FastQCFastQC Report
Thu 9 Feb 2017
SRR2727127.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727127.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2702364
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT159090.5887067767332602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158660.5871155773241502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117490.43476748506122787No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80330.2972582524041913No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.10357598014183136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC22800.016.79605337
GGTATCA86350.014.632891
TATACAC9650.013.99481937
TCTTATA35600.013.51123637
ACGGATG8150.013.39263728
TAATCGA1409.569443E-613.21428520
GTTACGG6600.013.17424225
CGTTACG3250.013.09230724
TACGGAT8200.013.08536627
GTACTAG1703.7364953E-713.0588231
TCGTTAC3750.012.82666623
GCACCGT2753.6379788E-1212.7818186
AGTGTAC8700.012.7586227
TAGTACT3100.012.5322584
CGAACGG1050.00264516312.33333329
TTACGGA8950.012.19553126
CTCTTAT53200.011.47556437
CGACCGA2103.8465805E-711.4523818
TAAGGCG2103.8465805E-711.452385
CTCGTTA4400.011.35227322
Produced by FastQC (version 0.11.5)