341
GGACCGT2255.7170837E-912.3333336
TATACGT1050.002645371212.3333334
TCTATAC3650.012.1643843
TAATACT4600.012.0652184
TATACCG1851.0409312E-612.05
GTACTTA9100.011.99450530
CTCGTTA4350.011.90804522
GCACCGT4200.011.8928576
TCGTTAC3750.011.84000123
CCGTTTT5650.011.7876119
CCCGTTT6000.011.7166678