##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727128.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3280656
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.588172609380564	31.0	31.0	34.0	30.0	34.0
2	31.8225275676572	31.0	31.0	34.0	30.0	34.0
3	31.91737384230471	31.0	31.0	34.0	30.0	34.0
4	35.659025511970775	37.0	35.0	37.0	33.0	37.0
5	35.27131250579153	37.0	35.0	37.0	33.0	37.0
6	35.29964159607103	37.0	35.0	37.0	32.0	37.0
7	35.24417067805951	37.0	35.0	37.0	32.0	37.0
8	35.205574738710794	37.0	35.0	37.0	32.0	37.0
9	37.02278964938719	39.0	37.0	39.0	33.0	39.0
10	36.79431522232139	39.0	37.0	39.0	32.0	39.0
11	36.9132777103116	39.0	37.0	39.0	33.0	39.0
12	36.790788488643734	39.0	37.0	39.0	32.0	39.0
13	36.93921977799562	39.0	37.0	39.0	33.0	39.0
14	37.92200401383138	39.0	37.0	41.0	33.0	41.0
15	37.928791375871164	39.0	37.0	41.0	33.0	41.0
16	37.87145741583391	39.0	37.0	41.0	33.0	41.0
17	37.81973849132613	39.0	37.0	41.0	33.0	41.0
18	37.86107961334562	39.0	37.0	41.0	33.0	41.0
19	37.90536923103184	40.0	37.0	41.0	33.0	41.0
20	37.78328267273375	39.0	37.0	41.0	33.0	41.0
21	37.76442760228442	39.0	37.0	41.0	32.0	41.0
22	37.66130249559844	39.0	37.0	41.0	32.0	41.0
23	37.58296694319672	39.0	37.0	40.0	32.0	41.0
24	37.47591548763418	39.0	36.0	40.0	32.0	41.0
25	37.441391294911746	39.0	36.0	40.0	32.0	41.0
26	37.2684975200082	39.0	36.0	40.0	32.0	41.0
27	37.05919517316049	39.0	36.0	40.0	31.0	41.0
28	36.926077589360176	39.0	36.0	40.0	31.0	41.0
29	36.81134687696607	39.0	35.0	40.0	31.0	41.0
30	36.71770310572032	39.0	35.0	40.0	30.0	41.0
31	36.62480491706537	39.0	35.0	40.0	30.0	41.0
32	36.483170134265826	38.0	35.0	40.0	30.0	41.0
33	36.41194017294102	38.0	35.0	40.0	30.0	41.0
34	36.367771872454774	38.0	35.0	40.0	30.0	41.0
35	36.22878716939539	38.0	35.0	40.0	30.0	41.0
36	36.11565674669944	38.0	35.0	40.0	30.0	41.0
37	35.93959622709605	38.0	35.0	40.0	29.0	41.0
38	35.84137288395979	38.0	35.0	40.0	29.0	41.0
39	35.68278265078692	38.0	35.0	40.0	27.0	41.0
40	35.55724647753376	38.0	34.0	40.0	27.0	41.0
41	35.4480085690179	38.0	34.0	40.0	27.0	41.0
42	35.31936509039655	38.0	34.0	40.0	26.0	41.0
43	34.00205934422871	37.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	10.0
11	15.0
12	16.0
13	20.0
14	8.0
15	18.0
16	21.0
17	41.0
18	108.0
19	225.0
20	642.0
21	1130.0
22	2298.0
23	4282.0
24	7252.0
25	11939.0
26	18656.0
27	27898.0
28	40873.0
29	56483.0
30	75279.0
31	97964.0
32	126252.0
33	163474.0
34	212047.0
35	279255.0
36	382257.0
37	532889.0
38	748242.0
39	491060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49614833130935	18.327432074560697	13.272010232099921	24.904409362030034
2	18.422626450319694	21.801188542779247	35.4000846172229	24.37610038967816
3	20.50656941782375	23.17716334781824	30.365634190235124	25.950633044122885
4	15.108167390912062	15.937483235060304	33.52460605439887	35.429743319628756
5	15.424445598685141	35.76918762588945	33.36796055423062	15.438406221194786
6	34.60134192673661	33.91486336878965	15.048606132432049	16.43518857204169
7	28.146139064869953	30.80326007969138	21.76817685243439	19.282424003004277
8	26.560511068518007	34.500447471481316	19.799210889529412	19.139830570471272
9	26.513935017874473	14.894246760404018	20.183951014675113	38.4078672070464
10	17.854782701996186	26.886360532771498	32.14128515760263	23.117571607629696
11	34.39199355250901	22.43554337912905	21.261509893143323	21.910953175218616
12	21.310829297555124	26.001842314463936	30.229502879911823	22.457825508069117
13	31.043547388083358	19.929184894728373	24.834636731190347	24.192630985997923
14	21.82158080579006	21.499206256309712	27.068885003487107	29.610327934413117
15	24.96348291317346	27.742378353597573	23.869890656015137	23.424248077213825
16	23.003387127452555	26.37862671368165	26.20350929814037	24.414476860725415
17	23.21267453826308	26.625650479660166	26.62180978438459	23.53986519769217
18	23.243857326095757	25.21148209382514	27.687511278232158	23.857149301846945
19	24.48769392462971	25.07422296028599	28.46753819967714	21.97054491540716
20	23.84712081973849	25.37641861871528	27.999430601684537	22.777029959861686
21	23.073159758292245	25.998946552152987	28.571297935534844	22.356595754019928
22	23.5624521437176	25.728726205978315	27.71253676094049	22.99628488936359
23	22.83549387683439	25.928716695685257	28.08651684297287	23.14927258450749
24	22.892525153505886	25.859462253890687	28.137848040148068	23.110164552455362
25	23.191459269121786	25.546780887724896	27.935967684511876	23.32579215864144
26	22.698996786008653	26.134254856345805	28.355975146434126	22.810773211211416
27	23.29204890729171	26.203326407889154	27.999643973644297	22.504980711174838
28	22.909930209080134	26.477753229841838	27.78807043469355	22.82424612638448
29	22.86131188396467	26.573770611731312	28.125533429899384	22.439384074404632
30	22.983787388863693	26.45074643607864	27.984067820582226	22.581398354475446
31	22.72124843324018	25.98559556381407	28.348872908345164	22.944283094600593
32	22.289535995239977	26.544020464199843	28.068867933730328	23.097575606829853
33	22.408688993908534	26.013303436873603	28.095539428699627	23.482468140518236
34	22.68567627937827	26.09215961685712	28.349634951058565	22.872529152706043
35	22.409268146370724	26.163364887998007	28.251483849571553	23.175883116059715
36	22.219732882691755	25.819531215708082	28.596536790202936	23.364199111397234
37	21.941191030086664	25.445551133675703	28.9447598285221	23.66849800771553
38	21.93381445662087	25.330939909579058	29.21443150394311	23.520814129856955
39	21.432939021951707	25.36559761218488	29.876311323101234	23.32515204276218
40	21.83151784277291	25.266532059441772	29.69970030384167	23.20224979394365
41	21.228498202798466	25.422567925439303	29.866008505615948	23.482925366146283
42	20.81662935705542	25.755214810696398	29.672541101535792	23.755614730712395
43	20.634623075384923	25.466766402816997	29.46752112992036	24.431089391877723
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3003.0
1	2613.0
2	2223.0
3	4543.0
4	6863.0
5	6863.0
6	8627.0
7	10391.0
8	10148.0
9	9905.0
10	14332.5
11	18760.0
12	18760.0
13	29912.5
14	41065.0
15	52992.0
16	64919.0
17	57455.0
18	49991.0
19	49991.0
20	51882.5
21	53774.0
22	41494.5
23	29215.0
24	32017.5
25	34820.0
26	34820.0
27	39815.0
28	44810.0
29	50004.0
30	55198.0
31	62356.0
32	69514.0
33	69514.0
34	78432.0
35	87350.0
36	99305.5
37	111261.0
38	124921.5
39	138582.0
40	138582.0
41	155162.0
42	171742.0
43	189753.0
44	207764.0
45	226316.0
46	244868.0
47	244868.0
48	260904.0
49	276940.0
50	280779.0
51	284618.0
52	278728.5
53	272839.0
54	272839.0
55	257370.5
56	241902.0
57	224540.0
58	207178.0
59	189127.0
60	171076.0
61	171076.0
62	149628.5
63	128181.0
64	108220.0
65	88259.0
66	73056.5
67	57854.0
68	57854.0
69	47128.5
70	36403.0
71	29785.0
72	23167.0
73	19029.5
74	14892.0
75	14892.0
76	12018.0
77	9144.0
78	7394.5
79	5645.0
80	4434.0
81	3223.0
82	3223.0
83	2542.0
84	1861.0
85	1383.5
86	906.0
87	660.0
88	414.0
89	414.0
90	256.0
91	98.0
92	64.0
93	30.0
94	18.5
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3280656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.40274533261842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.67115794362631	48.28222583734283
2	8.955795627901592	10.460861026146286
3	3.040894970426629	5.327898436231999
4	1.4492842005699824	3.385687043219047
5	0.8856358131758219	2.586178142717698
6	0.5811316138469352	2.0363808988941665
7	0.425313629323734	1.7387638505919998
8	0.3079624953775755	1.4388684151627378
9	0.2416872920713254	1.2703681232074606
>10	1.3466437684835755	14.048624381578968
>50	0.06680962781126362	2.6203157589139545
>100	0.025128117033097114	2.655061795651042
>500	0.0016163655301937253	0.5930463196763033
>1k	7.299715280809726E-4	0.9519497285423429
>5k	0.0	0.0
>10k+	2.0856329373742071E-4	2.60377024212319
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27481	0.8376678322872011	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24667	0.751892304465936	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19988	0.6092683902243942	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13369	0.40750996142234974	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4529	0.1380516579610907	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3726	0.11357484600640846	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3641	0.11098390078081945	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3383	0.10311961997844334	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3334	0.10162601626016261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.048170853634151E-5
2	0.0	0.0	0.0	0.0	3.048170853634151E-5
3	0.0	0.0	0.0	0.0	3.048170853634151E-5
4	0.0	0.0	0.0	0.0	3.048170853634151E-5
5	9.144512560902454E-5	0.0	0.0	0.0	3.048170853634151E-5
6	9.144512560902454E-5	0.0	0.0	0.0	6.096341707268302E-5
7	9.144512560902454E-5	0.0	0.0	0.0	6.096341707268302E-5
8	9.144512560902454E-5	0.0	0.0	0.0	9.144512560902454E-5
9	1.5240854268170755E-4	0.0	0.0	3.048170853634151E-5	9.144512560902454E-5
10	2.1337195975439059E-4	0.0	0.0	3.048170853634151E-5	9.144512560902454E-5
11	3.048170853634151E-4	0.0	0.0	3.048170853634151E-5	9.144512560902454E-5
12	3.048170853634151E-4	0.0	0.0	6.096341707268302E-5	1.2192683414536605E-4
13	3.048170853634151E-4	0.0	0.0	9.144512560902454E-5	1.2192683414536605E-4
14	3.6578050243609816E-4	0.0	0.0	1.5240854268170755E-4	2.438536682907321E-4
15	3.6578050243609816E-4	0.0	0.0	3.048170853634151E-4	2.438536682907321E-4
16	4.2674391950878117E-4	0.0	0.0	7.620427134085379E-4	2.438536682907321E-4
17	4.572256280451227E-4	0.0	0.0	0.0011887866329173189	2.438536682907321E-4
18	4.572256280451227E-4	0.0	0.0	0.001371676884135368	3.048170853634151E-4
19	4.877073365814642E-4	0.0	0.0	0.002133719597543906	3.048170853634151E-4
20	4.877073365814642E-4	0.0	0.0	0.0030176891450978097	3.048170853634151E-4
21	5.486707536541472E-4	0.0	0.0	0.005578152662150497	3.3529879389975663E-4
22	5.486707536541472E-4	0.0	0.0	0.00887017718407538	3.3529879389975663E-4
23	5.486707536541472E-4	0.0	0.0	0.010607634570646847	4.572256280451227E-4
24	5.791524621904888E-4	0.0	0.0	0.014021585926717095	4.877073365814642E-4
25	6.096341707268302E-4	0.0	0.0	0.01758794582546905	4.877073365814642E-4
26	6.096341707268302E-4	0.0	0.0	0.023775732658346378	4.877073365814642E-4
27	6.401158792631717E-4	0.0	0.0	0.05285528260201618	4.877073365814642E-4
28	6.401158792631717E-4	0.0	0.0	0.13555215786111072	4.877073365814642E-4
29	6.401158792631717E-4	0.0	0.0	0.30624972566462316	4.877073365814642E-4
30	6.705975877995133E-4	0.0	0.0	0.4995342394935647	4.877073365814642E-4
31	6.705975877995133E-4	0.0	0.0	0.9300578908608522	4.877073365814642E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGTT	500	0.0	15.170001	7
CTTATAC	3440	0.0	15.058139	37
TACCCGT	465	0.0	14.32258	6
TACTTAC	745	0.0	13.657718	31
GGTATCA	15125	0.0	13.50347	1
ATACCCG	480	0.0	13.489584	5
TCTTATA	5280	0.0	13.244318	37
TATACAC	1525	0.0	13.10164	37
GTACTAG	240	1.1295924E-9	12.333334	1
GGACCGT	225	5.7170837E-9	12.333333	6
TATACGT	105	0.0026453712	12.333333	4
TCTATAC	365	0.0	12.164384	3
TAATACT	460	0.0	12.065218	4
TATACCG	185	1.0409312E-6	12.0	5
GTACTTA	910	0.0	11.994505	30
CTCGTTA	435	0.0	11.908045	22
GCACCGT	420	0.0	11.892857	6
TCGTTAC	375	0.0	11.840001	23
CCGTTTT	565	0.0	11.787611	9
CCCGTTT	600	0.0	11.716667	8
>>END_MODULE