FastQCFastQC Report
Thu 9 Feb 2017
SRR2727126.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2727126.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5520107
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT186130.33718549296236466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT173360.31405188341457874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT131720.23861856300973877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95750.17345678263120623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94300.016.8520681
CTTATAC50700.015.90927137
TCTTATA76550.013.07446237
TATACGG1703.7388781E-713.0588242
TCGTTAC6500.012.80769323
GTATCAA126000.012.6416662
GTATACG2859.094947E-1212.3333331
CTCTTAT115550.012.11986237
TATACAC20700.012.06521837
ACCGTGC4650.011.9354848
TATACTG7700.011.7727275
ATACCCG2901.5643309E-1011.48275855
TACTTAC12500.011.131
AACGCAG138850.011.0986677
CGTTACG6200.011.04032324
CTCGTTA8750.010.99428522
GTACTAG4050.010.9629631
TATTCCG3053.765308E-1010.9180335
TAGACAG10000.010.9155
GTAGTAC4750.010.9052633
Produced by FastQC (version 0.11.5)