##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2727127.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2702364
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59406911874196	31.0	31.0	34.0	30.0	34.0
2	31.838008499225122	31.0	31.0	34.0	30.0	34.0
3	31.939689101838244	31.0	31.0	34.0	30.0	34.0
4	35.67506708940765	37.0	35.0	37.0	33.0	37.0
5	35.282624768535996	37.0	35.0	37.0	33.0	37.0
6	35.30218949038694	37.0	35.0	37.0	32.0	37.0
7	35.24983199894611	37.0	35.0	37.0	32.0	37.0
8	35.21149667476328	37.0	35.0	37.0	32.0	37.0
9	37.041081438325854	39.0	37.0	39.0	33.0	39.0
10	36.805954712244535	39.0	37.0	39.0	33.0	39.0
11	36.92008515507163	39.0	37.0	39.0	33.0	39.0
12	36.80900130404342	39.0	37.0	39.0	32.0	39.0
13	36.93901080683431	39.0	37.0	39.0	33.0	39.0
14	37.948801863849575	39.0	37.0	41.0	33.0	41.0
15	37.95177370628087	39.0	37.0	41.0	33.0	41.0
16	37.890749358709634	39.0	37.0	41.0	33.0	41.0
17	37.82347196750697	39.0	37.0	41.0	33.0	41.0
18	37.85476271886393	39.0	37.0	41.0	33.0	41.0
19	37.902862086676706	40.0	37.0	41.0	33.0	41.0
20	37.77956855553138	39.0	37.0	41.0	33.0	41.0
21	37.77068818264305	39.0	37.0	41.0	33.0	41.0
22	37.670313843730895	39.0	37.0	41.0	32.0	41.0
23	37.60697781645996	39.0	37.0	40.0	32.0	41.0
24	37.49769794150603	39.0	36.0	40.0	32.0	41.0
25	37.47905648535875	39.0	36.0	40.0	32.0	41.0
26	37.319572418815525	39.0	36.0	40.0	32.0	41.0
27	37.1226252273935	39.0	36.0	40.0	31.0	41.0
28	37.001090526664804	39.0	36.0	40.0	31.0	41.0
29	36.90643562451246	39.0	36.0	40.0	31.0	41.0
30	36.832659848932266	39.0	36.0	40.0	31.0	41.0
31	36.76823995583126	39.0	35.0	40.0	30.0	41.0
32	36.646262309592636	39.0	35.0	40.0	30.0	41.0
33	36.5942049257613	39.0	35.0	40.0	30.0	41.0
34	36.56624311158674	38.0	35.0	40.0	30.0	41.0
35	36.45355880999007	38.0	35.0	40.0	30.0	41.0
36	36.35120102251214	38.0	35.0	40.0	30.0	41.0
37	36.199902011720106	38.0	35.0	40.0	30.0	41.0
38	36.124749663627846	38.0	35.0	40.0	30.0	41.0
39	35.989900694355015	38.0	35.0	40.0	29.0	41.0
40	35.900304326138155	38.0	35.0	40.0	29.0	41.0
41	35.81892742798527	38.0	35.0	40.0	28.0	41.0
42	35.7243850199307	38.0	35.0	40.0	28.0	41.0
43	34.40434782286916	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	9.0
11	10.0
12	7.0
13	8.0
14	12.0
15	4.0
16	8.0
17	19.0
18	75.0
19	175.0
20	393.0
21	872.0
22	1732.0
23	3033.0
24	5240.0
25	8523.0
26	13602.0
27	20533.0
28	30362.0
29	42763.0
30	58118.0
31	77776.0
32	100434.0
33	131168.0
34	172748.0
35	229963.0
36	314303.0
37	440431.0
38	635629.0
39	414413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.790201468047975	18.670726815484517	13.159515150438652	25.379556566028853
2	18.421944638102048	21.33591181646884	35.70492354101816	24.537220004410955
3	20.134963313602462	23.001194509695956	30.391316639801303	26.47252553690028
4	15.123980337215858	15.916619670777141	33.69786601656919	35.2615339754378
5	15.445957687417385	35.71979940526147	33.60775972444866	15.226483182872478
6	34.81869947941876	33.9839488684722	15.20298523811004	15.994366413999003
7	28.443503539863617	30.7149962033242	21.63690753725257	19.204592719559617
8	26.31022319717107	34.82780262022437	19.811098726892453	19.05087545571211
9	26.563371921769235	15.000421852866602	20.09858775501746	38.337618470346705
10	17.59533504738814	26.784030574711622	32.62162314181214	22.99901123608811
11	34.79342531206011	22.286375928631376	21.420541422251034	21.499657337057478
12	21.29901819295994	25.426663469465993	30.36293408289927	22.911384254674797
13	30.87134079642861	19.94194712481368	24.70603516032629	24.480676918431417
14	21.95966198483994	21.283772282342422	26.53839379150995	30.218171941307688
15	25.14139471958626	27.565531512409137	23.435295911283603	23.857777856721004
16	23.518889387218007	26.373242094699307	25.798560075548664	24.309308442534018
17	23.501349189080376	26.558302286442537	26.197840113323	23.742508411154088
18	23.474187785213243	25.367456049592136	27.244146236406348	23.914209928788278
19	24.58388285219904	25.237532767606435	27.775717852961336	22.402866527233193
20	23.8978168744107	25.164559622611904	27.597022458854543	23.34060104412285
21	23.340082979198954	25.81584124122435	28.08296735746924	22.76110842210746
22	23.94740308855506	25.363237520926123	27.10730308722289	23.58205630329593
23	23.29671354414135	25.643695667941106	27.44374925065609	23.61584153726145
24	23.21060375286231	25.567577128765777	27.60719873414536	23.61462038422655
25	23.645445247198378	25.298738437901036	27.240371763389387	23.815444551511195
26	23.14122005769763	25.723995731145028	27.650938215577174	23.483845995580165
27	23.672865683527462	26.002418623101846	27.257652929065067	23.067062764305625
28	23.31440176082867	26.143443296313894	27.0107949928285	23.53135995002894
29	23.163607863337436	26.339974925657682	27.52231009590122	22.974107115103664
30	23.594156819732646	26.011003698983558	27.22712410319261	23.167715378091184
31	23.32143264193869	25.52169137836354	27.664444908235904	23.492431071461876
32	22.8267546488926	26.134969234344446	27.370110022187983	23.66816609457497
33	22.934919204074657	25.56717007775414	27.485934537316215	24.011976180854987
34	23.311700422296923	25.663234116499478	27.79499726905776	23.23006819214584
35	23.076350928298332	25.658682546096678	27.624775936920415	23.640190588684572
36	22.79141522015539	25.342884970344482	28.060357524004907	23.805342285495218
37	22.548738807947412	25.03970597595291	28.359762045379526	24.051793170720153
38	22.655682210094568	24.794032188113814	28.610061412896265	23.940224188895353
39	22.09280467028128	24.903639924155296	29.236364901249424	23.767190504314
40	22.536453268323587	24.716285444891952	29.155842810220978	23.591418476563483
41	21.890426308224946	24.90822849919552	29.32606414235832	23.875281050221215
42	21.561270058363714	25.196013564419893	29.170126600265544	24.07258977695085
43	21.29069214954018	24.87799571042243	28.95017103543416	24.88114110460323
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2736.0
1	2328.0
2	1920.0
3	3344.0
4	4768.0
5	4768.0
6	5765.0
7	6762.0
8	6336.0
9	5910.0
10	8507.5
11	11105.0
12	11105.0
13	17820.0
14	24535.0
15	30855.0
16	37175.0
17	33548.0
18	29921.0
19	29921.0
20	32579.0
21	35237.0
22	28610.5
23	21984.0
24	25161.5
25	28339.0
26	28339.0
27	32895.5
28	37452.0
29	42268.5
30	47085.0
31	53342.0
32	59599.0
33	59599.0
34	67785.0
35	75971.0
36	86395.5
37	96820.0
38	108078.5
39	119337.0
40	119337.0
41	133135.5
42	146934.0
43	160853.0
44	174772.0
45	189669.5
46	204567.0
47	204567.0
48	217724.5
49	230882.0
50	235928.5
51	240975.0
52	236547.5
53	232120.0
54	232120.0
55	217888.5
56	203657.0
57	188853.5
58	174050.0
59	158222.5
60	142395.0
61	142395.0
62	124364.0
63	106333.0
64	89863.0
65	73393.0
66	60572.0
67	47751.0
68	47751.0
69	38757.5
70	29764.0
71	24288.0
72	18812.0
73	15280.0
74	11748.0
75	11748.0
76	9544.5
77	7341.0
78	5909.5
79	4478.0
80	3583.0
81	2688.0
82	2688.0
83	2195.0
84	1702.0
85	1278.5
86	855.0
87	615.0
88	375.0
89	375.0
90	228.0
91	81.0
92	52.0
93	23.0
94	15.0
95	7.0
96	7.0
97	5.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2702364.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.64483206287939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.28372994449069	51.340115459983274
2	8.805421092410235	10.856174089691299
3	2.930078668415769	5.41872622336546
4	1.4552934518339822	3.5884528176205555
5	0.8229977392772508	2.536677871293776
6	0.5576810404127894	2.062689244853895
7	0.3813569827060265	1.6456081008443584
8	0.2970985118573312	1.4651670295661252
9	0.2154481946542292	1.1953140999939458
>10	1.1744361678656787	12.723646340741876
>50	0.05523098027472364	2.300021617402978
>100	0.01978855713440505	2.1249991681557607
>500	8.392233890581694E-4	0.354265568326355
>1k	3.596671667255351E-4	0.48297183455607867
>5k	5.994452778758919E-5	0.29684106709938635
>10k+	1.7983358336276755E-4	1.6083294665048788
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15909	0.5887067767332602	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15866	0.5871155773241502	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11749	0.43476748506122787	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8033	0.2972582524041913	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2799	0.10357598014183136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.700463742116162E-5
2	0.0	0.0	0.0	0.0	3.700463742116162E-5
3	0.0	0.0	0.0	0.0	3.700463742116162E-5
4	0.0	0.0	0.0	0.0	7.400927484232325E-5
5	3.700463742116162E-5	0.0	0.0	0.0	7.400927484232325E-5
6	7.400927484232325E-5	0.0	0.0	0.0	7.400927484232325E-5
7	1.1101391226348486E-4	0.0	0.0	0.0	7.400927484232325E-5
8	1.480185496846465E-4	0.0	0.0	0.0	7.400927484232325E-5
9	1.850231871058081E-4	0.0	0.0	0.0	7.400927484232325E-5
10	2.2202782452696972E-4	0.0	0.0	0.0	7.400927484232325E-5
11	2.2202782452696972E-4	0.0	0.0	0.0	7.400927484232325E-5
12	2.2202782452696972E-4	0.0	0.0	0.0	7.400927484232325E-5
13	2.5903246194813135E-4	0.0	0.0	0.0	7.400927484232325E-5
14	2.5903246194813135E-4	0.0	0.0	1.850231871058081E-4	7.400927484232325E-5
15	2.5903246194813135E-4	0.0	0.0	2.2202782452696972E-4	7.400927484232325E-5
16	2.96037099369293E-4	0.0	0.0	4.070510116327778E-4	2.2202782452696972E-4
17	2.96037099369293E-4	0.0	0.0	5.180649238962627E-4	2.2202782452696972E-4
18	2.96037099369293E-4	0.0	0.0	5.92074198738586E-4	2.2202782452696972E-4
19	3.3304173679045456E-4	0.0	0.0	0.0010361298477925254	2.2202782452696972E-4
20	3.3304173679045456E-4	0.0	0.0	0.001554194771688788	2.2202782452696972E-4
21	3.3304173679045456E-4	0.0	0.0	0.0024793107072178283	2.2202782452696972E-4
22	4.070510116327778E-4	0.0	0.0	0.0037744730169584854	2.5903246194813135E-4
23	4.070510116327778E-4	0.0	0.0	0.0052176538763837885	4.070510116327778E-4
24	4.070510116327778E-4	0.0	0.0	0.007067885747441869	4.070510116327778E-4
25	4.070510116327778E-4	0.0	0.0	0.008770099068815304	4.070510116327778E-4
26	4.070510116327778E-4	0.0	0.0	0.012396553536089143	4.070510116327778E-4
27	4.070510116327778E-4	0.0	0.0	0.035117400912682374	4.070510116327778E-4
28	4.070510116327778E-4	0.0	0.0	0.10279888275598698	4.070510116327778E-4
29	4.070510116327778E-4	0.0	0.0	0.2147009063175797	4.070510116327778E-4
30	4.070510116327778E-4	0.0	0.0	0.34754755465954995	4.070510116327778E-4
31	4.070510116327778E-4	0.0	0.0	0.7155956784504234	4.4405564905393945E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2280	0.0	16.796053	37
GGTATCA	8635	0.0	14.63289	1
TATACAC	965	0.0	13.994819	37
TCTTATA	3560	0.0	13.511236	37
ACGGATG	815	0.0	13.392637	28
TAATCGA	140	9.569443E-6	13.214285	20
GTTACGG	660	0.0	13.174242	25
CGTTACG	325	0.0	13.092307	24
TACGGAT	820	0.0	13.085366	27
GTACTAG	170	3.7364953E-7	13.058823	1
TCGTTAC	375	0.0	12.826666	23
GCACCGT	275	3.6379788E-12	12.781818	6
AGTGTAC	870	0.0	12.75862	27
TAGTACT	310	0.0	12.532258	4
CGAACGG	105	0.002645163	12.333333	29
TTACGGA	895	0.0	12.195531	26
CTCTTAT	5320	0.0	11.475564	37
CGACCGA	210	3.8465805E-7	11.45238	18
TAAGGCG	210	3.8465805E-7	11.45238	5
CTCGTTA	440	0.0	11.352273	22
>>END_MODULE